Regenerated spider silk as a new biomaterial for MEMS

Bai, Jiamei; Ma, Tim; Chu, Winnie Cw; Wang, Rizhi; Silva, Leandro; Michal, Carl A.; Chiao, J.-C.; Chiao, Mu

doi:10.1007/s10544-006-9820-x

Cited by 23 publications

(21 citation statements)

References 26 publications

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“…The E value of LDPE was the same as measured by Jee and co‐workers (Jee and Lee, ): 0.16 GPa and 0.19 GPa using the AFM‐based nanoindentation but different methods of evaluation. The E modulus of silkworm silk fibroin was different to literature data (Bai et al ., ), but these data were produced by classical nanoindentation. For comparison with data from plant‐produced spider silk fusion proteins we used measuring data from silkworm silk produced with the same method of AFM‐based nanoindentation with the same cantilevers.…”

Section: Resultsmentioning

confidence: 99%

Transglutamination allows production and characterization of native‐sized ELPylated spider silk proteins from transgenic plants

Weichert

Hauptmann

Menzel

et al. 2013

Plant Biotechnology Journal

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SummaryIn the last two decades it was shown that plants have a great potential for production of specific heterologous proteins. But high cost and inefficient downstream processing are a main technical bottleneck for the broader use of plant-based production technology especially for protein-based products, for technical use as fibres or biodegradable plastics and also for medical applications. High-performance fibres from recombinant spider silks are, therefore, a prominent example. Spiders developed rather different silk materials that are based on proteins. These spider silks show excellent properties in terms of elasticity and toughness. Natural spider silk proteins have a very high molecular weight, and it is precisely this property which is thought to give them their strength. Transgenic plants were generated to produce ELPylated recombinant spider silk derivatives. These fusion proteins were purified by Inverse Transition Cycling (ITC) and enzymatically multimerized with transglutaminase in vitro. Layers produced by casting monomers and multimers were characterized using atomic force microscopy (AFM) and AFM-based nanoindentation. The layered multimers formed by mixing lysine-and glutamine-tagged monomers were associated with the highest elastic penetration modulus.

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Section: Resultsmentioning

confidence: 99%

Transglutamination allows production and characterization of native‐sized ELPylated spider silk proteins from transgenic plants

Weichert

Hauptmann

Menzel

et al. 2013

Plant Biotechnology Journal

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“…[25,27,28,33,34,52,[75][76][77] Such decorated silk fibrils could be used as nanowires [51,78] or surface coatings. [79,80] Finally, spider-silk fibers could be applied in technical textiles (used for example in parachutes and bullet-proof vests) which demand high toughness in combination with sleaziness.…”

Section: Design Of New Proteins With Silk Featuresmentioning

confidence: 99%

Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Vendrely

Scheibel

2007

Macromolecular Bioscience

220

212

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The outstanding mechanical properties of spider silks have motivated many researchers to establish biotechnological production techniques which are necessary to provide sufficient amounts of silk proteins for industrial applications. Based on recent developments in genetic engineering, two strategies for the recombinant production of spider-silk proteins have been established which are discussed in detail. Further, protein-design strategies are described, enabling the combination of silk properties with additional biological, chemical, or technical features. We highlight the potential of engineered and recombinantly-produced spider-silk proteins to provide the basis for a new generation of biomaterials.

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“…These are referred to as nano-electro-mechanical systems (NEMS). The nano-carbons (graphene [1,2], carbon nanotubes [3,4], and fullerene [5]), biomolecules (proteins [6], lipids [7,8], and nucleic acids [9,10]), and metal nanoparticles [11,12] are examples of nanoscale components that have been integrated into MEMS or microfluidic devices. They provide a new functionality, better sensitivity, and higher reliability.…”

Section: Introductionmentioning

confidence: 99%

Tensile Strength of Silicon Nanowires Batch-Fabricated into Electrostatic MEMS Testing Device

et al. 2018

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Featured Application: Piezoresistive sensors, Torsional mirrors. Abstract:The tensile strength of a silicon nanowire (SiNW) that had been integrated into a silicon-on-insulator (SOI)-based microelectromechanical system (MEMS) device was measured using electrostatic actuation and sensing. SiNWs of about 150 nm diameter and 5 µm length were batch-fabricated into a 5-µm-thick SOI device layer. Since there was no interface between the SiNW and the MEMS device and the alignment was perfect, the SiNW integration into an SOI-MEMS was expected to be useful for developing highly sensitive biochemical sensors or highly reliable torsional mirror devices. The SiNW was tensile tested using the electrostatic MEMS testing device. The integration was achieved using a combination of anisotropic and an isotropic dry etching of silicon, with an inductively coupled plasma reactive ion etching. A fabricated silicon beam of 800 nm square was thinned by a sacrificial oxidation process. The tensile strength of the wire was 2.6-4.1 GPa, which was comparable to that of microscale silicon MEMS structures. The reliability of such a thin device was successfully verified for future applications of the device structures.

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Regenerated spider silk as a new biomaterial for MEMS

Cited by 23 publications

References 26 publications

Transglutamination allows production and characterization of native‐sized ELPylated spider silk proteins from transgenic plants

Transglutamination allows production and characterization of native‐sized ELPylated spider silk proteins from transgenic plants

Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Tensile Strength of Silicon Nanowires Batch-Fabricated into Electrostatic MEMS Testing Device

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