Recent progressive use of atomic force microscopy in biomedical applications

Maver, Uroš; Velnar, Tomaž; Gaberšček, Miran; Planinšek, Odon; Finšgar, Matjaž

doi:10.1016/j.trac.2016.03.014

Cited by 118 publications

(62 citation statements)

References 197 publications

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“…Over the last decade, AFM has evolved from a merely imaging technique into a powerful tool of investigation in the life science (see, e.g. the two recent reviews; Maver et al, 2016;Alsteens et al, 2017). In particular, AFM imaging and nanomechanical studies have found a niche in the biomedical research of collagen-containing tissues, because the nano-to microscale level of probing with AFM suits perfectly the hierarchy of collagen structures in the ECM.…”

Section: Discussionmentioning

confidence: 99%

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Свистушкин

Kotova

Shekhter

et al. 2019

Journal of Microscopy

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Summary Regenerative medicine opens new opportunities in the repair of cicatricial lesions of the vocal folds. Here, we present a thorough morphological study, with the focus on the collagen structures in the mucosa of the vocal folds, dedicated to the effects of stem cells on the vocal folds repair after cicatricial lesions. We used a conventional experimental model of a mature scar of the rabbit vocal folds, which was surgically excised with a simultaneous implantation of autologous bone marrow‐derived mesenchymal stem cells (MSC) into the defect. The restoration of the vocal folds was studied 3 months postimplantation of stem cells and 6 months after the first surgery. The collagen structure assessment included histology, immunohistochemistry and atomic force microscopy (AFM) studies. According to the data of optical microscopy and AFM, as well as to immunohistochemical analysis, MSC implantation into the vocal fold defect leads not only to the general reduction of scarring, normal ratio of collagens type I and type III, but also to a more complete restoration of architecture and ultrastructure of collagen fibres in the mucosa, as compared to the control. The collagen structures in the scar tissue in the vocal folds with implanted MSC are more similar to those in the normal mucosa of the vocal folds than to those of the untreated scars. AFM has proven to be an instrumental technique in the assessment of the ultrastructure restoration in such studies. Lay Description Regenerative medicine opens new opportunities in the repair of the vocal fold scars. Because collagen is a main component in the vocal fold mucosa responsible for the scar formation and repair, we focus on the collagen structures in the mucosa of the vocal folds, using a thorough morphological study based on histology and atomic force microscopy (AFM). Atomic force microscopy is a scanning microscopic technique which allows revealing the internal structure of a tissue with a resolution up to nanometres. We used a conventional experimental model of a mature scar of the rabbit vocal folds, surgically excised and treated with a mesenchymal stem cells transplant. Our morphological study, primarily AFM, explicitly shows that the collagen structures in the scarred vocal folds almost completely restore after the stem cell treatment. Thus, the modern microscopic methods, and especially AFM are instrumental tools for monitoring the repair of the vocal folds scars.

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Section: Discussionmentioning

confidence: 99%

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Свистушкин

Kotova

Shekhter

et al. 2019

Journal of Microscopy

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“…e properties of the sample affected by interactions with the AFM tip include friction, adhesion, and high elasticity. Using phase mode, images show differences between the local regions on the sample, and this mood is also useful for testing the consistency of coatings and for displaying cracks and other degradation features [10].…”

Section: Introductionmentioning

confidence: 99%

Scanning Electron Microscopy and Atomic Force Microscopy: Topographic and Dynamical Surface Studies of Blends, Composites, and Hybrid Functional Materials for Sustainable Future

Rydz

Šišková

Eckstein

2019

Advances in Materials Science and Engineering

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Microscopic techniques are often used in material science, enabling the assessment of the morphology, composition, physical properties, and dynamic behaviour of materials. The review focuses on the topographic and dynamical surface studies of (bio)degradable polymers, in particular aliphatic polyesters, the most promising ones. The (bio)degradation process promotes physical and chemical changes in material properties that can be characterised by microscopic techniques. These changes occurring both under controlled conditions as well as in the processing stage or during use indicate morphological and structural transformations resulting from the deterioration of the material and have a significant impact on the characteristic of materials used in many applications, for example, for use as packaging.

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“…Представляя собой один из самых мощных и современных методов исследования рельефа поверхностей различных материалов с разрешением вплоть до атомарного, атомно-силовая микроскопия (АСМ) [1] является востребованной не только в физике, химии и биологии, но и в различных междисциплинарных областях, таких как материаловедение и биофизика [2,3]. К сожалению, традиционно используемые в АСМ кантилеверы из кремния и нитрида кремния, представляющие собой балку с вытравленным острым наконечником (иглой), имеют ряд ограничений [4]: в частности, они слабо пригодны для исследования материалов со сложной биологической структурой (например, белковых макромолекул) или значительной пористостью.…”

Section: Introductionunclassified

Тепловые Флуктуации И Резонансные Свойства Сканирующих Зондов На Основе Углеродных Нанотрубок

Чалин¹,

Avramenko²

2019

Физика Твердого Тела

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The design of cantilevers and microelectromechanical systems based on carbon nanotubes necessitates a thorough study of their thermal fluctuations and resonance properties. This work aims at the simulation of thermal fluctuations and power spectral density for single-walled carbon nanotubes in the context of the continuous model of elastic cylindrical membranes with nonmacroscopic thicknesses. The conditions imposed on the geometry of this probe and providing its stable operation, as well as the high lateral resolution determined by only the radius of the nanotube independently on its thermal vibrations, are found, as well. The most intensive resonance modes, making the greatest contribution to the thermal fluctuations of carbon nanotubes, are established, and their frequencies are found analytically.

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Recent progressive use of atomic force microscopy in biomedical applications

Cited by 118 publications

References 197 publications

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Collagen fibrillar structures in vocal fold scarring and repair using stem cell therapy: a detailed histological, immunohistochemical and atomic force microscopy study

Scanning Electron Microscopy and Atomic Force Microscopy: Topographic and Dynamical Surface Studies of Blends, Composites, and Hybrid Functional Materials for Sustainable Future

Тепловые Флуктуации И Резонансные Свойства Сканирующих Зондов На Основе Углеродных Нанотрубок

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