Reaktion von Epidermis und subepidermalem Bindegewebe auf Hornschichtabrisse

Schellander, F

doi:10.1007/bf00504457

Cited by 15 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After six to eight strippings of guinea-pig ears, peaks in the 3H-thymidine labelling index were seen at 30 and 60 hr, with a return to normal by 3-4 days (Christophers & Braun-Falco, 1967). After twenty-four strippings, the labelling index in guinea-pig ears was highest at 24 hr, but did not return to normal within 9 days (Schellander, 1969). These variations in results may be attributed to differences in the epidermal thickness of humans, guinea-pigs and mice, differences in the number of tape applications (Pinkus, 1952;Schellander, 1969), differences in the normal growth kinetics of the assay tissues, or to differences in the methods of estimating cell proliferation.…”

Section: Nature Of the Reactionmentioning

confidence: 93%

“…After twenty-four strippings, the labelling index in guinea-pig ears was highest at 24 hr, but did not return to normal within 9 days (Schellander, 1969). These variations in results may be attributed to differences in the epidermal thickness of humans, guinea-pigs and mice, differences in the number of tape applications (Pinkus, 1952;Schellander, 1969), differences in the normal growth kinetics of the assay tissues, or to differences in the methods of estimating cell proliferation. In our experiments, when stripped mice were not injected with Colcemid, the number of mitoses observed was about normal during the initial rise in the mitotic rate, indicating that the mitotic duration was very short during this period.…”

Section: Nature Of the Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Epidermal Regeneration After Cellophane Tape Stripping of Hairless Mouse Skin

Hennings

Elgjo

1970

Cell Proliferation

View full text Add to dashboard Cite

After repeated applications of cellophane tape to the dorsal skin of hairless mice, the proliferative response in the treated epidermis was estimated by three different methods. The mitotic rate was determined in the interfollicular epidermis using the Colcemid technique, and the DNA synthetic activity was estimated after 3H‐thymidine injection by counting labelled interfollicular cells in autoradiographs and by determining the specific activity of epidermal DNA. An initial 40–50% inhibition of DNA synthesis and mitosis was followed by an increase in the labelling index and the mitotic rate 8–10 hr after tape stripping. By 24 hr, peak values 5–6 times the controls were attained for both parameters. The labelling index and the mitotic rate were nearly normal at 3–4 days, but a second small peak was seen on day 5. Normal values were found on days 6 and 8. A similar pattern of response was found biochemically, but the peak of DNA specific activity was much broader and the extent of the increase was only about half as great as the increase in the labelling index. Possible reasons for these differences are discussed.

show abstract

Section: Nature Of the Reactionmentioning

confidence: 93%

Section: Nature Of the Reactionmentioning

confidence: 99%

Epidermal Regeneration After Cellophane Tape Stripping of Hairless Mouse Skin

Hennings

Elgjo

1970

Cell Proliferation

View full text Add to dashboard Cite

show abstract

“…The aim of this report was to use MFF, the stathmokinetic method and methods based on 3H-TdR incorporation to study in detail the cell kinetics of epidermis stimulated to proliferate by removing the surface layers of epidermis with adhesive tape (Pinkus, 1951(Pinkus, , 1952Christophers & Braun-Falco, 1967;Schellander, 1969;Hennings & Elgjo, 1970).…”

Section: Introductionmentioning

confidence: 99%

Regenerative Proliferation of Mouse Epidermal Cells Following Adhesive Tape Stripping

Clausen¹,

Lindmo²

1976

Cell Proliferation

View full text Add to dashboard Cite

The proliferating cells of mouse epidermis (basal cells) can be separated from the non‐proliferating cells (differentiating cells) (Laerum, 1969) and brought into a mono‐disperse suspension. This makes it possible to determine the cell cycle distributions (e.g. the relative number of cells in the G^ S and (G2+ M) phases of the cell cycle) of the basal cell population by means of micro‐flow fluorometry. To study the regenerative cell proliferation in epidermis in more detail, changes in cell cycle distributions were observed by means of micro‐flow fluorometry during the first 48 hr following adhesive tape stripping. 3H‐TdR uptake (LI and grain count distribution) and mitotic rate (colcemid method) were also observed. An initial accumulation of G2 cells was observed 2 hr after stripping, followed by a subsequent decrease to less than half the control level. This was followed by an increase of cells entering mitosis from an initial depression to a first peak between 5 and 9 hr which could be satisfactorily explained by the changes in the G2 pool. After an initial depression of the S phase parameters, three peaks with intervals of about 12 hr followed. The cells in these peaks could be followed as cohorts through the G2 phase and mitosis, indicating a partial synchrony of cell cycle passage, with a shortening of the mean generation time of basal cells from 83‐3 hr to about 12 hr. The oscillations of the proportion of cells in G2 phase indicated a rapid passage through this cell cycle phase. The S phase duration was within the normal range but showed a moderate decrease and the Gj phase duration was decreased to a minimum. In rapidly proliferating epidermis there was a good correlation between change in the number of labelled cells and cells with S phase DNA content. This shows that micro‐flow fluorometry is a rapid method for the study of cell kinetics in a perturbed cell system in vivo.

show abstract

“…On the other hand, the significant increase in the phosphorus content in the DNA fraction per wet weight suggested that the skin has not yet returned to normal completely at 10 days. Indeed, the labelling index data in the literature indicates that ^^H-thymidine incorporation returns to normal with a synchronous wave, but was still raised slightly at 9 or 10 days after stripping (Schellander, 1969;Astley & Levine, 1974). However, it was not probable that the increase in proliferation was maximal at 10 days, even though the disappearance of hyperplasia could be explained by increased proliferation simultaneous with rapid keratinization after mechanical stimulation (Bertsch ei al., 1976).…”

Section: Drscussronmentioning

confidence: 99%

Studies on the regeneration of the skin barrier and the changes in 32P incorporation into the epidermis after stripping

Komatsu

Suzuki

1982

British Journal of Dermatology

View full text Add to dashboard Cite

The epidermis of the rabbit ear was stripped and the effects on percutaneous absorption and 32P incorporation into lipid, TCA-soluble, RNA and DNA fractions in the epidermis was studied for 21 days. Skin penetration by 14C-propylene glycol increased 300-fold immediately after stripping twenty times. Three to five days after stripping, penetration had returned almost to normal levels, but it required several additional days for the barrier function to become normal. The process of regeneration of the skin barrier paralleled the morphological changes in the epidermis but was inconsistent with the biochemical data. A significant increase in 32P incorporation was observed at 10 days, when the hyperplasia had disappeared and percutaneous absorption had returned to normal. The discrepancy between 32P incorporation and morphology or percutaneous absorption after stripping is discussed.

show abstract

Reaktion von Epidermis und subepidermalem Bindegewebe auf Hornschichtabrisse

Cited by 15 publications

References 6 publications

Epidermal Regeneration After Cellophane Tape Stripping of Hairless Mouse Skin

Epidermal Regeneration After Cellophane Tape Stripping of Hairless Mouse Skin

Regenerative Proliferation of Mouse Epidermal Cells Following Adhesive Tape Stripping

Studies on the regeneration of the skin barrier and the changes in 32P incorporation into the epidermis after stripping

Contact Info

Product

Resources

About