Red cell hemolysis during processing and storage

Sawant, Rajesh B; Jathar, SK; Rajadhyaksha, SB; Kadam, PT

doi:10.4103/0973-6247.33446

Cited by 83 publications

(94 citation statements)

References 14 publications

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“…Third, the study design was an ex vivo model consisting only of stored blood components, which have been shown to be more fragile and prone to hemolysis. 13,14 Fourth, while we chose to assess pHb as our measure of hemolysis, the use of a modified index of hemolysis system would provide a more standardized measure of hemolysis that would allow some comparison with previous studies. However, we felt it that this measure didn't accurately account for the baseline degree of hemolysis that occurs over time in the absence of the ECMO circuit.…”

Section: Discussionmentioning

confidence: 99%

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Circuit Oxygenator Contributes to Extracorporeal Membrane Oxygenation–Induced Hemolysis

et al. 2015

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Hemolysis can occur as a consequence of extracorporeal membrane oxygenation (ECMO) and is associated with increased mortality and morbidity. Shear stress generated by flow through the circuit and oxygenator is believed to cause ECMO-induced hemolysis. We hypothesize that either a smaller dimension oxygenator or an in-line hemofilter will increase ECMO-associated hemolysis. Circuits were configured with a Quadrox-D Adult oxygenator (surface area 1.8 m2), Quadrox-iD Pediatric oxygenator (surface area 0.8 m2), or Quadrox-D Adult oxygenator with an in-line hemofilter (N=4) and ran for six hours. Samples were collected hourly from the ECMO circuit and a time-based hemolysis control. Plasma hemoglobin levels were assayed. Circuit-induced hemolysis at each time point was defined as the change in plasma hemoglobin standardized to the time-based hemolysis control. Plasma hemoglobin increased with the use of the smaller dimension pediatric oxygenator as compared to the adult oxygenator when controlling for ECMO run time (p=0.02). Further, there was a greater pressure gradient with the smaller dimension pediatric oxygenator (p<0.05). Plasma hemoglobin did not change with the addition of the in-line hemofilter. The use of a smaller dimension pediatric oxygenator resulted in greater hemolysis and a higher pressure gradient. This may indicate that increased shear forces augment ECMO-induced hemolysis.

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Section: Discussionmentioning

confidence: 99%

“…9-12 Hemolysis may be further exacerbated by the use of stored packed red blood cells (pRBCs) required to prime the pump and for transfusions. 13,14 …”

Section: Introductionmentioning

confidence: 99%

Circuit Oxygenator Contributes to Extracorporeal Membrane Oxygenation–Induced Hemolysis

et al. 2015

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“…A standard hemoglobin concentration curve is generated and used to determine the level of free hemoglobin in serum samples. Serums with a concentration of hemoglobin greater than 2 mg/ml are considered to be hemolyzed [157] and excluded from further testing. Other approaches to measure hemolysis include the Harboe assay [158] and measuring at 414 nm [159].…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Circulating microRNAs: potential biomarkers for common malignancies

Khoury

Tran

2015

Biomark. Med.

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MicroRNAs (miRNAs) belong to a class of small noncoding RNAs (ncRNAs), which regulate gene expression at the post-transcriptional level. They are approximately 22 nucleotide sequences in length and have been predicted to control expression of up to 30-60% of all protein-coding genes in mammals. Considering this wide involvement in gene control, aberrant miRNA expression has a strong association with the presence and progression of a disease, hence generating much anticipation in using miRNAs as biomarkers for the diagnosis and prognosis of human cancers. The majority of these miRNAs are intracellular, but recently they have been discovered in bodily fluids. This review will provide an insight into these circulatory miRNA molecules and discuss their potential as cancer biomarkers.

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“…Two recent studies from India reported on the hemolysis levels in SAGM and AS-1 stored RBC components; however different blood processing procedures had been used for the SAGM packs and AS-1 packs, which made it impossible to distinguish between the effects of processing or the additive solution. 10,11 The most solid evidence of differences between RBCs stored in SAGM and AS-1 additive solutions was reported by Hess and colleagues, 12 who analysed large data sets of routine hemolysis quality control data and showed significantly higher hemolysis in RBCs stored in SAGM compared to AS-1. These results were from unpaired data collected over several years from a single blood center and although the same general processing practices were used, differences could not be excluded.…”

Section: Introductionmentioning

confidence: 99%

In vitro measures of membrane changes reveal differences between red blood cells stored in saline‐adenine‐glucose‐mannitol and AS‐1 additive solutions: a paired study

et al. 2013

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Background Saline-Adenine-Glucose-Mannitol (SAGM) and a variant solution, AS-1 have been used for over 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM-RBCs and AS-1-RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC-endothelial cell (EC) interaction. Study Design and Methods Two whole blood packs were pooled-and-split and RBCs prepared (n=6 pairs). One pack was suspended in SAGM and one in AS-1. Samples were collected during 42 days of refrigerated storage. RBC shape/size, glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine parameters (pH, hemolysis) were also measured. Results Compared to SAGM-RBCs, AS-1-RBCs had lower hemolysis (p<0.04), lower GPA+ MPs (p<0.03) and lower PS+ MPs (p<0.03) from day 14 onwards. AS-1-RBCs had higher (p<0.02) side scatter from day 28 onwards, compared to SAGM-RBCs. SAGM-RBCs were more adherent to ECs at day 28 of storage compared to AS-1 RBCs (p=0.04), but reversed at day 42 (p=0.02). No significant differences in forward scatter or pH were found. Conclusion SAGM-RBCs lose more membrane during storage. SAGM-RBCs had increased adherence to ECs at day 28 of storage, while AS-1-RBCs were more adherent at day 42. The effect of these differences on the function and survival of SAGM-RBCs and AS-1-RBCs following transfusion remains to be determined.

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Red cell hemolysis during processing and storage

Cited by 83 publications

References 14 publications

Circuit Oxygenator Contributes to Extracorporeal Membrane Oxygenation–Induced Hemolysis

Circuit Oxygenator Contributes to Extracorporeal Membrane Oxygenation–Induced Hemolysis

Circulating microRNAs: potential biomarkers for common malignancies

In vitro measures of membrane changes reveal differences between red blood cells stored in saline‐adenine‐glucose‐mannitol and AS‐1 additive solutions: a paired study

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