Recent advances in FRET-Based biosensors for biomedical applications

Imani, Mahsa; Mohajeri, Nasrin; Rastegar, Mojgan; Zarghami, Nosratollah

doi:10.1016/j.ab.2021.114323

Cited by 63 publications

(33 citation statements)

References 119 publications

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“…A sensitive Abz-peptidyl-pNA fluorescence resonance energy transfer (FRET) protocol can quantify the activity of HtrA inhibitors using an in vitro assay ( Fig. 7 B) [16] , [75] , [76] . The quenched fluorescent peptide substrate comprises Abz (fluorogenic group), peptides with cleavage sites, and pNA (P-nitroaniline), a known fluorescence quencher.…”

Section: Drug Development Targeting Htra Proteinsmentioning

confidence: 99%

Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view

Song

Kang

et al. 2022

Computational and Structural Biotechnology Journal

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Members of the high temperature requirement A (HtrA) protein family are widely distributed amongst prokaryotic and eukaryotic species. HtrA proteins have ATP-independent dual chaperone-protease activity and mediate protein quality control. Emerging evidence indicates that HtrA family members are vital for establishing infections and bacterial survival under stress conditions. Bacterial HtrA proteins are increasingly thought of as important new targets for antibacterial drug development. Recent literature suggests that HtrA protein AlgW from Pseudomonas aeruginosa has distinct structural, functional, and regulatory characteristics. The novel dual-signal activation mechanism seen in AlgW is required to modulate stress and drug responses in bacteria, prompting us to review our understanding of the many HtrA proteins found in microorganisms. Here, we describe the distribution of HtrA gene orthologues in pathogenic bacteria, discuss their structure–function relationships, outline the molecular mechanisms exhibited by different bacterial HtrA proteins in bacteria under selective pressure, and review the significance of recently developed small molecule inhibitors targeting HtrA in pathogenic bacteria.

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Section: Drug Development Targeting Htra Proteinsmentioning

confidence: 99%

Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view

Song

Kang

et al. 2022

Computational and Structural Biotechnology Journal

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“…Förster resonance energy transfer (FRET) microscopy is a feasible approach to measure WWOX function in a real-time manner. FRET can be utilized to determine spatial proximity among proteins either at a single or multiple protein levels in cultured cells in a real-time mode [ 18 , 43 , 44 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. For example, binding of a CFP (cyan fluorescence protein)-tagged bait (or donor) protein with a YFP (yellow fluorescence protein)-tagged target (or acceptor) protein results in energy flow from the donor to the acceptor.…”

Section: Wwox Functional Measurement By Time-lapse Fret Microscopymentioning

confidence: 99%

WWOX Controls Cell Survival, Immune Response and Disease Progression by pY33 to pS14 Transition to Alternate Signaling Partners

Liu

Nagarajan

Chiang

et al. 2022

Cells

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Tumor suppressor WWOX inhibits cancer growth and retards Alzheimer’s disease (AD) progression. Supporting evidence shows that the more strongly WWOX binds intracellular protein partners, the weaker is cancer cell growth in vivo. Whether this correlates with retardation of AD progression is unknown. Two functional forms of WWOX exhibit opposite functions. pY33-WWOX is proapoptotic and anticancer, and is essential for maintaining normal physiology. In contrast, pS14-WWOX is accumulated in the lesions of cancers and AD brains, and suppression of WWOX phosphorylation at S14 by a short peptide Zfra abolishes cancer growth and retardation of AD progression. In parallel, synthetic Zfra4-10 or WWOX7-21 peptide strengthens the binding of endogenous WWOX with intracellular protein partners leading to cancer suppression. Indeed, Zfra4-10 is potent in restoring memory loss in triple transgenic mice for AD (3xTg) by blocking the aggregation of amyloid beta 42 (Aβ42), enhancing degradation of aggregated proteins, and inhibiting activation of inflammatory NF-κB. In light of the findings, Zfra4-10-mediated suppression of cancer and AD is due, in part, to an enhanced binding of endogenous WWOX and its binding partners. In this perspective review article, we detail the molecular action of WWOX in the HYAL-2/WWOX/SMAD4 signaling for biological effects, and discuss WWOX phosphorylation forms in interacting with binding partners, leading to suppression of cancer growth and retardation of AD progression.

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“…Therefore, artificial chemical systems would contain several symmetrically distributed light-harvesting donor-acceptor sites (into micellar, vesicular, or dendrimer structures) to appease a directional energy transfer over nanoscale dimensions. In recent years, the fluorescence (or Förster) resonance energy-transfer (FRET) mechanism has been studied in-depth to concentrate absorbed energy at a spatially confined site in nanometric regions [85][86][87]. FRET systems involve the presence of a significant amount of chromophores able to mainly absorb light radiation, e.g., in the visible region of the spectrum (this is addressed in more detail below).…”

Section: Photo-induced Energy Transfer Processesmentioning

confidence: 99%

Chromophoric Dendrimer-Based Materials: An Overview of Holistic-Integrated Molecular Systems for Fluorescence Resonance Energy Transfer (FRET) Phenomenon

et al. 2021

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Dendrimers (from the Greek dendros → tree; meros → part) are macromolecules with well-defined three-dimensional and tree-like structures. Remarkably, this hyperbranched architecture is one of the most ubiquitous, prolific, and recognizable natural patterns observed in nature. The rational design and the synthesis of highly functionalized architectures have been motivated by the need to mimic synthetic and natural-light-induced energy processes. Dendrimers offer an attractive material scaffold to generate innovative, technological, and functional materials because they provide a high amount of peripherally functional groups and void nanoreservoirs. Therefore, dendrimers emerge as excellent candidates since they can play a highly relevant role as unimolecular reactors at the nanoscale, acting as versatile and sophisticated entities. In particular, they can play a key role in the properties of light-energy harvesting and non-radiative energy transfer, allowing them to function as a whole unit. Remarkably, it is possible to promote the occurrence of the FRET phenomenon to concentrate the absorbed energy in photoactive centers. Finally, we think an in-depth understanding of this mechanism allows for diverse and prolific technological applications, such as imaging, biomedical therapy, and the conversion and storage of light energy, among others.

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Recent advances in FRET-Based biosensors for biomedical applications

Cited by 63 publications

References 119 publications

Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view

Function, molecular mechanisms, and therapeutic potential of bacterial HtrA proteins: An evolving view

WWOX Controls Cell Survival, Immune Response and Disease Progression by pY33 to pS14 Transition to Alternate Signaling Partners

Chromophoric Dendrimer-Based Materials: An Overview of Holistic-Integrated Molecular Systems for Fluorescence Resonance Energy Transfer (FRET) Phenomenon

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