Recent advances of metabolic engineering strategies in natural isoprenoid production using cell factories

Li, Meijie; Hou, Feifei; Wu, Tong; Jiang, Xinglin; Li, Fuli; Liu, Haobao; Xian, Ming; Zhang, Haibo

doi:10.1039/c9np00016j

Cited by 101 publications

(85 citation statements)

References 142 publications

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“…3). More generally, the MVA pathway is germane to Archaea and present in most eukaryotes, fungi (Saccharomyces cerevisiae), plant cytoplasm, and some bacteria (enterococci, staphylococci, and streptococci); while the MEP pathway is germane to Bacteria and exists in most cyanobacteria, algae, plant chloroplasts, and some protozoa [147][148][149]. Studies revealed that formation of diverse terpenes, including triterpenes (steroids), brassinosteroids, ubiquinones and most sesquiterpenes originates from the MVA pathway, whereas isoprene, hemiterpenes, monoterpenoids, some sesquiterpenes, diterpenoids, tetraterpenoids, abscisic acid, strigolactones, gibberellins, prenylquinones, and the phytol tail of chlorophylls are derived from the MEP pathway [140,150,151].…”

Section: Isoprenoids Biosynthetic Pathwaysmentioning

confidence: 99%

Strategies for the production of biochemicals in bioenergy crops

Lin

Eudes

2020

Biotechnol Biofuels

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Industrial crops are grown to produce goods for manufacturing. Rather than food and feed, they supply raw materials for making biofuels, pharmaceuticals, and specialty chemicals, as well as feedstocks for fabricating fiber, biopolymer, and construction materials. Therefore, such crops offer the potential to reduce our dependency on petrochemicals that currently serve as building blocks for manufacturing the majority of our industrial and consumer products. In this review, we are providing examples of metabolites synthesized in plants that can be used as bio-based platform chemicals for partial replacement of their petroleum-derived counterparts. Plant metabolic engineering approaches aiming at increasing the content of these metabolites in biomass are presented. In particular, we emphasize on recent advances in the manipulation of the shikimate and isoprenoid biosynthetic pathways, both of which being the source of multiple valuable compounds. Implementing and optimizing engineered metabolic pathways for accumulation of coproducts in bioenergy crops may represent a valuable option for enhancing the commercial value of biomass and attaining sustainable lignocellulosic biorefineries.

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Section: Isoprenoids Biosynthetic Pathwaysmentioning

confidence: 99%

Strategies for the production of biochemicals in bioenergy crops

Lin

Eudes

2020

Biotechnol Biofuels

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“…Among the comprehensive manipulations of microbial metabolism by heterogeneous pathways, two strategies have been used to improve the productivity of microbial cell factories. One strategy focuses on the improvement of biosynthesis by key enzyme activity analysis, evolution and modification, [ 5,6 ] pathway construction and optimization, [ 7 ] and metabolic adaptation of the host strain. [ 8 ] The other strategy focuses on the molecular transfer of intermediates and final products from membrane‐enveloped reaction pools.…”

Section: Introductionmentioning

confidence: 99%

Transporter Engineering for Microbial Manufacturing

Zhu

Zhou

Wang

et al. 2020

Biotechnology Journal

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Microbes play an important role in biotransformation and biosynthesis of biofuels, natural products, and polymers. Therefore, microbial manufacturing has been widely used in medicine, industry, and agriculture. However, common strategies including enzyme engineering, pathway optimization, and host engineering are generally inadequate to obtain an efficient microbial production system. Transporter engineering provides an alternative strategy to promote the transmembrane transfer of substrates, intermediates, and final products in microbial cells and thus enhances production by alleviating feedback inhibition and cytotoxicity caused by final products. According to the current studies in transport engineering, native transporters usually have low expression and poor transportation ability, resulting in inefficient transport processes and microbial production. In this review, current approaches for transporter mining, characterization, and verification are comprehensively summarized. Practical approaches to enhance the transport system in engineered cells, such as balancing transporter overexpression and cell growth, and evolution of native transporters are discussed. Furthermore, the applications of transporter engineering in microbial manufacturing, including enhancement of substrate utilization, concentration of metabolic flux to the target pathway, and acceleration of efflux and recovery of products, demonstrate its outstanding advantages and promising prospects.

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“…In addition to those successful approaches, mining and exploiting key metabolic enzymes with high catalytic efficiencies could be an efficient strategy [28,29]. Metabolic flux towards lycopene production could be effectively improved with the use of substitutive key enzymes with higher catalytic activities along with overexpression strategies [7,25,30].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg

et al. 2019

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Microbial production is a promising method that can overcome major limitations in conventional methods of lycopene production, such as low yields and variations in product quality. Significant efforts have been made to improve lycopene production by engineering either the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway or mevalonate (MVA) pathway in microorganisms. To further improve lycopene production, it is critical to utilize metabolic enzymes with high specific activities. Two enzymes, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and farnesyl diphosphate synthase (IspA), are required in lycopene production using MEP pathway. Here, we evaluated the activities of Dxs and IspA of Vibrio sp. dhg, a newly isolated and fast-growing microorganism. Considering that the MEP pathway is closely related to the cell membrane and electron transport chain, the activities of the two enzymes of Vibrio sp. dhg were expected to be higher than the enzymes of Escherichia coli. We found that Dxs and IspA in Vibrio sp. dhg exhibited 1.08-fold and 1.38-fold higher catalytic efficiencies, respectively. Consequently, the heterologous overexpression improved the specific lycopene production by 1.88-fold. Our findings could be widely utilized to enhance production of lycopene and other carotenoids.

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Recent advances of metabolic engineering strategies in natural isoprenoid production using cell factories

Abstract: This review covers the strategies mostly developed in the last three years for microbial production of isoprenoid, classified according to the engineering targets.

Cited by 101 publications

References 142 publications

Strategies for the production of biochemicals in bioenergy crops

Strategies for the production of biochemicals in bioenergy crops

Transporter Engineering for Microbial Manufacturing

Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg

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