Regeneration and micrografting of lentil shoots

Gulati, Anju; Schryer, Pat; McHughen, Alan

doi:10.1007/s11627-001-0132-9

Cited by 33 publications

(15 citation statements)

References 17 publications

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“…AAA, and Uniola paniculata, (Bairu et al 2007(Bairu et al , 2008Valero-Aracama et al 2010) Conventional method for in vitro propagation such as micrografting appears to be a promising means in regenerating in vitro shoots. Grafting has been successfully used in the regeneration protocol for many species that are otherwise difficult to root in vitro, including Lens culinaris, Persea americana, Phaseolus polyanthus, and Lathyrus sativus (Gulati et al 2001;Raharjo and Litz 2004;Zambre et al 2001Zambre et al , 2002. In this study, preliminary experiments using elongated shoots grafted onto rootstock of B. sinuspersici cuttings suggested grafting as a promising option for increasing the efficiency of the regeneration procedure because many of the grafted plants remained viable for over a month (Fig.…”

Section: Discussionmentioning

confidence: 90%

Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Northmore

Zhou

Chuong

2011

Plant Cell Tiss Organ Cult

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Direct organogenesis provides an efficient means of propagating plants in vitro without the necessity of growing callus cultures. Shoot regeneration was achieved from bud explants of Bienertia sinuspersici on Murashige and Skoog's media supplemented with 6-benzyladenine (BA). The frequency of regeneration varied based on the concentration of BA used. The highest rate of new shoot induction was observed when explants were cultured on media containing 4.4 lM BA after three weeks. Shoots were successfully elongated on media containing gibberellic acid with the highest shoot elongation observed at 2.9 lM. Elongated shoots developed adventitious roots in media without plant growth regulator and were subsequently transplanted to soil. Light microscopic analysis of cross sections of leaves from regenerated shoots showed similar intracellular cytoplasmic compartmentalization of organelles in chlorenchyma cells compared to those observed in leaves grown under greenhouse conditions. Moreover, Western blot analysis detected high expression of key C 4 photosynthetic enzymes, suggesting the C 4 cycle is maintained in the single cell system. We have developed a procedure for whole plant regeneration via direct organogenesis in B. sinuspersici.

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Section: Discussionmentioning

confidence: 90%

Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Northmore

Zhou

Chuong

2011

Plant Cell Tiss Organ Cult

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“…However, micrografting was more effective for sunflower plant recovery from regenerated shoots, as the graft survival was much higher than the rooting efficiency, regardless of the regeneration regime (Table 3). Micrografting was used to recover regenerated shoots of citrus (Citrus sinensis; Peña et al 1995a, b), cotton (Gossypium hirsutum; Luo and Gould 1999), lentil (Lens culinaris; Gulati et al 2001), and king protea (Protea cynaroides; Wu et al 2007), in which rooting was also problematic. Shoots from sunflower leaf tissues were also successfully micrografted to form whole plants (Zhang and Finer 2015).…”

Section: Discussionmentioning

confidence: 99%

Use of cytokinin pulse treatments and micrografting to improve sunflower (Helianthus annuus L.) plant recovery from cotyledonary tissues of mature seeds

Zhang

Finer

2016

In Vitro Cell.Dev.Biol.-Plant

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Regeneration of sunflower plants from tissue culture can be problematic due to inefficiencies in shoot elongation and subsequent rooting. To minimize exposure to cytokinins, which can negatively impact shoot elongation and rooting, a cytokinin pulse treatment was evaluated by placing cotyledonary tissues from sunflower seeds onto a shoot induction medium containing 1.5 mg L −1 6-benzylaminopurine and 0.2 mg L −1 1-naphthaleneacetic acid for 0, 2, 4, 8, 12, and 16 d, prior to transfer to an elongation medium containing 0.1 mg L −1 gibberellic acid. The 16-d pulse treatment gave the highest numbers of shoot primordia, but shoot development was minimal and only 0.4 well-developed shoots could be obtained per explant. The 4-d pulse treatment followed by 14 d of culture on elongation medium led to the production of 5.5 well-developed shoots per explant. For plant recovery, well-developed shoots obtained with the 4-d pulse treatment followed by a 14-d elongation treatment were micrografted onto in vitro seedlings, with graft survival of over 60%. Short pulse treatments (4-and 8-d), followed by culture on elongation medium, generated well-developed shoots that seemed to be more responsive to micrografting than shoots obtained with the 16-d pulse treatment. The use of the 4-d pulse treatment with micrografting of well-developed shoots significantly improved the efficiency of whole-plant recovery of sunflower.

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“…Higher numbers of graft plants are possible because they establish from scions with one internode, whereas the successful rooting requires at least two nodes (one node for rooting). Under ideal conditions, up to five grafts could be made from a donor plant (three was more (Gulati et al 2001) compared to rooted cutting methods that can take 6 wk (Polanco and Ruizz 1977).…”

Section: Comparison Of Rooted Cutting Vs Graftingmentioning

confidence: 99%

“…In our preliminary studies with common bean, we consistently observed that rooting of cuttings took more time than graft establishment. Rooting problems in other pulse crops such as lentil have been overcome with micrografting techniques; Gulati et al (2001) experienced 96% efficiency with this technique and reported that lentil grafts established 4 wk earlier than rooted cuttings.…”

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confidence: 99%

Grafting as a tool in common bean breeding

Gurusamy

Bett²,

Vandenberg³

2010

Can. J. Plant Sci.

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. 2010. Grafting as a tool in common bean breeding. Can. J. Plant Sci. 90: 299Á 304. Compatibility of rootstock for grafting was investigated in four species of Phaseolus to study the use of grafting as a tool in bean breeding programs. Four genotypes of Phaseolus vulgaris and one genotype of P. coccineus were used as rootstocks. Two genotypes of P. vulgaris, and one each of P. acutifolius and P. angustissimus, and an interspecific hybrid of P. acutifolius )P. angustissiumus were used as scions. The common bean genotype ICA Pijao, a widely used female parent in bean interspecific hybridization, was the most compatible rootstock among the five genotypes tested, with high mean percent survival for scions across all four Phaseolus species. Grafting was an efficient technique compared with rooted cuttings for seed increase in determinate type bean plants, where obtaining the maximum number of clones before the reproductive phase is critical. Grafting resulted in 91 and 66% higher mean seed yield per plant compared with ungrafted control and rooted cutting treatments, respectively. This grafting technique using compatible rootstocks such as P. vulgaris ICA Pijao has potential to improve early generation seed increase ratio, multiplication of clones for screening, and perpetuation of sterile or recalcitrant interspecific hybrids for various breeding strategies. La greffe est une technique efficace comparativement a`l'usage de boutures racine´es pour la multiplication des semences de varie´te´s de haricot non remontantes, pour lesquelles il est capital d'obtenir un maximum de clones avant la reproduction. La greffe accroıˆt respectivement la production moyenne de semences de 91% et de 66% comparativement aux te´moins non greffe´s et aux boutures racine´es. La pre´sente technique, qui recourt a`des porte-greffes compatibles comme P. vulgaris ICA Pijao, pourrait rehausser le taux de multiplication des semences des premie`res ge´ne´rations, multiplier le nombre de clones en vue d'une pre´se´lection et perpe´tuer les hybrides interspe´cifiques ste´riles ou re´calcitrants dans le cadre de divers programmes d'ame´lioration ge´ne´tique.

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Regeneration and micrografting of lentil shoots

Cited by 33 publications

References 17 publications

Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Multiple shoot induction and plant regeneration of the single-cell C4 species Bienertia sinuspersici

Use of cytokinin pulse treatments and micrografting to improve sunflower (Helianthus annuus L.) plant recovery from cotyledonary tissues of mature seeds

Grafting as a tool in common bean breeding

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