Refined localization of the Escherichia coli F4ab/F4ac receptor locus on pig chromosome 13

Abstract

Diarrhoea in newborn and weaned pigs caused by enterotoxigenic Escherichia coli (ETEC) expressing F4 fimbriae leads to considerable losses in pig production. In this study, we refined the mapping of the receptor locus for ETEC F4ab/F4ac adhesion (F4bcR) by joint analysis of Nordic and Swiss data. A total of 236 pigs from a Nordic experimental herd, 331 pigs from a Swiss experimental herd and 143 pigs from the Swiss performing station were used for linkage analysis. Genotyping data of six known microsatellite markers, two newly developed markers (MUC4gt and HSA125gt) and an intronic SNP in MUC4 (MUC4-8227) were used to create the linkage map. The region for F4bcR was refined to the interval SW207-S0075 on pig chromosome 13. The most probable position of F4bcR was in the SW207-MUC4 region. The order of six markers was supported by physical mapping on the BAC fingerprint contig from the Wellcome Trust Sanger Institute. Thus, the region for F4bcR could be reduced from 26 to 14 Mb.

A Genome-Wide Association Study Identifies Two Novel Promising Candidate Genes Affecting Escherichia coli F4ab/F4ac Susceptibility in Swine

“…The positions of the 18 SNPs are consistent with previously reported QTL regions [3], [9]. These results offer a high possibility that F4abR and F4acR share the common causal mutation(s) as suggested by some previous studies [5], [7], [29].…”

“…Once the bacteria adhere to the brush border of enterocytes and colonize the small intestine, the enterotoxins produced by them induce an increased secretion of electrolytes into lumen, resulting in diarrhoea. However, not all piglets are susceptible to ETEC F4 and the susceptibility is determined by the existence of a specific receptor of ETEC F4ab/F4ac [3].…”

“…A linkage and comparative mapping study demonstrated that the most likely region of F4abR/F4acR gene(s) was between SW207 and S0075 [7]. Studies [3], [8], [9] suggested that the MUC4 (mucin 4) gene should be considered as one of the most promising candidate genes for F4abR/F4acR based on three aspects: (1) its physical position falls into the region identified in most of linkage analyses, (2) the protein encoded by the MUC4 gene is one of mucin-like sialoglycoprotein which the ETEC F4 maybe adheres to, and (3) association studies showed strong association between a mutation within the gene and the adhesive phenotypes. However, in a recent study, Rampoldi et al found that the region of F4abR/F4acR gene(s) was distal to the interval between the MUC4 gene and the LMLN gene (leishmanolysin-like gene) through testing recombination events in three–generation pedigree [10].…”

Association of B3GNT5 Polymorphisms with Susceptibility to ETEC F4ab/ac in the White Duroc × Erhualian Intercross and 15 Outbred Pig Breeds

“…The location of F4ab/acR was then refined to a region between Sw207 and S0075 on SSC13q41 in this population (Jørgensen et al 2003). More recently, F4ab/acR was narrowed to the SW207-S0075 interval on SSC13 and the most likely position was in the SW207-MUC4 region (Joller et al 2009). Those encoding mucin-like sialoglycoproteins and transferrin-like proteins (Erickson et al 1994;Grange and Mouricout 1996) in the refined region are most likely positional candidate genes.…”

Detection of quantitative trait loci for porcine susceptibility to enterotoxigenic Escherichia coli F41 in a White Duroc × Chinese Erhualian resource population

“…Moreover, several candidate genes (Joller et al, 2006;Peng et al, 2007;Zhang et al, 2008) for the F4ab/ac and F18 receptors (Meijerink et al, 2000) have been proposed. a These authors contributed equally to this study.…”