Reaction of human periapical tissue to pulp extirpation and immediate root canal filling with calcium hydroxide

Holland, R; Mello, W. de; Nery, Mauro Juvenal; Bernabé, Pedro Felício Estrada; Souza, Valdir de

doi:10.1016/s0099-2399(77)80017-4

Cited by 58 publications

(33 citation statements)

References 1 publication

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“…Sealapex was used in this study as a reference of calcium hydroxide-containing root canal sealer because of the presence of numerous studies in the literature (11)(12)(13)(14)(15)(16) and the clinical acceptance based on the favorable healing process observed with its use (9,10). This material was not cytotoxic compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

“…The ions releasing must be interpreted depending on the experimental model used. On the one hand, products with calcium hydroxide are more cytotoxic than other materials in cell culture (28,29); on the other hand, they stimulate repair through the formation of mineralized tissue when evaluated in vivo (10,14).…”

Section: Discussionmentioning

confidence: 99%

“…The diffusion of calcium and hydroxyl ions from the sealer raises the pH at the surface of the root adjacent to periodontal tissues. It also favors the repair (11), the antimicrobial action (12), the degradation of bacterial lipopolysaccharides (13), the induction of hard tissue formation (14), and also the control of inflammatory root resorption (15,16).…”

mentioning

confidence: 99%

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Evaluation of the Effects of Endodontic Materials on Fibroblast Viability and Cytokine Production

Gomes‐Filho

Watanabe

Gomes

et al. 2009

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Effects of Endodontic Materials on Fibroblast Viability and Cytokine Production

Gomes‐Filho

Watanabe

Gomes

et al. 2009

Journal of Endodontics

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“…Endodontic treatment aimed to restore the normality of apical and periapical tissues [1][2][3][4] through a root canal system deep cleaning and disinfection, to control pathogenic microorganisms and the complete three-dimensional sealing using root canal sealers [5][6][7]. To achieve this goal, irrigation and aspiration of the root canal system, coupled with instrumentation, composes an important stage of radical endodontic treatment, the chemical mechanical preparation.…”

Section: Introductionmentioning

confidence: 99%

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

Martins¹

2017

J. Dent. Sci. Ther

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The aim of this study was to evaluate the ethanolic extract of Psidium cattleianum biocompatibility by structural and functional cell viability maintenance. Cell viability was analyzed after 1 h, 3 h, 6 h, 10 h and 24 h of incubation by Exclusion Method with trypan blue and MTT assay, using human mononuclear cells (PBMC) and human periodontal ligament (PDL) cells in culture. Positive control used was RPMI cell culture medium and negative control was ethanol 80%. The obtained data were subjected to statistical analysis at a significance level of 5%. By trypan blue assay, ethanolic extract presented a homogeneous performance of PBMC from the 1 h, with average viability between 30 and 50% (p>0.05). By MTT assay, the results showed the efficacy of the ethanolic extract of Psidium cattleianum in maintaining the functional viability of the periodontal ligament cells to 10 h (p>0.05), presenting values greater than the RPMI control. In both methods, it can be observed that the extract properties can neutralize the cytotoxic effect of its diluent ethanol 80%, which showed average viability nearly zero (p <0.05). Thus, biocompatibility by structural and functional cell viability maintenance was considered acceptable to ethanolic extract of Psidium cattleianum and further studies should be performed to indicate its use as an alternative to irrigation solution traditionally used in everyday practice.

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“…Endodontic treatment expected to reestablish the typicality of apical and periapical tissues [1][2][3][4] through a root channel framework profound cleaning and cleansing, keeping in mind the end goal to control pathogenic microorganisms and the entire three-dimensional fixing utilizing root trench sealers [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

MTT Assay of Psidium cattleianum

Martinz¹,

John²,

Hidalgo³

2017

Periodon Prosthodon

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The point of this review was to assess the ethanolic concentrate of Psidium cattleianum biocompatibility by basic and utilitarian cell reasonability support. Cell practicality was broke down after 1, 3, 6, 10 and 24 hours of hatching by Exclusion Method with trypan blue and MTT measure, utilizing human mononuclear cells [PBMC] and human periodontal tendon [PDL] cells in culture. Positive control utilized was RPMI cell culture medium and negative control was ethanol 80%. The got information were subjected to measurable examination at a criticalness level of 5%. By trypan blue examine, ethanolic remove displayed a homogeneous execution of PBMC from the 1 h, with normal reasonability in the vicinity of 30 and half (p>0.05). By MTT examine, the outcomes demonstrated the adequacy of the ethanolic concentrate of Psidium cattleianum in keeping up the utilitarian reasonability of the periodontal tendon cells to 10 h (p>0.05), displaying values more noteworthy than the RPMI control. In both techniques, it can be watched that the concentrate properties can kill the cytotoxic impact of its diluent ethanol 80%, which indicated normal practicality almost zero (p<0.05). The biocompatibility by auxiliary and useful cell feasibility support was viewed as satisfactory to ethanolic concentrate of Psidium cattleianum and further reviews ought to be performed to demonstrate its utilization as another option to water system arrangement generally utilized as a part of regular practice.

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Reaction of human periapical tissue to pulp extirpation and immediate root canal filling with calcium hydroxide

Cited by 58 publications

References 1 publication

Evaluation of the Effects of Endodontic Materials on Fibroblast Viability and Cytokine Production

Evaluation of the Effects of Endodontic Materials on Fibroblast Viability and Cytokine Production

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

MTT Assay of Psidium cattleianum

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