Reconstructed human skin: From photodamage to sunscreen photoprotection and anti-aging molecules

Vioux-Chagnoleau, Corinne; Lejeune, François; Sok, Juliette; Pierrard, Cécile; Marionnet, Claire; Bernerd, Françoise

doi:10.1016/j.descs.2006.08.001

Cited by 20 publications

(26 citation statements)

References 60 publications

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“…General morphology as well as sunburn cell formation was monitored using classical histology. [6] Immunostainings were performed using monoclonal antibodies directed against thymidine dimers [14] to detect DNA lesions and against vimentin (Monosan) to label dermal fibroblasts. The amount of released matrix metalloproteinase-1 (MMP-1) was assessed using enzyme-linked immunosorbent assay (ELISA) technique on culture medium.…”

Section: Reconstructed Skinmentioning

confidence: 99%

“…Then, the culture was raised upwards to air-liquid interface for an additional 7 days to allow the keratinocytes to differentiate completely and build a horny layer. [6] Reconstructed pigmented epidermis Epidermis was reconstructed according to the technique described by Régnier et al [7][8][9][10][11][12] Deepidermized dermis (DED) was placed, with basement membrane side up, in a Petri dish. Normal human melanocytes and keratinocytes were co-seeded onto the DED at 10:1 ratio (total 5 × 10 5 cells) into a stainless steel ring.…”

Section: Reconstructed Skinmentioning

confidence: 99%

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Solar ultraviolet radiation induces biological alterations in human skin in vitro: Relevance of a well-balanced UVA/UVB protection

Bernerd¹,

Marionnet²,

Duval³

2012

Indian J Dermatol Venereol Leprol

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Cutaneous damages such as sunburn, pigmentation, and photoaging are known to be induced by acute as well as repetitive sun exposure. Not only for basic research, but also for the design of the most efficient photoprotection, it is crucial to understand and identify the early biological events occurring after ultraviolet (UV) exposure. Reconstructed human skin models provide excellent and reliable in vitro tools to study the UV-induced alterations of the different skin cell types, keratinocytes, fibroblasts, and melanocytes in a dose- and time-dependent manner. Using different in vitro human skin models, the effects of UV light (UVB and UVA) were investigated. UVB-induced damages are essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damages are mostly located within the dermal compartment. Pigmentation can also be obtained after solar simulated radiation exposure of pigmented reconstructed skin model. Those models are also highly adequate to assess the potential of sunscreens to protect the skin from UV-associated damage, sunburn reaction, photoaging, and pigmentation. The results showed that an effective photoprotection is provided by broad-spectrum sunscreens with a potent absorption in both UVB and UVA ranges.

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Section: Reconstructed Skinmentioning

confidence: 99%

Section: Reconstructed Skinmentioning

confidence: 99%

Solar ultraviolet radiation induces biological alterations in human skin in vitro: Relevance of a well-balanced UVA/UVB protection

Bernerd¹,

Marionnet²,

Duval³

2012

Indian J Dermatol Venereol Leprol

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“…Some plants or their extracts possess several other constituents that may have the capability of inhibiting various enzyme such as HYAL, elastase, and matrix metalloproteinases (MMP) enzyme that play a key role in skin aging. [15] Some of them may possess the free radical scavenging or antioxidant property to fight against the signs of aging. Following sections explains the detailed mechanism involved in skin aging.…”

Section: Molecular Mechanisms and Plant Inhibitorsmentioning

confidence: 99%

Untitled

Garg

2017

IJGP

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Skin ageing is a complex multifactorial process occurring in all living beings. It generally comprises two independent and distinct processes, i.e., intrinsic, age-dependent or chronological aging and premature aging, or photoaging which is the result of undue exposure of the skin to ultraviolet (UV) radiations. Photoaging can be prevented or the effects of photoaging can be reduced by the use of certain anti-aging and anti-wrinkle agents. The extracellular matrix (ECM) which forms the outermost part of skin comprises fibroblasts and proteins such as collagen and elastin. Degradation of ECM is directly linked to skin aging and is responsible for the increase in activity of certain enzymes such as collagenase, elastase, and hyaluronidase (HYAL) that are involved in skin aging. With aging there is decrease in the levels of elastin, collagen, and hyaluronic acid which leads to loss of strength and flexibility of skin and appear as wrinkles on the skin. Further, the increase in the activity of collagenase, elastase, and HYAL is triggered by the high levels of reactive oxygen species produced when the skin is exposed to excessive UV radiations leading to skin aging. Today, in the age of modern science and advanced technology, although many techniques such as laser rejuvenation, plastic surgery, and lots of synthetic products such as sunscreen lotions, creams are available yet, there is a place for natural, herbal anti-aging cosmetics. Various botanical extracts have the power to reduce the appearance of skin aging and enhance the beauty of the skin. Medicinal plants have several phytoconstituents such as polyphenols, alkaloids, tannins, saponins, carotenoids, and terpenoids which possess antioxidant properties and can be used in treating the signs of aging. Some plants contain phenolic compounds which have free-radical scavenging property and can suppress aging. Some plants or their extracts possess several other constituents that may have the capability of inhibiting various enzyme such as HYAL, elastase, and matrix metalloproteinases enzyme that play a major role in skin aging. Some of them may possess the free radical scavenging or antioxidant property to fight against the signs of aging. This review focuses on the compilation of medicinal plants and natural compounds as anti-aging and anti-wrinkling agents along with their chemical constituents based on their possible molecular mechanism of action. The present review will facilitate the scientists working in this area for the development of new anti-aging and anti-wrinkle formulations with better efficiency and safety.

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“…Os raios UVB, os mais energéticos comprimentos de onda que atingem a Terra, podem induzir lesões diretamente no DNA. Já os raios UVA são menos energéticos que os UVB, mas têm maior poder de penetração na pele (VIOUX-CHAGNOLEAU et al, 2006). Assim, as radiações UVB e UVA diferem quanto aos seus sítios de ação na geração de lesões.…”

Section: A Radiação Ultravioleta E Os Danos Causados à Peleunclassified

Avaliação in vitro e in vivo do potencial fotoquimiopreventivo do extrato de Lychnophora salicifolia Mart. e do ácido clorogênico livres e incorporados em lipossomas

Forte¹

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A pele possui um grande número de mecanismos de defesa antioxidante. No entanto, a exposição prolongada à radiação ultravioleta (RUV), é capaz de aumentar a concentração de espécies reativas de oxigênio (EROs) provocando um desequilíbrio antioxidante/oxidante. A geração de EROs pode levar ao fotoenvelhecimento e doenças, como o câncer. A administração tópica de antioxidantes é uma maneira eficiente de enriquecer o sistema protetor cutâneo endógeno e, assim, uma estratégia para reduzir os danos oxidativos causados pela RUV à pele. Entretanto, para fornecer a proteção adequada à pele, estas substâncias devem ultrapassar a barreira imposta pelo estrato córneo. O extrato de Lychnophora salicifolia Mart. possui compostos com atividades antioxidante, antiinflamatória e analgésica, como o ácido clorogênico e a vicenina-2, sendo, portanto, um possível candidato à agente protetor dos danos induzidos pela radiação na pele. Assim, o objetivo do presente trabalho foi avaliar o potencial fotoquimiopreventivo deste extrato e do ácido clorogênico, bem como de formulações contendo o extrato e a substância isolada encapsulados em lipossomas. Para atingir tal objetivo, foram realizados experimentos in vitro e in vivo de atividade antioxidante e eficácia fotoquimiopreventiva para comprovação do potencial antioxidante e anti-inflamatório, estudos de liberação e estudos de penetração cutânea das formulações utilizando pele humana e pele de orelha de porco. Os resultados obtidos demonstraram que o extrato possui atividade antioxidante in vitro e baixa citotoxicidade. A incorporação do extrato e da substância isolada em lipossomas foi bem sucedida, uma vez que estes demonstraram uma boa estabilidade físico-química e aumentaram a penetração dos compostos na pele de camundongos sem pelos. As formulações contendo lipossomas permitiram que os compostos do extrato e o ácido clorogênico isolado atravessassem o estrato córneo chegando até epiderme e derme na pele humana ex vivo. O acúmulo de partículas nos folículos pilosos parece não exercer influencia neste processo. Os extrato foi capaz de sequestrar EROs intracelulares e evitar a depleção do antioxidante endógeno GSH tanto em cultura de células como nos experimentos in vivo. Também foi observada a inibição in vivo da metaloproteinase de matriz-9 (MMP-9), enzima ativada pela RUV e que tem a capacidade de degradar os componentes da matriz extracelular da pele. Além disso, o extrato apresentou atividade anti-inflamatória superior à do ácido clorogênico, verificada pela diminuição dos níveis de citocinas pró-inflamatórias (IL-1β, IL-6 e TNF-α) e da atividade da enzima mieloperoxidase (MPO) in vivo. Portanto, o extrato parece ser um agente fotoquimiopreventivo promissor para uso tópico e, as formulações desenvolvidas, um sistema viável para liberação destas substâncias na pele.Palavras-Chave: Ácido clorogênico, Fotoquimioprevenção, Lipossomas flexíveis, Lychnophora salicifolia, Radiação ultravioleta. The skin has several defense mechanisms to avoid oxidant damages. However, a prolonged exposi...

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Reconstructed human skin: From photodamage to sunscreen photoprotection and anti-aging molecules

Cited by 20 publications

References 60 publications

Solar ultraviolet radiation induces biological alterations in human skin in vitro: Relevance of a well-balanced UVA/UVB protection

Solar ultraviolet radiation induces biological alterations in human skin in vitro: Relevance of a well-balanced UVA/UVB protection

Untitled

Avaliação in vitro e in vivo do potencial fotoquimiopreventivo do extrato de Lychnophora salicifolia Mart. e do ácido clorogênico livres e incorporados em lipossomas

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