Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds

Zhang, Yongjiang; Zhao, Wenran; Li, Mingfu; Chen, Hongjun; Zhu, Shuifang; Fan, Zaifeng

doi:10.1016/j.jviromet.2010.11.002

Cited by 54 publications

(53 citation statements)

References 16 publications

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“…MCMV often induces corn lethal necrosis (CLN) resulting from synergistic interaction between this virus and maize dwarf mosaic virus (MDMV) (Niblett and Clafin, 1978;Goldberg and Brakke, 1987), wheat streak mosaic virus (WSMV) (Scheets, 1998), or sugarcane mosaic potyvirus (SCMV) (Uyemoto et al, 1980), leading to serious yield losses in corn (Uyemoto, 1983;Scheets, 1998;Morales et al, 1999;Xie et al, 2011). Adults of six species of chrysomelid beetle were reported to transmit MCMV under experimental conditions (Nault et al, 1978), and seed transmission was also reported for the virus (Jensen et al, 1991;Zhang et al, 2011). Recently, Dr. Bressan found thrips transmitting MCMV in Hawaii, USA (personal communication, Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA).…”

Section: Introductionmentioning

confidence: 99%

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Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Wang

Huan

et al. 2013

J. Zhejiang Univ. Sci. B

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Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327 680, 1:64 000, and 1:3 276 800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.

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Section: Introductionmentioning

confidence: 99%

“…At present, several methods have been developed for detection of MCMV (Uyemoto, 1983;Morales et al, 1999;Stenger et al, 2007;Stenger and French, 2008;Zhang et al, 2011). Among these detection methods, serological methods are more frequently used to detect large numbers of samples in field surveys.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Wang

Huan

et al. 2013

J. Zhejiang Univ. Sci. B

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“…MCMV is normally transmitted by thrips and beetles while SCMV is transmitted by aphids (CGIAR, 2012;Kiruwa et al, 2016). The viruses can as well be transmitted from one generation to another through seed which are infected, this enhance the possibility for wide spread of MLND (Zhang et al, 2011). Infected plants show mild to severe mottling on the leaves (Gowda et al, 2015;De Groote et al, 2016;Kagoda et al, 2016), usually starting from the base of young leaves in the whorl and extending upwards toward the leaf tips (Wangai et al, 2012b).…”

Section: Maize Lethal Necrosis Disease (Mlnd)mentioning

confidence: 99%

Genetic diversity of maize accessions for maizelethal necrosis disease resistance

Ndakidemi

Sospeter

Ndunguru

et al. 2016

IJARe

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Maize is among the most preferred crop in Tanzania and other parts of the world. However, its production has been facing a number of challenges. Maize Lethal Necrosis Disease (MLND) is a new challenge in Eastern Africa. The control of MLND is said to be complicated as it is caused by a combination of more than one virus viz. Maize Chlorotic Mottle Virus (MCMV) and Sugarcane Mosaic Virus (SCMV). Stakeholders agree that the priority is to identify MLND resistant maize varieties. Genetic diversity provides the source of traits required against maize production challenges such as MLND. The study of genetic diversity in maize accessions often involves characterizing morphological plant characteristics as well as molecular marker techniques to study variation at DNA level. This review explores different literatures that address the importance of genetic diversity and the possibility of generating information towards obtaining potential materials against maize challenges and MLND in particular.

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“…For that, we are now performing experiments including infection of quarantine viruses into seeds of various host plants and developing techniques to detect viruses in seeds with high sensitivity and simplicity. A previous study demonstrated real time RT-PCR based approach could detect Maize chlorotic mottle virus in maize seeds (Zhang et al, 2011).…”

mentioning

confidence: 99%

“…In addition, real-time PCR assay is recently regarded as the next favorable technique because of its high sensitivity (Harper et al, 2011;Zhang et al, 2011). A previous study has shown that 4 fg of total RNA or 25 copies of RNA transcripts are high enough to detect virus and sensitivity of this method seems to be ten-fold higher than other RT-PCR gel electrophoresis approach (Zhang et al, 2011). Therefore, it could be of interest to develop detection system based on real time RT-PCR in near future.…”

mentioning

confidence: 99%

RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses

Lee¹,

Cho²,

Choi³

et al. 2011

The Plant Pathology Journal

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In order to detect quarantine plant viruses, we developed reverse transcription-polymerase chain reaction (RT-PCR) primer pairs for five single-stranded (ss) plant RNA viruses that are not currently reported in Korea but could be potential harmful plant viral pathogens. Three viruses such as Chilli veinal mottle virus (ChiVMV), Colombian datura virus (CDV), and Tobacco etch virus (TEV) belong to the genus Potyvirus while Chrysanthemum stem necrosis virus (CSNV) and Iris yellow spot virus (IYSV) are members of the genus Tospovirus. To design RT-PCR primers, we used reported gene sequences corresponding to the capsid protein and polyprotein for ChiVMV, CDV, and TEV while using nucleocapsid protein regions for CSNV and IYSV. At least two different primer pairs were designed for each virus. Fifteen out of 16 primer pairs were successfully applied in detection of individual quarantine virus with high specificity and efficiency. Taken together, this study provides a rapid and useful protocol for detection of five quarantine viruses.

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Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds

Cited by 54 publications

References 16 publications

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Genetic diversity of maize accessions for maizelethal necrosis disease resistance

RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses

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