Reaktionsfähige hochpolymere auf polystyrolbasis als träger von proteinen und enzymen

Abstract

ZUSAMMENFASSUNG:Durch SUMMARY:Reactive, hydrophilic, insoluble polymers which contain nitrofluorobenzenesulfofonyl groups were prepared by FRIEDEL-CRAFTS reaction of 4-fluorobenzenesulfonyl chloride with polystyrene and subsequent sulfonation and nitration.Reactive, hydrophilic, insoluble polymers containing dinitrofluorophenyl groups were prepared by nitration of copolymers obtained from 4-or 3-fluorostyrene, methacrylic acid, and divinylbenzene.The chemical reactivity of these polymers especially their ability to bind proteins were investigated. The reaction of these polymers with diastase and papain gave enzyme resins; their properties were investigated.Die Untersuchungen uber reaktionsfahige Tragersubstanzen fur physiologisch wichtige Proteine hatten zu der Entwicklung nitrierter Copolymerisate aus ungesattigten Carbonsauren und Methacrylsaure-rnfluoranilid 1) gefuhrt. Die gute Reaktionsfahigkeit und das vergleichsweise hohe Proteinbindungsvermogen dieser Tragersubstanzen beruhten

Covalent Enzyme Immobilization

“…The use of synthetic polymeric carriers bearing halogen atoms can be dated back to the beginning of the 1960s [266,267]. These synthetic polymeric carriers bearing halogen atoms were synthesized from monomers bearing fluoro groups, for example I, II, III, and IV in Scheme 3.58, with co-monomers such as acrylic acid or divinylbenzene.…”

“…Following this work, other synthetic polymers, for example a copolymer of methacrylic acid m-fluoroanilide with methacrylic acid, divinylbenzene or fluorostyrene, have been synthesized since the 1960s [266][267][268][269]. In general, there has been almost no further interest in this type of polymer since the 1970s.…”

Preparation d'invertase insolubilisée par fixation sur bentonite

“…De nombreuses enzymes ont 6th fixees sur divers supports insolubles [l] : polym&res d'acides amin& 123, polymeres synthetiques [3,4] , membranes au collodion [5], derives de la cellulose [6-81, Stphadex [9], verre poreux [lo, 111. L'invertase a deja Btb insolubilisee par fucation sur copolymere nitrl d'acide metacrylique et de m-fluoroanilide d'acide metacrylique [4] ainsi que sur DEAE-cellulose [8] .…”

“…L'invertase a deja Btb insolubilisee par fucation sur copolymere nitrl d'acide metacrylique et de m-fluoroanilide d'acide metacrylique [4] ainsi que sur DEAE-cellulose [8] .…”

Proceedings of The Biochemical Society

“…The following derivatives of synthetic polymers were reacted with proteins: polymeric carboxylic or sulphonic acid chlorides of ion exchange resins, diazotized poly-aminostyrene, poly-isocyanatostyrene, poly-isothiocyanatostyrene (Manecke & Gunzel, 1967), copolymers of N-carboxylic acid anhydrides of L-leucine and p-amino-DL-phenylalanine as diazonium salt (Cebra, Givol, Silman & Katchalski, 1961), diazotized polymers to react copolymers of N-carboxytyrosine anhydride with trypsin , nitrated copolymers of methacrylic acid, methacryl-3fluoroanilide and divinylbenzene, nitrated copolymers of methacrylic acid, 2-, 3-or 4-fluorostyrene and divinylbenzene (Manecke & Forster, 1966), reaction products of polystyrene with 4-fluorobenzenesulphonyl chloride, subsequently sulphonated and nitrated (Manecke & Forster, 1966). Starting from reactive monomers of m-isothiocyanatostyrene (Manecke & Gunzel, 1967) and vinylisothiocyanate (Manecke & Giinzel, unpublished work) are the only examples which were polymerized to yield polymers reactive with proteins (for other references see Manecke, 1962).…”