Reduced HeLa cell viability in methionine‐containing cell culture medium irradiated with microwave‐excited atmospheric‐pressure plasma

Takahashi, Yohei; Taki, Yusuke; Takeda, Keigo; Hashizume, Hiroshi; Tanaka, Hiromasa; Ishikawa, Kenji; Hori, Masaru

doi:10.1002/ppap.201700200

Cited by 13 publications

(10 citation statements)

References 28 publications

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“…However, so far, most of the studies have examined the loss of function of amino acids as nutrients. For example, methionine in medium was oxidized by LTP treatment 27 . Ringer’s lactate solution contains sodium chloride, potassium chloride, calcium chloride, and L-sodium lactate, and we have previously reported that the reaction products between plasma and L-sodium lactate were anti-tumor factors in PAL 17 .…”

Section: Introductionmentioning

confidence: 99%

Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

Tanaka

Hosoi

Ishikawa

et al. 2021

Sci Rep

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Low-temperature plasma is being widely used in the various fields of life science, such as medicine and agriculture. Plasma-activated solutions have been proposed as potential cancer therapeutic reagents. We previously reported that plasma-activated Ringer’s lactate solution exhibited selective cancer-killing effects, and that the plasma-treated L-sodium lactate in the solution was an anti-tumor factor; however, the components that are generated through the interactions between plasma and L-sodium lactate and the components responsible for the selective killing of cancer cells remain unidentified. In this study, we quantified several major chemical products, such as pyruvate, formate, and acetate, in plasma-activated L-sodium lactate solution by nuclear magnetic resonance analysis. We further identified novel chemical products, such as glyoxylate and 2,3-dimethyltartrate, in the solution by direct infusion-electrospray ionization with tandem mass spectrometry analysis. We found that 2,3-dimethyltartrate exhibited cytotoxic effects in glioblastoma cells, but not in normal astrocytes. These findings shed light on the identities of the components that are responsible for the selective cytotoxic effect of plasma-activated solutions on cancer cells, and provide useful data for the potential development of cancer treatments using plasma-activated L-sodium lactate solution.

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Section: Introductionmentioning

confidence: 99%

Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

Tanaka

Hosoi

Ishikawa

et al. 2021

Sci Rep

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“…In addition, it is found that Met in proteins is highly susceptible to oxidation under oxidative stress. 15 − 19 As an antioxidant defense system, Met oxidation participates in regulation of protein stability 20 and cellular viability 21 through its own oxidation: Met is oxidized to form methionine sulfoxide(MetSO) by adding oxygen to its sulfur atom in the physiological environment. MetSO produced may easily be reduced back to Met by thioredoxin-dependent enzymes under catalysis of methionine sulfoxide reductase.…”

Section: Introductionmentioning

confidence: 99%

“…Met is not only a precursor in protein synthesis but also participates in many biochemical processes, ,,, such as glycogen and d -glucose formation, production of S -adenosylmethionine and Succinyl-CoA, biosynthesis of homocysteine, cysteine, and glutathione, metabolism of phosphatidylcholine and phospholipids, as well as cellular and DNA methylation. In addition, it is found that Met in proteins is highly susceptible to oxidation under oxidative stress. − As an antioxidant defense system, Met oxidation participates in regulation of protein stability and cellular viability through its own oxidation: Met is oxidized to form methionine sulfoxide(MetSO) by adding oxygen to its sulfur atom in the physiological environment. MetSO produced may easily be reduced back to Met by thioredoxin-dependent enzymes under catalysis of methionine sulfoxide reductase. , The impairment of this regulation is associated with many diseases such as lung disease, cancer, neurodegeneration (Alzheimer’s disease), and cardiovascular and cerebrovascular disease. , However, the regulation functions of methionine as an antioxidant are still not fully explored.…”

Section: Introductionmentioning

confidence: 99%

Autoinhibition by Iodide Ion in the Methionine–Iodine Reaction

Csekõ

Horváth

2020

J. Phys. Chem. A

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The methionine–iodine reaction was reinvestigated spectrophotometrically in detail monitoring the absorbance belonging to the isosbestic point of iodine at 468 nm, at T = 25.0 ± 0.1 °C, and at 0.5 M ionic strength in buffered acidic medium. The stoichiometric ratio of the reactants was determined to be 1:1 producing methionine sulfoxide as the lone sulfur-containing product. The direct reaction between methionine and iodine was found to be relatively rapid in the absence of initially added iodide ion, and it can conveniently be followed by the stopped-flow technique. Reduction of iodine eventually leads to the formation of iodide ion that inhibits the reaction making the whole system autoinhibitory with respect to the halide ion. We have also shown that this inhibitory effect appears quite prominently, and addition of iodide ion in the millimole concentration range may result in a rate law where the formal kinetic order of this species becomes −2. In contrast to this, hydrogen ion has just a mildly inhibitory effect giving rise to the fact that iodine is the kinetically active species in the system but not hypoiodous acid. The surprisingly complex kinetics of this simple reaction may readily be interpreted via the initiating rapidly established iodonium-transfer process between the reactants followed by the subsequent hydrolytic decomposition of the short-lived iodinated methionine. A seven-step kinetic model to be able to describe the most important characteristics of the measured kinetic curves is established and discussed in detail.

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“…Different amino acid combinations and concentrations in different media may considerably affect CAP efficacy due to potential reactions with plasma‐activated reactive species. It was reported that cysteine and methionine mainly contribute to the activity attenuation of plasma‐activated Dulbecco's modified Eagle's medium (DMEM) at 22°C and 8°C [ 80 ] ; the concentration of the essential amino acid methionine declined in PAM, whereas that of methionine sulfoxide increased due to methionine oxidation, and this contributes to the reduced survival rate of HeLa cervical carcinoma cells [ 81 ] ; and adding 20 mM lysine in DMEM could significantly increase the antiglioblastoma capacity of PAM. [ 82 ] These suggest that amino acids such as methionine, cysteine, and lysine are potential confounding factors of plasma dose if PAM is considered, and their contributions and effects vary in the treatment of different types of cancers.…”

Section: Challenges For Precision Plasma Dosementioning

confidence: 99%

Dosing: The key to precision plasma oncology

Dai

Zhang

et al. 2020

Plasma Processes & Polymers

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Cold atmospheric plasma (CAP) is an emerging oncotherapeutic approach with selectivity for cancer cells. It can induce different cell survival and death programs depending on the CAP dose, and it can function as a neo or adjuvant therapy against cancer. Establishing an evaluation system for precise CAP dosing is the key to make plasma therapy act alone or in combination with other therapeutic modalities for achieving desirable treatment responses. By classifying CAP-induced effects and associating them with the dosing of plasma-reactive agents, we identify opportunities for CAP to contribute to precision oncotherapy and discuss challenges en route to clinical applications. We emphasize the importance of dosing in plasma medicine and encourage crossdisciplinary collaborations to develop suitable dosing metrics.

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Reduced HeLa cell viability in methionine‐containing cell culture medium irradiated with microwave‐excited atmospheric‐pressure plasma

Cited by 13 publications

References 28 publications

Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

Autoinhibition by Iodide Ion in the Methionine–Iodine Reaction

Dosing: The key to precision plasma oncology

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