Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (<i>Alopecurus aequalis</i> Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

Zhao, Ning; Yan, Yanyan; Wang, Hengzhi; Bai, Siqi; Wang, Qian; Liu, Weitang

doi:10.1021/acs.jafc.8b03054

Cited by 33 publications

(24 citation statements)

References 50 publications

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“…ALS expression was upregulated 15.5-to 21.0-fold after mesosulfuron-methyl treatment in shortawn foxtail (Alopecurus aequalis Sobol.) (Zhao et al 2018). Higher gene expression levels may lead to the accumulation of ALS isozymes, which can thus enhance the tolerance or resistance of weeds to herbicides by compensating for reduced ALS activity.…”

Section: Relative Expression Of Als1 and Als2 In Tr And Ts Descurainia Sophiamentioning

confidence: 99%

Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in flixweed (Descurainia sophia)

Shen

et al. 2021

Weed Sci

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Flixweed [Descurainia sophia (L.) Webb ex Prantl] is a notorious broadleaf weed that is widely distributed in winter wheat growing areas of China, and has evolved resistance to tribenuron-methyl mainly due to target-site resistance (TSR) mutations in acetolactate synthase (ALS). In the current research, two ALS genes were identified in tribenuron-methyl-susceptible (TS) or -resistant (TR) D. sophia. Resistance mutations of Asp-376-Glu and Pro-197-Ala were identified on ALS1 and ALS2 isozymes in TR D. sophia, respectively. The TR D. sophia evolved 10836.3-fold resistance to tribenuron-methyl and displayed cross-resistance to multiple ALS-inhibiting herbicides with different chemical structures. Dose response experiments and ALS activity assay indicated that two mutated ALS isozymes contributed differentially in resistance to tribenuron-methyl, flucetosulfuron and pyribenzoxim. In addition, the relative expression level of ALS1 gene was 2.2- and 1.6-fold higher than ALS2 genes in TR D. sophia on the 1st and 7th days after tribenuron-methyl treatment (DAT), respectively. In contrast, the relative expression level of ALS1 and ALS2 in TS D. sophia is similar. This is the first research that explored different roles of ALS isozymes in resistance to ALS-inhibiting herbicides, which might provide a new perspective for the weed resistance management.

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Section: Relative Expression Of Als1 and Als2 In Tr And Ts Descurainia Sophiamentioning

confidence: 99%

Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in flixweed (Descurainia sophia)

Shen

et al. 2021

Weed Sci

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“…Like for the ACCase gene, overexpression of the ALS gene is also rare (Zhao et al, 2018). In addition, ALS gene overexpression may be insufficient to provide resistance to ALS inhibitors (Wang et al, 2019).…”

Section: Resistance To Als Inhibitorsmentioning

confidence: 99%

Accumulation of Target Gene Mutations Confers Multiple Resistance to ALS, ACCase, and EPSPS Inhibitors in Lolium Species in Chile

et al. 2020

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“…Overexpression of the ALS gene has been reported in resistant shortawn foxtail (Alopecurus aequalis Sobol.) from Japan and China (Iwakami et al 2017;Zhao et al 2018).…”

Section: Determination Of Relative Expression Level Of Als Gene In Thmentioning

confidence: 99%

Non–target site based resistance to the ALS-inhibiting herbicide mesosulfuron-methyl in American sloughgrass (Beckmannia syzigachne)

Wang

Liu

et al. 2019

Weed Sci

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American sloughgrass [Beckmannia syzigachne (Steud.) Fernald] is one of the most predominant and troublesome weeds in wheat (Triticum aestivum L.) fields rotated with rice (Oryza sativa L.) in China. Mesosulfuron-methyl is one of the main herbicides used to selectively control B. syzigachne in winter wheat fields in China. After many years of application, mesosulfuron-methyl failed to control B. syzigachne in Yutai County. The objectives of this study were to determine the resistance level to mesosulfuron-methyl and other acetolactate synthase (ALS) inhibitors in the B. syzigachne population collected from Yutai County (R) and identify the mechanism of resistance. The results indicated that the R population was 4.1-fold resistant to mesosulfuron-methyl and was cross-resistant to pyroxsulam (600-fold), imazethapyr (4.1-fold), flucarbazone (12-fold), and bispyribac-sodium (12-fold). In vitro assays revealed that ALS in the R population was as sensitive as that in a susceptible (S) population. Gene sequence analysis identified no known resistant mutations in the ALS gene of the R population. Furthermore, real-time quantitative reverse transcriptase PCR experiments indicated that the expression level of the ALS gene in the R population was not different from that of the S population. However, the cytochrome P450 inhibitor malathion reversed the R population's resistance to mesosulfuron-methyl. The result of ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS-MS) spectral analysis indicated that the metabolic rates of mesosulfuron-methyl in the R population were significantly faster than in the S population. Therefore, non-target resistance to mesosulfuron-methyl has been demonstrated in the R population. The resistance was very likely caused by enhanced herbicide metabolism.

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Acetolactate Synthase Overexpression in Mesosulfuron-Methyl-Resistant Shortawn Foxtail (Alopecurus aequalis Sobol.): Reference Gene Selection and Herbicide Target Gene Expression Analysis

Cited by 33 publications

References 50 publications

Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in flixweed (Descurainia sophia)

Effects of a novel combination of two mutated acetolactate synthase (ALS) isozymes on resistance to ALS-inhibiting herbicides in flixweed (Descurainia sophia)

Accumulation of Target Gene Mutations Confers Multiple Resistance to ALS, ACCase, and EPSPS Inhibitors in Lolium Species in Chile

Non–target site based resistance to the ALS-inhibiting herbicide mesosulfuron-methyl in American sloughgrass (Beckmannia syzigachne)

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