“…A number of cultures and organisms can perform MICP, with varying degrees of calcium carbonate mineralization efficiency, and include autotrophs such as Cyanobacteria, Synechococcus , and Prochlorococcus and heterotrophs such as Sporosarcina pasteurii ( Bacillus sphaericus ), B. megaterium, B. subtilis, B . cereus, B. cohnii, B. pseudofirmus, B. alkalinitrilicus, Diaphorobacter nitroreducens, Pseudomonas aeruginosa, Desulfovibrio brasiliensis, Desulfovibrio vilgaris, B. mucilaginous [10, 6]. Among this wide spectrum of axenic and non-axenic cultures that can produce carbonate ions and precipitate calcium carbonate in alkaline conditions, Lysinibacillus sphaericus , formerly known as B. sphaericus , has abilities that make this species a favorable biological component in MICP activities such as: forming long-lasting spores even more than 50 years in environmentally harsh conditions [6], creating rhombohedral and tightly crystal-packed layers, having high rates of urea hydrolysis and calcium carbonate precipitation, and precipitating bio-minerals based on both urea hydrolysis and denitrification pathways [2, 11, 12, 13, 14, 5].…”