Reclassification of Eubacterium formicigenerans Holdeman and Moore 1974 as Dorea formicigenerans gen. nov., comb. nov., and description of Dorea longicatena sp. nov., isolated from human faeces.

Hungatella effluvii gen. nov., sp. nov., an obligately anaerobic bacterium isolated from an effluent treatment plant, and reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov.

“…A phylogenetic tree reconstructed with representatives of other closely related genera (Johnsonella ignava ATCC 51276 T as an outgroup) assigned strain UB-B.2 T to Clostridium rRNA cluster XIV (Collins et al 1994), which comprises more than 20 clostridial species with high DNA G+C content and is divided into two subgroups, XIVa and XIVb. Members of cluster XIVa have been reclassified into various genera based on both phylogenetic and phenotypic characterization (Cook et al, 2007;Liu et al, 2008;Taras et al, 2002). The novel genus 'Lachnoclostridium' (Yutin & Galperin, 2013) was proposed based on phylogenetic comparison to allocate all species of cluster XIVa; however, this classification again accommodates heterogeneous species.…”

“…The detailed phenotypic characteristics of strain UB-B.2 T are given in Tables 1 and 2. Differences between strain UB-B.2 T and C. hathewayi DSM 13479 T were observed for tests including a-arabinosidase, aesculin hydrolysis and utilization of (Holdeman & Moore, 1974;Ezaki, 2009); 8, Roseburia cecicola GM T (Stanton & Savage, 1983); 9, Johnsonella ignava ATCC 51276 T (Moore & Moore, 1994); 10, Acetitomaculum ruminis 139B T (Greening & Leedle, 1989;Rainey et al, 2009); 11, Dorea formicigenerans ATCC 27755 T (Taras et al, 2002). ND, No data available.…”

“…Members of cluster XIVa (also known as the 'Clostridium coccoides-Eubacterium rectale group') have been classified into new genera such as Blautia (Liu et al, 2008), Dorea (Taras et al, 2002), Johnsonella, Catonella, Hallella (Moore & Moore, 1994) and Ruminococcus (Sijpesteijn, 1948) based on their phenotypic characteristics and phylogenetic analysis of the 16S rRNA gene sequence. Although some species of cluster XIVa have been reclassified into new genera, there are still many species that could not be included in any of the proposed genera, suggesting that they may represent novel genera.…”

Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses

“…Some genera of the Clostridium subphylum have been inadequately described (Collins et al, 1994;Kageyama & Benno, 2000a, b; and, because of their broad definitions, these genera have acted as a depository for a large number of phylogenetically diverse species. For example, some species originally assigned to the genus Eubacterium in the Clostridium subphylum have subsequently been transferred to other genera or to new genera (Kageyama et al, 1999a, b;Ludwig et al, 1992;Nakazawa et al, 2000;Taras et al, 2002;Wade et al, 1999;. The clusters of the Clostridium subphylum provide a useful starting point for investigations of phylogenetic relationships among the Gram-positive, nonspore-forming, rod-shaped anaerobic bacteria of the mammalian intestinal tract.…”

Clostridium glycyrrhizinilyticum Sp. Nov., a Glycyrrhizin‐Hydrolysing Bacterium Isolated from Human Faeces

“…The heterogeneity of the genus Eubacterium is due to assignment based on phenotypical characteristics before application of phylogenetic analysis to classification. Therefore, there is a growing consensus that the genus Eubacterium sensu stricto should be restricted to the type species, Eubacterium limosum, and its close phylogenetic relatives (10,20,23). Recently unknown Eubacterium-like non-spore-forming organisms VPI 12708 and five strains were identified as Clostridium scindens by phylogenetic and DNA-DNA homology analysis (12).…”