Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

Divari, Sara; Berio, Enrica; Biolatti, B.; Cannizzo, Francesca Tiziana

doi:10.1021/acs.jafc.7b04795

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…In recent years, increasing number of studies have been focused on the expression of target genes at different growth stages or in different organs. ,,, In the current study, CAP , EIF4A , and TUB were identified as suitable reference genes at different growth stages and TUB , 25S , EF1 , and EIF4A in different organs. Rankings of gene candidates by different algorithms were not identical; a similar phenomenon has been observed in E .…”

Section: Discussionmentioning

confidence: 88%

Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

Zhao

Yan

Wang

et al. 2018

J. Agric. Food Chem.

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Severe infestations of shortawn foxtail ( Alopecurus aequalis Sobol.), a noxious weed in wheat and canola cropping systems in China, remain standing even after the application of the herbicides, fenoxaprop- P-ethyl and mesosulfuron-methyl. Analysis of gene expression in weed plants subjected to herbicide treatment is a key step toward more mechanistic studies. Since such an analysis often involves quantitative real-time PCR (qRT-PCR), endogenous reference genes with stable expression are required. Herein, we identified specific gene sets, suitable as references for qRT-PCR data normalization in A. aequalis plants under different experimental conditions, using geNorm, NormFinder, BestKeeper, and RefFinder software. Additionally, the reliability of reference genes was verified by analyzing the expression of genes encoding two major herbicide target enzymes: acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS). Furthermore, functional ALS gene amplification was likely present in resistant plants, although it may make no contribution to the resistant phenotypes.

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Section: Discussionmentioning

confidence: 88%

Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

Zhao

Yan

Wang

et al. 2018

J. Agric. Food Chem.

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Gene expression profile associated with thymus regeneration in dexamethasone-treated beef cattle

Cannizzo

Cucuzza

Divari

et al. 2018

Domestic Animal Endocrinology

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Glucocorticoids (GCs) are illegally used as growth promoters in cattle, and the analytical methods officially applied most likely underestimate the precise frequency of the abuse. As a side effect, the administration of GCs causes fat infiltration, apoptosis, and atrophy of the thymus. However, gross and histological observations carried out previously showed that the thymus preserves an intrinsic ability to regenerate. The aim of this work was to study the transcriptional effects of GCs on genes likely involved in regeneration of the epithelial cell network in the cervical and thoracic thymus of beef cattle treated with dexamethasone (DEX) or prednisolone (PRD) in comparison with a control group. Moreover, the ratio of bax/bcl2 genes was examined to verify a possible antiapoptotic activity occurring at the same time. In the cervical thymus, DEX administration increased the gene expression of c-myc (P < 0.01), tcf3 (P < 0.05), tp63 (P < 0.01), and keratin 5 (krt5; P < 0.01). In the thoracic thymus of DEX-treated cattle, the gene expression of tcf3 (P < 0.01), tp63 (P < 0.01), and krt5 (P < 0.05) was increased. These results suggested that thymic regeneration is underway in the DEX-treated animals. However, the bax/bcl2 ratio was decreased in both cervical and thoracic thymus of DEX-treated cattle (P < 0.01 and P < 0.05, respectively), showing an antiapoptotic effect through the mitochondrial pathway. Conversely, PRD administration caused no change in the expression of all considered genes. These results sustain the hypothesis that regeneration occurs in the thymus parenchyma 6 d after the DEX treatment was discontinued. This hypothesis is also supported by the absence of alterations in the thymus of PRD-treated beef cattle. Indeed, previous studies showed the inability of PRD to induce macroscopic and microscopic lesions in the thymus. Therefore, in this context, it is not surprising that PRD induced no alteration of genes involved in the regeneration pathway.

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Analysis of stability of reference genes for qPCR in bovine preadipocytes during proliferation and differentiation in vitro

Wang

Zhang

et al. 2022

Gene

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Reference Gene Selection and Prednisolone Target Gene Expression in Adipose Tissues of Friesian Cattle

Cited by 4 publications

References 33 publications

Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

Gene expression profile associated with thymus regeneration in dexamethasone-treated beef cattle

Analysis of stability of reference genes for qPCR in bovine preadipocytes during proliferation and differentiation in vitro

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