Reclamation of beneficial bioactivities of herbal antioxidant condensed tannin extracted from Euonymus laxiflorus

Nguyen, Van Bon; Ton, That Quang; Nguyen, Dai Nam; Nguyen, Thanh Thi; Ngu, Truong Nhan; Nguyen, Thi Hanh; Doan, Chien Thang; Tran, Thi Ngoc; Nguyen, Minh; Ho, Nhat Duoc; Nguyen, Anh Dzung; Kuo, Yao‐Haur; Wang, San-Lang

doi:10.1007/s11164-020-04251-3

Cited by 7 publications

(15 citation statements)

References 36 publications

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“…The anti-nitric oxide effect of PG was also examined (anti-NO, an indicator of pro-inflammatory properties, which is related to some disorders such as rheumatoid arthritis, chronic hepatitis, and pulmonary fibrosis) [ 30 , 49 , 50 , 51 ]. In this report, LPS-stimulated-RAW264.7 cells were used to assess the anti-NO activity of PG, and homogentisic acid was used as the standard for the anti-NO assay.…”

Section: Resultsmentioning

confidence: 99%

Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin

et al. 2021

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Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications. The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG. Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains. Further, PG scale-up production was investigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fermentation using 5 L of a novel-designed culture broth that included 1.60% C/N sources (a de-SSP/casein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6–7. Fermentation was conducted in the dark at 27.5 °C for 8.0 h. This study was the first to report on the utilization of shrimp wastes for cost-effective, large-scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time. The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield. The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra. The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxidant activities, and novel anti-NO effects.

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Section: Resultsmentioning

confidence: 99%

Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin

et al. 2021

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“…The anti-NO effect has been recognized as an indicator of pro-inflammatory properties, which are relevant to various disorders, including chronic hepatitis, rheumatoid arthritis, and pulmonary fibrosis [ 32 , 33 ]. To assess the anti-NO activity of PG, LPS-stimulated-RAW264.7 cells were tested, and a commercial anti-NO compound, homogentisic acid (HGA), was also used for comparison.…”

Section: Resultsmentioning

confidence: 99%

“…Of these, the anti-NO assay was performed following the protocol presented in our previous work, and Homogentisic acid was used as a reference compound for comparison. The antioxidant activity was evaluated using the DPPH and ABTS radical scavenging assays, as presented in prior studies [ 33 , 36 ], respectively, and α-tocopherol was the commercial antioxidant compound included for comparison.…”

Section: Methodsmentioning

confidence: 99%

Utilization of Cassava Wastewater for Low-Cost Production of Prodigiosin via Serratia marcescens TNU01 Fermentation and Its Novel Potent α-Glucosidase Inhibitory Effect

et al. 2021

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The purpose of this study was to reuse cassava wastewater (CW) for scaled-up production, via the fermentation of prodigiosin (PG), and to conduct an evaluation of its bioactivities. PG was produced at the yield of high 6150 mg/L in a 14 L-bioreactor system, when the designed novel medium (7 L), containing CW and supplemented with 0.25% casein, 0.05% MgSO4, and 0.1% K2HPO4, was fermented with Serratia marcescens TNU01 at 28 °C in 8 h. The PG produced and purified in this study was assayed for some medical effects and showed moderate antioxidant, high anti-NO (anti-nitric oxide), and potential α-glucosidase inhibitory activities. Notably, PG was first reported as a novel effective α-glucosidase inhibitor with a low IC50 value of 0.0183 µg/mL. The commercial anti-diabetic drug acarbose was tested for comparison and had a lesser effect with a high IC50 value of 328.4 µg/mL, respectively. In a docking study, the cation form of PG (cation-PG) was found to bind to the enzyme α-glucosidase by interacting with two prominent amino acids, ASP568 and PHE601, at the binding site on the target enzyme, creating six linkages and showing a better binding energy score (−14.6 kcal/mol) than acarbose (−10.5 kcal/mol). The results of this work suggest that cassava wastewater can serve as a low-cost raw material for the effective production of PG, a potential antidiabetic drug candidate.

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“…Some biological effects such as antioxidant capacity, anti-nitric oxide activity, and acetylcholinesterase inhibition were tested. Of these, the DPPH radical scavenging activity assay was used to evaluate the antioxidant effect of PG, and this assay was conducted according to the method in the previous study [65]. The anti-nitric oxide and acetylcholinesterase inhibitory activities were assayed following Nguyen et al 2018 [66] and Tan et al 2028 [39], respectively.…”

Section: Biological Activity Assaysmentioning

confidence: 99%

Bioproduction of Prodigiosin from Fishery Processing Waste Shrimp Heads and Evaluation of Its Potential Bioactivities

Nguyen

Wang

Nguyen

et al. 2021

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The aim of this work was to reuse a fish processing waste, shrimp head powder (SHP), for the production of prodigiosin (PG) via microbial technology and to assess its potential bioactivities. PG was produced in a 12 L-bioreactor system, and the highest PG productivity of 6310 mg L−1 was achieved when Serratia marcescens CC17 was used for fermentation in a novel designed medium (6.75 L) containing 1.5% C/N source (SHP/casein = 9/1), 0.02% K2SO4, ans 0.025% Ca3(PO4)2, with initial pH 7.0, and fermentation was performed at 28 °C for 8 h. The purified PG showed moderate antioxidants, efficient anti-NO (anti-nitric oxide), and acetylcholinesterase (AChE) inhibitory activities. In a docking study, PG showed better binding energy scores (−12.3 kcal/mol) and more interactions (6 linkages) with several prominent amino acids in the biding sites on AChE that were superior to those of Berberine chloride (−10.8 kcal/mol and one linkage). Notably, this is the first investigation using shrimp heads for the mass bioproduction of PG with high productivity, and Ca3(PO4)2 salt was also newly found to significantly enhance PG production by S. marcescens. This study also provided available data on the anti-NO and anti-AChE effects of PG, especially from the docking simulation PG towards AChE that was described for the first time in this study. The above results suggest that SHP is a good material for the cost-effective bioproduction of PG, which is a potential candidate for anti-NO and anti-Alzheimer drugs.

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Reclamation of beneficial bioactivities of herbal antioxidant condensed tannin extracted from Euonymus laxiflorus

Cited by 7 publications

References 36 publications

Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin

Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin

Utilization of Cassava Wastewater for Low-Cost Production of Prodigiosin via Serratia marcescens TNU01 Fermentation and Its Novel Potent α-Glucosidase Inhibitory Effect

Bioproduction of Prodigiosin from Fishery Processing Waste Shrimp Heads and Evaluation of Its Potential Bioactivities

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