Reconstitution of Experimental Neurogenic Bladder Dysfunction Using Skeletal Muscle-Derived Multipotent Stem Cells

Nitta, Masahiro; Tamaki, Toru; Tono, Kayoko; Okada, Yoshinori; Masuda, Maki; Akatsuka, Akira; Hoshi, Akio; Usui, Yukio; Terachi, Toshiro

doi:10.1097/tp.0b013e3181d45a7f

Cited by 42 publications

(27 citation statements)

References 25 publications

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“…Similarly, although on a much smaller scale, Kinebuchi et al [48] reported that transplanted BMSCs differentiated into striated muscle cells and peripheral nerve cells. In regard to the impressive findings by Hoshi et al [53], it should be noted that in a more recent study [67] the same group of researchers made similar observations regarding the multilineage differentiation of transplanted SkMSCs in a neurogenic bladder dysfunction rat model. However, as mentioned in the ''Stem cells'' subsection, there was evidence that muscle-derived cells had limited differentiation potential and this had led to the reversal from a muscle cell-based therapy to a muscle fiber implantation approach.…”

Section: Histological Assessmentsupporting

confidence: 50%

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

Lin

Lue

2012

Stem Cells and Development

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Section: Histological Assessmentsupporting

confidence: 50%

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

Lin

Lue

2012

Stem Cells and Development

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“…MDSCs tend to differentiate into muscle cells and muscle tissue in the absence of specific differentiation-induction, and differentiate into bone cells, cartilage cells, smooth muscle cells, endothelial cells, and neurons in vitro when specific interventions are performed (Gates et al 2008;Smaldone et al 2009). Therefore, MDSCs are valuable in treating muscular dystrophy (Ambrosio et al 2009), heart disease (Shibuya et al 2010), stress urinary incontinence (Stangel-Wojcikiewicz et al 2010), neurogenic bladder (Nitta et al 2010), bone disease, and central nervous system disease (Matsumoto et al 2009;Xu et al 2010). Nolazco et al (2008) infused MDSCs into penile corpora cavernosa of old rats and observed that they differentiated into smooth muscle cells, resulting in significant improvement of erectile function.…”

Section: Discussionmentioning

confidence: 99%

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Xue

Dong

et al. 2013

Cytotechnology

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This study treated the isolation and passage of muscle-derived stem cells (MDSCs) from rat penile corpora cavernosa, detection of stem cell marker expression, observation of their self-renewal and continuous proliferation, and demonstration of their potential to differentiate into smooth muscle cells in co-culture. Muscle-derived stem cells from the rat penile corpora cavernosa were isolated and purified. The expression of stem cell markers Sca-1 and desmin was detected in PP6 cells, thus confirming that the main components of PP6 cells are MDSCs. The expression of Sca-1 and desmin occurred both in PP6 cells and cells at passages 3, 6, and 8, and there was no significant decrease in the expression level with increasing passage number. The growth curves indicated that the cell doubling time was approximately 48 h. The cells entered the stationary phase after approximately 7 days of culture. The proliferative activity of the cells at passage 8 remained unchanged. After 2 days of co-culture with smooth muscle cells, the DAPI-labeled MDSCs tended to exhibit smooth muscle cell morphology and expression of a-SMA was detected. MDSCs exist in the rat penile corpora cavernosa and possess the potential to differentiate into smooth muscle cells. This discovery serves as the basis in view of the potential use of endogenous stem cells for the treatment of erectile dysfunction (ED).

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“…Other studies have shown the power of MDSCs to restore muscular contraction of the muscular sphincter and to recover the damaged pelvic nerves [21,22]. It has also been suggested that the formation of myotubes may activate intrinsic nerve regeneration and formation of the neuromuscular junction [23,27,28]. Furthermore, studies on the pig model showed an increase in urethral pressure profile and muscular myofibrils after injection of MDSCs into the striated urinary sphincter [24].…”

Section: Animal Studiesmentioning

confidence: 99%

Stem Cell Therapy for Male Urinary Incontinence

et al. 2012

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Introduction: Among the medical and surgical options which have been proposed in the last years for the management of male stress urinary incontinence (SUI), stem cell therapy represents a new frontier treatment. The aim of this paper is to update the current status of stem cell therapy in animal and human studies for the management of iatrogenic male SUI. Material and Methods: A PubMed review of the literature on stem cell therapy for the treatment of male SUI was performed. Results: Regarding animal studies, bone marrow-, muscle- and adipose-derived stem cells have been widely studied, showing regeneration of the urethral sphincter and recovery of the damaged pelvic nerves. With regard to human studies, only four papers are available in the literature using muscle- and adipose-derived stem cells which reported a significant improvement in sphincteric function and incontinence with no severe side effects. Conclusions: In spite of these promising results, further studies are needed with longer follow-ups and larger numbers of patients in order to clarify the potential role of stem cell therapy for the treatment of male SUI.

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Reconstitution of Experimental Neurogenic Bladder Dysfunction Using Skeletal Muscle-Derived Multipotent Stem Cells

Cited by 42 publications

References 25 publications

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Stem Cell Therapy for Male Urinary Incontinence

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