Reduced graphene oxide-grafted bovine serum albumin/bredigite nanocomposites with high mechanical properties and excellent osteogenic bioactivity for bone tissue engineering

Askari, Esfandyar; Rasouli, Mohammad; Darghiasi, Seyedeh Farnaz; Naghib, Seyed Morteza; Zare, Yasser; Rhee, Kyong-Yop

doi:10.1007/s42242-020-00113-4

Cited by 23 publications

(13 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After incubation of plate for 4 h at 37°C, the previous solution was slowly removed and followed by 500 μL of DMSO solution added to each well. The cell culture plates were returned to the incubator for 1 h. The absorbance was measured at 570 nm using a BioTek plate reader and data was reported as cell viability ( Askari et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and investigation on microstructural, mechanical features of mesoporous hardystonite/reduced graphene oxide nanocomposite for medical applications

Bagherpour

Yaghtin

Naghib

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

“…According to the manufacturing protocol, ALP Kit (Pars Azmun, Iran) was utilized for the experiment. Absorbance of wells was recorded using 405 nm plate reader ( Askari et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Synthesis and investigation on microstructural, mechanical features of mesoporous hardystonite/reduced graphene oxide nanocomposite for medical applications

Bagherpour

Yaghtin

Naghib

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, nanozirconium dioxide powder was blended in PCL, and Young’s modulus and compressive strength increased by ~0.4 and 0.5 times, respectively, as compared with the PCL-only scaffold[ 87 ]. In another case, bredigite was mixed with nanosheets of graphene oxide which was reduced by bovine serum albumin, and the addition of reduced graphene oxide enhanced the mechanical properties of scaffolds[ 88 ].…”

Section: Consideration Of Bioprinting a Bonementioning

confidence: 99%

Up-to-date progress in bioprinting of bone tissue

Wu¹,

Li²,

Su³

et al. 2022

IJB

View full text Add to dashboard Cite

The major apparatuses used for three-dimensional (3D) bioprinting include extrusion-based, droplet-based, and laser-based bioprinting. Numerous studies have been proposed to fabricate bioactive 3D bone tissues using different bioprinting techniques. In addition to the development of bioinks and assessment of their printability for corresponding bioprinting processes, in vitro and in vivo success of the bioprinted constructs, such as their mechanical properties, cell viability, differentiation capability, immune responses, and osseointegration, have been explored. In this review, several major considerations, challenges, and potential strategies for bone bioprinting have been deliberated, including bioprinting apparatus, biomaterials, structure design of vascularized bone constructs, cell source, differentiation factors, mechanical properties and reinforcement, hypoxic environment, and dynamic culture. In addition, up-to-date progress in bone bioprinting is summarized in detail, which uncovers the immense potential of bioprinting in re-establishing the 3D dynamic microenvironment of the native bone. This review aims to assist the researchers to gain insights into the reconstruction of clinically relevant bone tissues with appropriate mechanical properties and precisely regulated biological behaviors.

show abstract

“…However, there are certain situations in which full regeneration of bone tissue is not feasible and needs further stimulation 2 . Biomaterials are a viable alternative to bone transplants in bone tissue engineering 3 , 4 . Synthetic hydroxyl apatite, tri-calcium phosphate, other bioceramics, polymeric scaffolds and metallic implants are examples of biomaterials widely utilized in bone and hard tissue engineering 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Many efforts have been made to introduce green and safe reducer to overcome this limitation of hydrazine. For example, protein like bovine serum albumin (BSA) and ascorbic acid are the safe reducers for GO 3 , 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

Bovine serum albumin-functionalized graphene-decorated strontium as a potent complex nanoparticle for bone tissue engineering

Akbari

Askari

Naghib

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Graphene and its family have a great potential in tissue engineering because of their super mechanical properties, electrical conductivity and antibacterial properties. Considering other properties of graphene such as high surface area and ready-to-use functionalization according to the high oxygen-containing groups in graphene oxide family, some needs could be addressed in bone tissue engineering. Herein, we synthesized and decorated strontium nanoparticles (SrNPs) during the reduction process of graphene oxide using green and novel method. Without using hydrazine or chemical linkers, strontium NPs were synthesized and decorated on the surface of rGO simultaneously using BSA. The results of the UV–Vis, FTIR and Raman spectroscopy demonstrated that BSA could successfully reduce graphene oxide and decorated SrNPs on the surface of rGO. FESEM and TEM exhibited that in situ synthesized SrNPs had 25–30 nm diameter. Interestingly, cell viability for MC3T3-E1 cells treated with SrNPs-rGO, were significantly higher than BSA-rGO and GO in constant concentration. Furthermore, we investigated the alkaline phosphatase activity (ALP) of these nanosheets that the results demonstrated Sr-BSA-rGO enhanced ALP activity more than GO and BSA-rGO. Remarkably, the relative expression of RUNX 2 and Col1 genes of MC3T3-E1 cells was boosted when treated with Sr-BSA-rGO nanosheets. This study revealed that using proteins and other biomolecules as green and facile agent for decoration of smart nanoparticles on the surface of nanosheets, would be promising and assist researcher to replace the harsh and toxic hydrazine like materials with bio-friendly method. These results demonstrated that Sr-BSA-rGO had the excellent capability for regenerating bone tissue and could be used as an osteogenesis booster in implants.

show abstract

Reduced graphene oxide-grafted bovine serum albumin/bredigite nanocomposites with high mechanical properties and excellent osteogenic bioactivity for bone tissue engineering

Cited by 23 publications

References 58 publications

Synthesis and investigation on microstructural, mechanical features of mesoporous hardystonite/reduced graphene oxide nanocomposite for medical applications

Synthesis and investigation on microstructural, mechanical features of mesoporous hardystonite/reduced graphene oxide nanocomposite for medical applications

Up-to-date progress in bioprinting of bone tissue

Bovine serum albumin-functionalized graphene-decorated strontium as a potent complex nanoparticle for bone tissue engineering

Contact Info

Product

Resources

About