Recovery of astaxanthin using colloidal gas aphrons (CGA): A mechanistic study

Dermiki, Maria; Gordon, M. H.; Jauregi, Paula

doi:10.1016/j.seppur.2007.12.023

Cited by 30 publications

(33 citation statements)

References 34 publications

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“…This is in agreement with what was found in the case of the standard suspension of astaxanthin, where ζ = −53 mV. 28 In the case of the cationic surfactant CTAB, the zeta potential of the supernatant was positive. This can be ascribed to the fact that the released astaxanthin from the cells was wetted by the surfactant molecules of CTAB.…”

Section: Results and Discussion Cell Disruption Experimentssupporting

confidence: 90%

“…The volumes of CGA used were based on the results of the standard suspension experiments, so when 10 mL or 30 mL of CGA was used, it was expected to obtain maximum and lower recovery, respectively. 28 It was observed that the highest recovery of astaxanthin from cells (Table 4) was achieved when the cells were dispersed in a solution of NaOH (0.2 mol L −1 ), which is in agreement with the recovery results when pure astaxanthin was dispersed in standard suspensions and recovered with CGA generated from the cationic surfactant CTAB. 28 In our previous study, higher recoveries were achieved when astaxanthin was dispersed in a solution of basic pH compared to the case when astaxanthin was dispersed in water or in an acidic solution.…”

Section: Recovery Of Astaxanthin Using Cga Conventional Integrated Apsupporting

confidence: 82%

“…28 It was observed that the highest recovery of astaxanthin from cells (Table 4) was achieved when the cells were dispersed in a solution of NaOH (0.2 mol L −1 ), which is in agreement with the recovery results when pure astaxanthin was dispersed in standard suspensions and recovered with CGA generated from the cationic surfactant CTAB. 28 In our previous study, higher recoveries were achieved when astaxanthin was dispersed in a solution of basic pH compared to the case when astaxanthin was dispersed in water or in an acidic solution. Furthermore, low recovery of astaxanthin from cells was achieved in the case of cells pretreated in HCl and water (Table 4), which was in agreement with the results of the standard suspension experiments.…”

Section: Recovery Of Astaxanthin Using Cga Conventional Integrated Apsupporting

confidence: 82%

“…Interestingly these results are in agreement with findings from the standard suspension experiments, where the recovery of astaxanthin in the aphron phase after pre-treatment with CTAB did not exceed 40%. 28 However, although the recovery of total carotenoids was significantly higher in the case of cells treated with NaOH solutions, the selectivity in relation to the biomass was low. The biomass partitioned between the two phases in similar proportions to the astaxanthin.…”

Section: Recovery Of Astaxanthin Using Cga Conventional Integrated Apmentioning

confidence: 96%

“…The conditions for optimal recovery involved the use of the cationic surfactant cetyl trimethyl ammonium bromide (CTAB) and the pH and the ionic strength of the solution were adjusted so that the particles of astaxanthin possessed the maximum possible negative surface charge in order to be attracted by the positively charged CGA generated from the cationic surfactant. 28 The optimum conditions obtained from the standard solution experiments will be applied for the recovery of astaxanthin from the cells of Phaffia rhodozyma. However, in this case it will be necessary to disrupt the cells and at the same time recover the product with the highest possible purity.…”

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confidence: 99%

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The use of colloidal gas aphrons as novel downstream processing for the recovery of astaxanthin from cells ofPhaffia rhodozyma

Dermiki

Gordon

Jauregi

2008

J of Chemical Tech & Biotech

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Section: Results and Discussion Cell Disruption Experimentssupporting

confidence: 90%

Section: Recovery Of Astaxanthin Using Cga Conventional Integrated Apsupporting

confidence: 82%

Section: Recovery Of Astaxanthin Using Cga Conventional Integrated Apsupporting

confidence: 82%

Section: Recovery Of Astaxanthin Using Cga Conventional Integrated Apmentioning

confidence: 96%

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confidence: 99%

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The use of colloidal gas aphrons as novel downstream processing for the recovery of astaxanthin from cells ofPhaffia rhodozyma

Dermiki

Gordon

Jauregi

2008

J of Chemical Tech & Biotech

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Extraction of natural red colorants from the fermented broth ofPenicillium purpurogenumusing aqueous two-phase polymer systems

Santos-Ebinuma

Lopes

Teixeira

2015

Biotechnol Progress

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Safety concerns related to the increasing and widespread application of synthetic coloring agents have increased the demand for natural colorants. Fungi have been employed in the production of novel and safer colorants. In order to obtain the colorants from fermented broth, suitable extraction systems must be developed. Aqueous two-phase polymer systems (ATPPS) offer a favorable chemical environment and provide a promising alternative for extracting and solubilizing these molecules. The aim of this study was to investigate the partitioning of red colorants from the fermented broth of Penicillium purpurogenum using an ATPPS composed of poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA). Red colorants partitioned preferentially to the top (PEG-rich phase). In systems composed of PEG 6,000 g/mol/NaPA 8,000 g/mol, optimum colorant partition coefficient (KC ) was obtained in the presence of NaCl 0.1 M (KC = 10.30) while the PEG 10,000 g/mol/NaPA 8,000 g/mol system in the presence of Na2 SO4 0.5 M showed the highest KC (14.78). For both polymers, the mass balance (%MB) and yield in the PEG phase (%ηTOP ) were close to 100 and 79%, respectively. The protein selectivity in all conditions evaluated ranged from 2.0-3.0, which shows a suitable separation of the red colorants and proteins present in the fermented broth. The results suggest that the partitioning of the red colorants is dependent on both the PEG molecular size and salt type. Furthermore, the results obtained support the potential application of ATPPS as the first step of a purification process to recover colorants from fermented broth of microorganisms.

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Separation of astaxanthin from cells of Phaffia rhodozyma using colloidal gas aphrons in a flotation column

Dermiki

Bourquin

Jauregi

2009

Biotechnology Progress

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The aim of this study is to investigate the separation of astaxanthin from the cells of Phaffia rhodozyma using colloidal gas aphrons (CGA), which are surfactant stabilized microbubbles, in a flotation column. It was reported in previous studies that optimum recoveries are achieved at conditions that favor electrostatic interactions. Therefore, in this study, CGA generated from the cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) were applied to suspensions of cells pretreated with NaOH. The different operation modes (batch or continuous) and the effect of volumetric ratio of CGA to feed, initial concentration of feed, operating height, and flow rate of CGA on the separation of astaxanthin were investigated. The volumetric ratio was found to have a significant effect on the separation of astaxanthin for both batch and continuous experiments. Additionally, the effect of homogenization of the cells on the purity of the recovered fractions was investigated, showing that the homogenization resulted in increased purity. Moreover, different concentrations of surfactant were used for the generation of CGA for the recovery of astaxanthin on batch mode; it was found that recoveries up to 98% could be achieved using CGA generated from a CTAB solution 0.8 mM, which is below the CTAB critical micellar concentration (CMC). These results offer important information for the scale-up of the separation of astaxanthin from the cells of P. rhodozyma using CGA.

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Recovery of astaxanthin using colloidal gas aphrons (CGA): A mechanistic study

Cited by 30 publications

References 34 publications

The use of colloidal gas aphrons as novel downstream processing for the recovery of astaxanthin from cells ofPhaffia rhodozyma

The use of colloidal gas aphrons as novel downstream processing for the recovery of astaxanthin from cells ofPhaffia rhodozyma

Extraction of natural red colorants from the fermented broth ofPenicillium purpurogenumusing aqueous two-phase polymer systems

Separation of astaxanthin from cells of Phaffia rhodozyma using colloidal gas aphrons in a flotation column

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