Real-Time Cell Analysis of the Cytotoxicity of &lt;i&gt;Origanum acutidens&lt;/i&gt; Essential Oil on HT-29 and HeLa Cell Lines

Altuntas, Feyza Oke; Demirtaş, İbrahim

doi:10.4274/tjps.40427

Cited by 12 publications

(7 citation statements)

References 16 publications

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“…A previous study points out that a carvacrol rich essential oil of origanumacutidens has also significant anti-proliferative effect against the HT-29 cell line. The most active concentration of this oil has been found as 100 μg/mL [17]. Moreover, similar to our results, Kai Fana et al have studied the antiproliferative effect of carvacrol on HCT-116 and they detected the IC 50 dose as 544.4 µM which is an even higher concentration comparing to our result [11].…”

Section: Discussionsupporting

confidence: 89%

Carvacrol Alters Soluble Factors in HCT-116 and HT-29 Cell Lines

et al. 2020

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Background/aim: Natural products are popular insights for researchers to investigate promising anti-cancer agents since some of these substances have lesser adverse effects restricting the treatment than traditional chemotherapeutic agents. A well-known monoterpene Carvacrol, widely consumed in Mediterranean cuisine and lower risks of cancer, has efficient anticancer effects. However, the mechanism of action is yet to be discovered. Materials and methods: The investigation aims to illuminate a new perceptive in the role of this substance on colorectal cancer treatment, by the means of differences in a well-defined range of soluble factors. Carvacrol effect on both HT-29 and HCT-116 cell lines was evaluated on proliferation and the IC 50 values were calculated by the RTCA xCELLigence device. Then MAGPIX assay was performed to obtain the changes in soluble factors of the cell lines. Results: The Multiplexing assay suggests some of these factors were altered in favor of surviving and proliferation in aggressive cell line HCT-116 whereas they were altered against these characters in HT-29, were correlated with the increased IC 50 concentration of HCT-116 in carvacrol treatment. Conclusion: The current study indicates that differences in the levels of these soluble factors could modulate the anticancer effect related to carvacrol.

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Section: Discussionsupporting

confidence: 89%

Carvacrol Alters Soluble Factors in HCT-116 and HT-29 Cell Lines

et al. 2020

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“…Also, extrusion of the drug by cell membrane pumps, increased DNA damage repair, redistribution of intracellular accumulation of drugs, modification of drug target molecules, suppression of drug-induced apoptosis, up-regulation of lipids and other biochemical changes will be the others reasons 32 . Essential oil of Origanum acutidens demonstrated strong cytotoxic effect in HeLa cells [inhibitor concentration (IC50) <10 µg/ml] using the method xCELLigence 55 . O. majorana essential oil's and its oxygenated monoterpene component linalool were found more cytotoxic in parental H1299 cells than epirubicin-HCl resistant H1299 cells 22 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells

Erdoğan¹,

Özkan²,

Ünal³

et al. 2018

Cukurova Medical Journal

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ÖzPurpose: In this study, we evaluated Sideritis stricta (S. stricta), as potential-oxidative agents against parental and epirubicin-HCl resistant H1299 cells. Material and Methods: Oxidative stress biomarkers such as malondialdehyde level determined in cell lysates. Assessment of cell viability was made by CellTiter-Blue® Cell Viability Assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5diphenyl-2H-tetrazoliumbromide (MTT) assay after 20-600 μg/mL essential oil concentrations treated to parental and epirubicin-HCl (drug) resistant H1299 cells for 24, 48 and 72 h. Malondialdehyde levels were assayed for determining the membrane damaging effects. Results: Parental H1299 cells were found to be more sensitive to cytotoxic effect of the essential oil. Essential oil showed cytotoxic and more selective effects depend on time and concentration. Essential oil caused increasing malondialdehyde level on both parental and drug resistant H1299 cells. The highest concentration of the essential oil (IC70) treatment caused the highest membrane damage on both parental and drug resistant H1299 cells. Conclusions: Parental and epirubicin-HCl resistant H1299 cells showed different cellular response against potential antitumour and pro-oxidative effects of essential oil. Amaç:. Bu çalışmada, Sideritis stricta (S. stricta) parental ve epirubicin-HCl dirençli H1299 hücrelerine karşı potansiyel oksidatif ajan olarak değerlendirilmiştir. Gereç ve Yöntem: Hücre lisatlarında malondialdehit seviyesi gibi oksidatif stres biyobelirteçleri saptanmıştır. Hücre sitotoksisitesinin değerlendirilmesi, 20-600 μg/mL konsantrasyonlarında 24, 48 ve 72 saat parental ve epirubicin-HCl (ilaç) dirençli H1299 hücrelerine uçucu yağ uygulamasından sonra CellTiter-Blue® Hücre Canlılık Testi ve 3-(4,5-dimetil-2-tiazolil) -2,5-difenil-2Htetrazolyumbromid (MTT) testi ile yapılmıştır. Malondialdehid seviyeleri membran hasar etkisini ortaya koymak için belirlenmiştir. Bulgular:Parental H1299 hücreleri uçucu yağın sitotoksik etkisine karşı daha duyarlı bulunmuştur. Uçucu yağ, zaman ve konsantrasyona bağlı olarak sitotoksik ve daha seçici etkiler göstermiştir. Uçucu yağ, hem parental hem de ilaca dirençli H1299 hücrelerinde malondialdehit seviyesinde artışa neden olmuştur. Uçucu yağın (IC70) en yüksek konsantrasyonu, hem parental hem de ilaca dirençli H1299 hücrelerinde en yüksek membran hasarına neden olmuştur. Sonuç: Parental ve epirubicin-HCl dirençli H1299 hücreleri uçucu yağın potansiyel antitümör ve pro-oksidatif etkilerine karşı farklı hücresel tepkiler göstermiştir.

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“…Treatment with EOs of Dittrichia viscosa, Hedychium coronarium, and Rosmarinus officinalis with monoterpenoid 1,8-cineole as the major constituent reported cytotoxicity effects to HeLa cells [63,113,114]. Lavandula pubescens, Origanum acutidens, and Satureja boissieri EOs contain high amounts of carvacrol, a monoterpenoid compound [115][116][117]. These EOs exhibited a significant cytotoxic effect against HeLa cell lines at tested concentrations.…”

Section: Essential Oils With High Monoterpenoidsmentioning

confidence: 99%

Essential Oils in Cervical Cancer: Narrative Review on Current Insights and Future Prospects

Abd Rashid,

Mohamad Najib,

Abdul Jalil

et al. 2023

Antioxidants

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Cervical cancer is a prevalent and often devastating disease affecting women worldwide. Traditional treatment modalities such as surgery, chemotherapy, and radiation therapy have significantly improved survival rates, but they are often accompanied by side effects and challenges that can impact a patient’s quality of life. In recent years, the integration of essential oils into the management of cervical cancer has gained attention. This review provides an in-depth exploration of the role of various essential oils in cervical cancer, offering insights into their potential benefits and the existing body of research. The review also delves into future directions and challenges in this emerging field, emphasizing promising research areas and advanced delivery systems. The encapsulation of essential oils with solid lipid nanoparticles, nanoemulsification of essential oils, or the combination of essential oils with conventional treatments showed promising results by increasing the anticancer properties of essential oils. As the use of essential oils in cervical cancer treatment or management evolves, this review aims to provide a comprehensive perspective, balancing the potential of these natural remedies with the challenges and considerations that need to be addressed.

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Real-Time Cell Analysis of the Cytotoxicity of <i>Origanum acutidens</i> Essential Oil on HT-29 and HeLa Cell Lines

Cited by 12 publications

References 16 publications

Carvacrol Alters Soluble Factors in HCT-116 and HT-29 Cell Lines

Carvacrol Alters Soluble Factors in HCT-116 and HT-29 Cell Lines

Evaluation of the cytotoxic and membrane damaging effects of mountain tea (Sideritis stricta Boiss & Heldr.) essential oil on parental and epirubicin-HCl resistant H1299 cells

Essential Oils in Cervical Cancer: Narrative Review on Current Insights and Future Prospects

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