Recent applications of mass spectrometry to antibiotic research

Borders, Donald B.; Carter, Guy T.; Hargreaves, Ronald T.; Siegel, Marshall M.

doi:10.1002/mas.1280040303

Cited by 18 publications

(5 citation statements)

References 164 publications

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“…This group of antibiotics consists of macrocyclic lactones glycosylated to the deoxy sugars L-clandisone and D-desosamine with [M+H] + ions above m/z 700 ( Table 6). The loss of of these sugars yields a characteristic product ion for macrolides, [44] as observed for tulathromycin and erythromycin after the loss of clandisone leading to the ions at m/z 577 (#1) and 576 (#4). Similarly, loss of desosamine in tilmicosin led to the formation of the ion at m/z 696 (#3).…”

Section: Macrolidesmentioning

confidence: 94%

Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Geis-Asteggiante

Núñez

Lehotay

2014

Rapid Commun. Mass Spectrom.

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Section: Macrolidesmentioning

confidence: 94%

Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Geis-Asteggiante

Núñez

Lehotay

2014

Rapid Commun. Mass Spectrom.

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“…Most of these compounds have been investigated in earlier studies and there is no useful purpose in discussing their fragmentation patterns (see for example the reviews by Borders et a1. 4 and Casy et a1.7. However, to facilitate this discussion a number of mass spectra as well as the proposed molecular structures of some of the observed major fragment ions are presented.…”

Section: Resultsmentioning

confidence: 92%

Investigation of some β‐lactam antibiotics by means of 2–35 keV cesium ion bombardment

Hamdan¹,

Curcuruto²,

Tarzia³

et al. 1992

Rapid Comm Mass Spectrometry

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A number of commercial B-lactam antibiotics are investigated by means of cesium ion (Cs') bombardment in the kinetic energy range 2-35 keV. The relative intensities of the protonated molecules, as well as some of the major fragment ions, are monitored as a function of the Cs' kinetic energy. The dependence of the intact protonated molecule :fragment ion ratios on the primary-ion kinetic energy is evident in some of the spectra investigated. An attempt is made to interpret these results in terms of existing secondary-ion mass spectrometry data which have been obtained in a different kinetic energy range.The recent increase in the number of commercial mass spectrometers equipped with cesium ion guns which are capable of yielding ion beams of energies as high as 40 keV has raised a number of interesting questions regarding the influence of such relatively high energy on the overall characteristics of the mass spectra generated by these ion beams. Aberth and Burlingame' used 5-30 keV Cs' beams to investigate a number of methyl glucose polysaccharides and bovine insulin, and reported that the integrated ion count signals of the secondary protonated molecules reached their maxima in the energy range 15-20 keV and decreased at higher ion kinetic energies. The effects of the kinetic energy and polyatomicity of a number of primary-ion beams on the secondary-ion yield and the extent of fragmentation have been examined in some detail by Cooks and co-workers.* They reported that the degree of fragmentation observed in the secondary-ion mass spectrometry (SIMS) spectra of some organic salts was roughly proportional to the ratio n / u , where n is the number of the constituent atoms in the primary-ion beam and u is the velocity of the incident ions.In the present Communication, we report the influence of the Cs+ energy in the range 2-35 keV on the relative intensities of the protonated molecules and the extent of fragmentation in the fast-ion mass spectra of some 0-lactam antibiotics.

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“…In some of these newer methods of ionization, the sample is ionized directly from the solid phase, thereby circumventing a major problem with EIMS, the need to volatilize the sample without causing extensive degradation or the necessity for chemical derivatization. 5 At the time of the Borders review the two most promising new ionization methods for non-volatile NPs were eld desorption (FD) and fast atom bombardment (FAB).…”

Section: "Soft" Ionization For the Massesmentioning

confidence: 99%

“…Owing to its relatively high molecular weight (900-1100 amu), narrowing down the number of possible molecular formulaseven with HRFT-MS datawas challenging. By sequentially fragmenting molecular and daughter ions over the course of ve MS/MS cycles (MS 5 ) and calculating accurate mass values for each neutral loss, an unambiguous molecular formula was reconstructed by summing the individual compositions, as shown in Fig. 4.…”

Section: Ms For Structure Determinationmentioning

confidence: 99%

NP/MS since 1970: from the basement to the bench top

Carter

2014

Nat. Prod. Rep.

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This Highlight explores the evolution of applications of mass spectrometric technologies in the context of natural products research since the 1970's. The central themes are the analysis of mixtures, dereplication (identification) and structure determination. The ascension of HPLC as the method of choice for the analysis of pharmaceuticals was a driving force for the development of interfaces for coupling of HPLC and MS. An example of sequential analysis of fragment ions or MS/MS or MS(n) methods to provide detailed structural information on muraymycins, a family of uridyl-peptide antibiotics, is presented.

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Recent applications of mass spectrometry to antibiotic research

Cited by 18 publications

References 164 publications

Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Investigation of some β‐lactam antibiotics by means of 2–35 keV cesium ion bombardment

NP/MS since 1970: from the basement to the bench top

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