“…1,2 In this context, digital microfluidic systems have allowed the use of mature fabrication processes through photolithography techniques 3 and the elimination of the need for external modules or complicated geometries such as pumps or valves. 4,5 Amongst several actuation mechanisms for digital microfluidics, such as electrowetting-on-dielectrics (EWOD), 6 dielectrophoresis (DEP), 7 opto-electrowetting, 8 thermocapillary, 9 magnetic actuation, 10 microfluidic devices based on surface acoustic waves (SAWs)/Lamb waves have proven advantageous as they are able to provide a host of useful effects, including acoustic streaming in droplets, efficient mixing, transportation and jetting, and acceleration of hybridization reactions of biochemical assays. 11,12 More generally, acoustic wave devices are uniquely attractive to generate fully integrated LOC systems, as they can also enable biosensing in addition to liquid actuation, wirelessly, [13][14][15] thus significantly reducing complexity of the system and operation/manufacturing costs.…”