Recent update on the prevalence ofVibriospecies among cultured grouper in Peninsular Malaysia

Abstract

Vibrio infections are common among marine fish and lead to serious problems in the aquaculture sector. This study reports a recent occurrence of Vibrio species (spp.) isolated from cultured groupers in Peninsular Malaysia using the gyrB and pyrH genes. A total of 147 Vibrio strains were successfully isolated from 77 (64%) groupers using culture method and subjected to gyrB and pyrH sequencing for species identification and confirmation. Results showed that 89% of Vibrio strains were identified and clustered to six groups of Vibrio spp., while 11% were not clustered to any Vibrio spp. using the gyrB sequences. Meanwhile, by analysis of the pyrH sequences all the 147 Vibrio strains (100%) were successfully identified and clustered into 11 groups of Vibrio spp., including the gyrB non‐identified strains. The pyrH gene provides a better resolution for identification of Vibrio spp. compared with the gyrB gene. Thus, the pyrH gene was more suitable for a rapid determination of Vibrio spp. distribution in Peninsular Malaysia. Using the pyrH gene, our study found higher prevalence of Vibrio vulnificus (33%), V. alginolyticus (24%) and V. parahaemolyticus (22%), followed by V. rotiferianus (5%), V. harveyi (3%), V. tubiashii (2%), V. campbellii (2%), V. ponticus (1%), V. diabolicus (1%), V. owensii (1%) and others Vibrio sp. (7%). Thus, the results of this study revealed that the occurrence of pathogenic vibrios among grouper fish is still high in Malaysian aquaculture. In addition, the pyrH gene was proved as a suitable marker for rapid identification of Vibrio species compared with the gyrB gene.

Distribution of Vibrionaceae in farmed Asian sea bass, Lates calcarifer in Thailand and their high prevalence of antimicrobial resistance

“…These results were closed to the recent update of Vibrionaceae isolated from Epinephelus spp. in the Peninsula of Malaysia, which reported V. harveyi , V. vulnificus , V. alginolyticus , V. parahaemolyticus and P. damselae were commonly found around the peninsula of Malaysia (Abdullah et al, 2017; Amalina et al, 2019).…”

Current status and advances of fish vaccines in Malaysia

“…Interestingly, other cultured fish species produced in the exact location, such as Asian seabass, snapper, and golden pompano, were not harmed. More recently, according to Amalina et al [95], the distribution of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia was predominated by V. vulnificus, V. alginolyticus, and V. parahaemolyticus with 33%, 24%, and 22%, respectively.…”

“…Thus, vibriosis has remained a severe problem in Malaysian cultured marine fish since the 90s, resulting in significant economic losses. Total losses among Malaysia's three main grouper, snapper, and seabass culture species were USD 7.4 million in 1990 [95].…”

Ketahanan Ikan Kerapu Hibrida Cantang (Epinephelus fuscoguttatus><Epinephelus lanceotatus) Terhadap Infeksi Virus dan Bakteri

“…Vibrio sp. menimbulkan borok pada sirip (sirip geripis) (Ebi et al, 2018); Amalina et al, 2019). Vibrio sp juga dapat menimbulkan luka atau borok pada ikan (Desrina et al, 2006).…”