Real-time quantitative phase imaging based on transport of intensity equation with dual simultaneously recorded field of view

Tian, Xiaolin; Yu, Wei; Meng, Xin; Sun, Aihui; Li, Xue; Liu, Cheng; Wang, Shouyu

doi:10.1364/ol.41.001427

Cited by 71 publications

(32 citation statements)

References 33 publications

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“…Interference based methods composed of digital holography (Miccio et al ., ; Kim & Park, ; Bianco et al ., ; Memmolo et al ., ; Bianco et al ., ; Kim et al ., ; Merola et al ., ; Zhang et al ., ), interferometric microscopy (Wang et al ., ; Lee & Park, ; Wang et al ., ,b; Wang et al ., ) and spatial light interference microscopy (Babacan et al ., ; Majeed et al ., ) can obtain cellular phase distributions with high speed and accuracy, while the extra reference causes complex optical setup, and it is also difficult to be integrated into commercial microscopes, thus they are not widely adopted in biological and medical fields. Compared to these iterative or interference methods, quantitative phase imaging based on transport of intensity equation (TIE) method shows its potentials on cellular phase observations and measurements (Kou et al ., ; Waller et al ., ; Waller et al ., ; Tian et al ., ; Zhu et al ., ; Tian et al ., ; Yu et al ., ,b; Meng et al ., ). With single in‐focus and another two symmetric defocus images, sample phase distributions can be computed via solving Poisson equation.…”

Section: Introductionmentioning

confidence: 98%

“…Detailed procedure of rapid in-focus correction on quantitative amplitude and phase imaging according to TIE. et al, 2011;Tian et al, 2012;Zhu et al, 2014;Tian et al, 2016b;Yu et al, 2016a,b;Meng et al, 2017). With single in-focus and another two symmetric defocus images, sample phase distributions can be computed via solving Poisson equation.…”

Section: Introductionmentioning

confidence: 99%

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Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method

Meng

Tian

Kong

et al. 2017

Journal of Microscopy

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Transport of intensity equation (TIE) method can acquire sample phase distributions with high speed and accuracy, offering another perspective for cellular observations and measurements. However, caused by incorrect focal plane determination, blurs and halos are induced, decreasing resolution and accuracy in both retrieved amplitude and phase information. In order to obtain high-accurate sample details, we propose TIE based in-focus correction technique for quantitative amplitude and phase imaging, which can locate focal plane and then retrieve both in-focus intensity and phase distributions combining with numerical wavefront extraction and propagation as well as physical image recorder translation. Certified by both numerical simulations and practical measurements, it is believed the proposed method not only captures high-accurate in-focus sample information, but also provides a potential way for fast autofocusing in microscopic system.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method

Meng

Tian

Kong

et al. 2017

Journal of Microscopy

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“…Noninterferometric imaging techniques, such as those based on the transport of intensity equation (TIE), have been proposed for quantitative phase imaging, [14][15][16][17][18][19][20][21][22] which does not require a reference beam and hence makes the system simple and compact. Teague proposed the TIE from the Helmholtz equation under the paraxial approximation with the assumption of a monochromatic and coherent beam.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional fluorescence imaging using the transport of intensity equation

et al. 2019

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We propose a nonscanning three-dimensional (3-D) fluorescence imaging technique using the transport of intensity equation (TIE) and free-space Fresnel propagation. In this imaging technique, a phase distribution corresponding to defocused fluorescence images with a point-light-source-like shape is retrieved by a TIE-based phase retrieval algorithm. From the obtained phase distribution, and its corresponding amplitude distribution, of the defocused fluorescence image, various images at different distances can be reconstructed at the desired plane after Fresnel propagation of the complex wave function. Through the proposed imaging approach, the 3-D fluorescence imaging can be performed in multiple planes. The fluorescence intensity images are captured with the help of an electrically tunable lens; hence, the imaging technique is free from motion artifacts. We present experimental results corresponding to microbeads and a biological sample to demonstrate the proposed 3-D fluorescence imaging technique.

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“…In digital holography and other interference based imaging methods, the regular reference beam adopted always makes the setup complicated and sensitive to the outside disturbance [3][4][5]. Transport of intensity equation (TIE) method, which is a non-interference phase imaging technique, has much simpler setup and lower requirement on the working environment [6][7][8][9][10][11][12][13], and recently developed Fourier ptychographic microscopy [14,15] is another non-interference phase imaging technique that can provide complex amplitude of sample, however like other lens aided imaging methods, the accuracy of these two techniques is dependent to the used optics.…”

Section: Introductionmentioning

confidence: 99%

Ultra-high speed digital micro-mirror device based ptychographic iterative engine method

Sun¹,

He²,

Kong³

et al. 2017

Biomed. Opt. Express

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Abstract:To reduce the long data acquisition time of the common mechanical scanning based Ptychographic Iterative Engine (PIE) technique, the digital micro-mirror device (DMD) is used to form the fast scanning illumination on the sample. Since the transverse mechanical scanning in the common PIE is replaced by the on/off switching of the micromirrors, the data acquisition time can be reduced from more than 15 minutes to less than 20 seconds for recording 12 × 10 diffraction patterns to cover the same field of 147.08 mm 2 . Furthermore, since the precision of DMD fabricated with the optical lithography is always higher than 10 nm (1 μm for the mechanical translation stage), the time consuming positionerror-correction procedure is not required in the iterative reconstruction. These two improvements fundamentally speed up both the data acquisition and the reconstruction procedures in PIE, and relax its requirements on the stability of the imaging system, therefore remarkably improve its applicability for many practices. It is demonstrated experimentally with both USAF resolution target and biological sample that, the spatial resolution of 5.52 μm and the field of view of 147.08 mm 2 can be reached with the DMD based PIE method. In a word, by using the DMD to replace the translation stage, we can effectively overcome the main shortcomings of common PIE related to the mechanical scanning, while keeping its advantages on both the high resolution and large field of view.

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Real-time quantitative phase imaging based on transport of intensity equation with dual simultaneously recorded field of view

Cited by 71 publications

References 33 publications

Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method

Rapid in‐focus corrections on quantitative amplitude and phase imaging using transport of intensity equation method

Three-dimensional fluorescence imaging using the transport of intensity equation

Ultra-high speed digital micro-mirror device based ptychographic iterative engine method

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