Recombinant feline parvovirus infection of immunized tigers in central China

Wang, Xingang; Li, Tongyi; Liu, Hongying; Du, Juan; Zhou, Feng; Dong, Yanming; He, Xiaojing; Li, Yongtao

doi:10.1038/emi.2017.25

Cited by 17 publications

(19 citation statements)

References 13 publications

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“…Moreover, the NS1 gene molecular features, as a useful tool in outbreak tracing, were only recently reconsidered [31,32]. Despite the most recent sequence analyses on CPV also including this genomic ORF, there are still limited studies on the FPLV NS1 sequence [30,33,34,35].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Evolutionary Analyses of Carnivore Protoparvovirus 1 NS1 Gene

Mira

Canuti

Purpari

et al. 2019

Viruses

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Carnivore protoparvovirus 1 is the etiological agent of a severe disease of terrestrial carnivores. This unique specie encompasses canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPLV). Studies widely analyzed the main capsid protein (VP2), but limited information is available on the nonstructural genes (NS1/NS2). This paper analyzed the NS1 gene sequence of FPLV and CPV strains collected in Italy in 2009–2017, along with worldwide related sequences. Differently from VP2, only one NS1 amino-acid residue (248) clearly and constantly distinguished FPLV from CPV-2, while five possible convergent amino-acid changes were observed that may affect the functional domains of the NS1. Some synonymous mutation in NS1 were non-synonymous in NS2 and vice versa. No evidence for recombination between the two lineages was found, and the predominance of negative selection pressure on NS1 proteins was observed, with low and no overlap between the two lineages in negatively and positively selected codons, respectively. More sites were under selection in the CPV-2 lineage. NS1 phylogenetic analysis showed divergent evolution between FPLV and CPV, and strains were clustered mostly by country and year of detection. We highlight the importance of obtaining the NS1/NS2 coding sequence in molecular epidemiology investigations.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Evolutionary Analyses of Carnivore Protoparvovirus 1 NS1 Gene

Mira

Canuti

Purpari

et al. 2019

Viruses

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“…Particularly, FPLV lineages infecting domestic cats and wild cats are nearly identical. It is interesting to mention that certain FPLV strain which are less pathogenic in domestic cats can be lethal to big felids such as tigers and lions [32,39,40].…”

Section: Discussionmentioning

confidence: 99%

“…The diversity of carnivore protoparvoviruses has been described in many studies and seems to be represented by a great variety of strains and recombinant forms [27,[30][31][32]. Conversely, there are few studies describing the heterogeneity of FPLV strains and its relevance to the maintenance of these viruses in distinct hosts [13].…”

Section: Introductionmentioning

confidence: 99%

Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis

et al. 2020

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Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was identified by polymerase chain reaction. Samples from these animals were used to isolate FPLV strains in CRFK cells for genome sequencing. Phylogenetic analysis was performed to compare our isolates with available sequences of FPLV, mink parvovirus (MEV) and canine parvovirus (CPV). The isolated strains were closely related to strains of FPLV/MEV isolated in the 1960s. Our analysis also revealed that the evolutionary history of FPLV and MEV is characterized by local adaptations in the Vp2 gene. Thus, it is likely that new FPLV strains are emerging to evade the anti-FPLV immune response. OPEN ACCESS Citation: Leal É, Liang R, Liu Q, Villanova F, Shi L, Liang L, et al. (2020) Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis. PLoS ONE 15(1): e0227705.

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“…Consequently, FPV has a high risk of transmission, posing a great threat to rare wild animals. In addition, FPV is prevalent in the Chinese Siberian tiger (Panthera tigris altaica) population [7,8]. On June 6th 2016, an outbreak of fatal FPV infection among captive Siberian tigers in Zhengzhou Zoo in central China was reported [7].…”

mentioning

confidence: 99%

“…In addition, FPV is prevalent in the Chinese Siberian tiger (Panthera tigris altaica) population [7,8]. On June 6th 2016, an outbreak of fatal FPV infection among captive Siberian tigers in Zhengzhou Zoo in central China was reported [7]. An FPV strain was isolated and identified from a captive Siberian Viruses 2020, 12, 315 2 of 8 tiger in a wildlife park in the Jilin Province, China [8].…”

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confidence: 99%

Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Jiao

Zhang

Liu

et al. 2020

Viruses

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Feline panleukopenia, caused by feline parvovirus (FPV), is a highly infectious disease characterized by leucopenia and hemorrhagic gastroenteritis that severely affects the health of large wild Felidae. In this study, tiger FPV virus-like particles (VLPs) were developed using the baculovirus expression system. The VP2 gene from an infected Siberian tiger (Panthera tigris altaica) was used as the target gene. The key amino acids of this gene were the same as those of FPV, whereas the 101st amino acid was the same as that of canine parvovirus. Indirect immunofluorescence assay (IFA) results demonstrated that the VP2 protein was successfully expressed. SDS-PAGE and Western blotting (WB) results showed that the target protein band was present at approximately 65 kDa. Electron micrograph analyses indicated that the tiger FPV VLPs were successfully assembled and were morphologically similar to natural parvovirus particles. The hemagglutination (HA) titer of the tiger FPV VLPs was as high as 1:218. The necropsy and tissue sections at the cat injection site suggested that the tiger FPV VLPs vaccine was safe. Antibody production was induced in cats after subcutaneous immunization, with a >1:210 hemagglutination inhibition (HI) titer that persisted for at least 12 months. These results demonstrate that tiger FPV VLPs might provide a vaccine to prevent FPV-associated disease in the tiger.

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Recombinant feline parvovirus infection of immunized tigers in central China

Cited by 17 publications

References 13 publications

Molecular Characterization and Evolutionary Analyses of Carnivore Protoparvovirus 1 NS1 Gene

Molecular Characterization and Evolutionary Analyses of Carnivore Protoparvovirus 1 NS1 Gene

Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis

Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

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