Recovery of <i>Bordetella bronchiseptica</i> sequence type 82 and <i>B. pseudohinzii</i> from urban rats in Terengganu, Malaysia

Abstract

Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder.

Characterisation of Bordetella bronchiseptica isolated from rabbits in Fujian, China

“…Ten antibiotics with the stated concentrations (μg/disc) were included: ampicillin (10), cefixime (5), cefotaxime (30), ceftizoxime (30), gentamycin (10), kanamycin (30), streptomycin (10), ofloxacin (5), ciprofloxacin (5) and norfloxacin (10). The results were interpreted based on the breakpoints for Enterobacteriaceae of the CLSI standards [21,22]. The Staphylococcus aureus ATCC 29213 was included as the quality control.…”

Isolation and characterization of Bordetella pseudohinzii in mice in China

“…Although there is no evidence that B. pseudohinzii could infect humans, B. hinzii which shares high similarity with B. pseudohinzii has been isolated from humans and is associated with mutiple diseases . B. hinzii has been isolated from wild rodents, but the source of transmission remains elusive . The isolation of B. pseudohinzii in wild rodents together with our finding that the bacterica can shed through the feces and transmit to other animals raises public health concerns .…”

“…B. hinzii has been isolated from wild rodents, but the source of transmission remains elusive . The isolation of B. pseudohinzii in wild rodents together with our finding that the bacterica can shed through the feces and transmit to other animals raises public health concerns . Wild rodents represent an important reservoir of multiple human pathogens .…”

“…To date, B. pseudohinzii has been isolated not only from laboratory mice, but also from wild rats . Although there is no evidence that B. pseudohinzii could infect humans, B. hinzii which shares high similarity with B. pseudohinzii has been isolated from humans and is associated with mutiple diseases .…”

Characterization of Bordetella Bronchiseptica Isolated from Rabbits in Fujian, China

“…Ten antibiotics including ampicillin, cefixime, cefotaxime, ceftizoxime, gentamycin, kanamycin, streptomycin, ofloxacin, ciprofloxacin and norfloxacin were used. The results were interpreted based on the breakpoints for Enterobacteriaceae of the CLSI standards[27,28]. The Staphylococcus aureus ATCC 29213 was included as the quality control.…”