Real-time analysis of antibody interactions with whole enveloped human cytomegalovirus using surface plasmon resonance

Chenail, Gregg; Brown, Nathan E.; Shea, Ashley; Feire, Adam L.; Deng, Gejing

doi:10.1016/j.ab.2010.12.017

Cited by 14 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vectors (0.2 l of 10 12 to 10 13 pp/ml) were immobilized on a glass prism as described before (33). A dose-dependent binding study was performed using several dilutions of hu-G3BP (10 nM, 20 nM, and 40 nM) with PBS as a running buffer, and then the rAAV surface was regenerated by injection of 100 l of 25 mM NaOH (8). In order to determine the kinetic constants, SPRi data were analyzed with BiaEvaluation software (Biacore) by using a 1:1 Langmuir binding model.…”

Section: Methodsmentioning

confidence: 99%

Human Galectin 3 Binding Protein Interacts with Recombinant Adeno-Associated Virus Type 6

Denard

Beley

Kotin

et al. 2012

J Virol

View full text Add to dashboard Cite

i Recombinant adeno-associated viruses (rAAVs) hold enormous potential for human gene therapy. Despite the well-established safety and efficacy of rAAVs for in vivo gene transfer, there is still little information concerning the fate of vectors in blood following systemic delivery. We screened for serum proteins interacting with different AAV serotypes in humans, macaques, dogs, and mice. We report that serotypes rAAV-1, -5, and -6 but not serotypes rAAV-2, -7, -8, -9, and -10 interact in human sera with galectin 3 binding protein (hu-G3BP), a soluble scavenger receptor. Among the three serotypes, rAAV-6 has the most important capacities for binding to G3BP. rAAV-6 also bound G3BP in dog sera but not in macaque and mouse sera. In mice, rAAV-6 interacted with another protein of the innate immune system, C-reactive protein (CRP). Furthermore, interaction of hu-G3BP with rAAV-6 led to the formation of aggregates and hampered transduction when the two were codelivered into the mouse. Based on these data, we propose that species-specific interactions of AAVs with blood proteins may differentially impact vector distribution and efficacy in different animal models.T he recombinant adeno-associated vector (rAAV) platform, derived from a nonpathogenic dependovirus, has many attributes suitable for in vivo gene transfer: rAAV vectors are capable of transducing a wide range of cell types, including dividing and nondividing cells; rAAV genomes persist as episomal chromatin in the nucleus of transduced cells (38); and stable, persistent expression has been reported for many transgenes in different tissues and species (6,12,36,39). rAAVs have proven to be efficient in preclinical studies in animal models (16,28), and results from clinical trials are promising (7,47).In the case of systemic diseases, clinical relevance requires widespread distribution of the vector in order to target entire organs. This is particularly true for myopathies, where all striated muscles of the skeletal musculature and, frequently, cardiac muscles have to be treated. In this case, vascular delivery would be the optimal route for rAAV administration. Intravascular injection of a number of rAAV serotypes has proven efficient in murine models of muscular dystrophies (11,17,18,34,35). However, translating this approach to large animal models and humans is still challenging. Acquired immunity and neutralizing antibodies present in a large fraction of the human population might obviously be restrictive for rAAV gene delivery (5,20,27,29,30). Moreover, recent studies have demonstrated that serum might also contain other factors neutralizing rAAV vectors (40), indicating that detailed characterization of rAAV's molecular interactions in the bloodstream is obviously important in order to improve vector efficacy.We looked for serum proteins, other than immunoglobulins, which could interact with rAAVs in the bloodstreams of different species. By using a multidisciplinary approach involving proteomics, binding assays, electron microscopy (EM), and in vivo stud...

show abstract

Section: Methodsmentioning

confidence: 99%

Human Galectin 3 Binding Protein Interacts with Recombinant Adeno-Associated Virus Type 6

Denard

Beley

Kotin

et al. 2012

J Virol

View full text Add to dashboard Cite

show abstract

“…Results have established the protocol, where the interaction of entire viral particles having dimension of 120 nm with mAb could be monitored. Since then many plant and animal viruses have been analyzed using SPR systems (Hardy and Dimmock, 2003;Boltovets et al, 2004;Baac et al, 2006;Nilsson et al, 2010;Wang et al, 2010;Chenail et al, 2011;Yakes et al, 2013). Another study reported quantification of influenza virus based on inhibition of HA antibody binding (Nilsson et al, 2010).…”

Section: Monitoring Intact Viruses Using An Antibody As a Bioreceptormentioning

confidence: 99%

Surface Plasmon Resonance: A Boon for Viral Diagnostics

Singh

2017

Reference Module in Life Sciences

View full text Add to dashboard Cite

“…Most viruses are smaller than the current limit for SPR measurements (approximately 300 nm); thus, it is possible that many plant and animal viruses can be analyzed. With these developments, for the past two decades, a few viruses have been detected using the protocol described above, the SPR platform [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ], with some modifications, and these viruses are cataloged in Table 1 .…”

Section: Monitoring Intact Viruses Using An Antibody As a Biorecepmentioning

confidence: 99%

Monitoring Intact Viruses Using Aptamers

Kumar

2016

Biosensors

View full text Add to dashboard Cite

Viral diagnosis and surveillance are necessary steps in containing the spread of viral diseases, and they help in the deployment of appropriate therapeutic interventions. In the past, the commonly employed viral detection methods were either cell-culture or molecule-level assays. Most of these assays are laborious and expensive, require special facilities, and provide a slow diagnosis. To circumvent these limitations, biosensor-based approaches are becoming attractive, especially after the successful commercialization of glucose and other biosensors. In the present article, I have reviewed the current progress using the biosensor approach for detecting intact viruses. At the time of writing this review, three types of bioreceptor surfaces (antibody-, glycan-, and aptamer-based) have been explored on different sensing platforms for detecting intact viruses. Among these bioreceptors, aptamer-based sensors have been increasingly explored for detecting intact viruses using surface plasmon resonance (SPR) and other platforms. Special emphasis is placed on the aptamer-based SPR platform in the present review.

show abstract

Real-time analysis of antibody interactions with whole enveloped human cytomegalovirus using surface plasmon resonance

Cited by 14 publications

References 11 publications

Human Galectin 3 Binding Protein Interacts with Recombinant Adeno-Associated Virus Type 6

Human Galectin 3 Binding Protein Interacts with Recombinant Adeno-Associated Virus Type 6

Surface Plasmon Resonance: A Boon for Viral Diagnostics

Monitoring Intact Viruses Using Aptamers

Contact Info

Product

Resources

About