Red cell lipid peroxidation and antioxidant system in haemodialysed patients: Influence of recombinant human erythropoietin (r-HuEPO) treatment

Abstract

In order to determine the role of correcting anaemia with r-HuEPO on susceptibility of red cell to lipid peroxidation, 15 patients on maintenance haemodialysis treated with r-HuEPO for at least one year (group I), 15 patients on maintenance haemodialysis without r-HuEPO (group II) and 30 persons as a control group (group III) were included in the study. We measured erythrocyte superoxide dismutase (e-SOD), erythrocyte catalase (e-CAT) activities, plasma malonyldialdehyde (p-MDA) and serum vitamin E levels in all patients. The healthy controls (group III) had significantly lower levels of p-MDA in comparison to those measured in haemodialysed patients (group I-II) (p < 0.0001). e-SOD activity in group III was significantly higher than in groups I and II, respectively (p < 0.0001 and p < 0.00001), and e-SOD activity was significantly lower in group II than in group I (p < 0.0001). e-CAT activity in group III was higher than in groups I and II, respectively (p < 0.0001 and p < 0.00001) and it was significantly lower in group II than in group I (p < 0.0001). Vitamin E concentration in group III was lower than in group I and group II, respectively (p < 0.0001 and p < 0.0001). But there is no difference between groups I and II. This study suggests that r-HuEPO therapy improves anaemia by decreasing lipid peroxidation and increasing antioxidant activity.

Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients

“…Indeed, a significant increase in the concentration of plasma MDA was observed in these patients (Table 2). Among patients presenting chronic renal failure, whether undergoing hemodialysis or not, several factors such as the lipid composition of platelet membranes (20), lipoprotein abnormalities (6,22), types of dialysis membranes (23) and accumulation of aluminum can trigger platelet activation. It has been reported in the literature that chronic renal failure patients undergoing prolonged hemodialysis show increased levels of aluminum in their sera (24)(25)(26)(27).…”

“…Neiva et al levels of plasma lipid peroxidation compared to healthy individuals (4) and also develop atherosclerosis (5). Some manifestations, such as accelerated aging and atherosclerosis, may be related to the hyperproduction of free radicals (6,7).…”

Central Nervous System Frontiers for the Use of Erythropoietin

“…Because EPO protects against neuronal death induced by NO-generating agents [42] and inhibits iNOS activity stimulated by cytokines [57][58][59], it has been postulated that EPO may up-regulate the expression of antioxidant factors such as superoxide dismutase, glutathione peroxidase, and catalase in neurons and erythrocytes [42,55]. Other evidence for EPO's antioxidant properties stems from observations that in vitro epoetin alfa protects against erythrocyte membrane damage caused by hydroxyl radicals [53] and from reduced lipid peroxidation and enhanced antioxidant activities in patients with anemia of chronic kidney disease treated with epoetin alfa [54,55].…”

“…To investigate whether Prevention of glutamate toxicity [23,24,42] Reduction of apoptosis [44,52] Anti-inflammatory effects [25,42] Antioxidant effects [42,[53][54][55] Stimulation of angiogenesis [56] epoetin alfa might exert a neuroprotective effect during spinal cord ischemic injury in vivo, ischemia was induced in rabbits by occlusion of the abdominal aorta for 20 min [48]. Immediately after release of the occlusion, animals received either normal saline or 350, 800, or 1000 U/kg of epoetin alfa [48].…”

Polyphenolic Extract ofIchnocarpus FrutescensAttenuates Diabetic Complications in Streptozotocin-Treated Diabetic Rats

“…There may be agents that act to inhibit the generation, propagation, or activity of free radicals, such as preventive antioxidants, which include CAT, SOD, GSH-Px, polyphenol, and chain terminators such as vitamins E and C and glutathione. [42,43] Therefore, the measurement of oxidative stress could be useful to investigate its role in the initiation and development of …”