Regeneration and protein profile analysis of callus transformant with geminivirus β-satellite fragment

Abstract

The β-satellite fragment is a single-strand DNA that has a size of 1.4 kb. This fragment plays role in pathogen-derived resistance [PDR]. The genetic transformation of the β-Satellite fragment carried out by the previous researcher is limited by the regeneration of transformant callus. Therefore, this research was aimed to find some factors affecting the regeneration of transformant and non-transformant calli. Furthermore, protein analysis was performed on both calli to identify any specific fragment differentiating both proteins. SDS-PAGE analysis showed 5 and 8 fragments from transformant and transformant respectively. One of the 5 fragments has a size of 13.32 kDa which was predicted as C1 protein. These results proved that the β-Satellite fragment has been successfully inserted and expressed in the transformant calli. Subsequent research is suggested to identify some differential fragments produced by both transformant and non-transformant.