Regeneration of the esophagus using gastric acellular matrix: an experimental study in a rat model

Urita, Yasuhisa; Komuro, Hiroaki; Chen, Guoping; Shinya, Miki; Kaneko, Sawa; Kaneko, Michio; Ushida, Takashi

doi:10.1007/s00383-006-1799-0

Cited by 64 publications

(32 citation statements)

References 11 publications

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“…Traditionally, two main classes of biomaterials have been utilized for the engineering of hollow organs; acellular matrices derived from donor tissues (3)(4)(5)(6)(7)(8), (e.g., bladder submucosa (lamina propria) and small intestinal submucosa), and synthetic polymers such as polyglycolic acid (PGA) (9,10), polylactic acid (PLA), and poly(lactic-co-glycolic acid) (PLGA). These materials have been tested in respect to their biocompatibility in the host tissues (11,12).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, synthetic polymers can be manufactured reproducibly on a large scale with controlled properties of their strength, degradation rate and ultrastructure (26,27). Both classes of biomaterials have been used either with or without cells for the tissue engineering of hollow organs and tissues, including the bladder (5, 6, 10), urethra (3,4,9), ureter (7), esophagus (8,28), intestine (28), uterus (29), vagina (29,30) and blood vessels (31,32).…”

Section: Introductionmentioning

confidence: 99%

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Composite scaffolds for the engineering of hollow organs and tissues

Eberli¹,

Filho²,

Atala³

et al. 2009

Methods

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Composite scaffolds for the engineering of hollow organs and tissues AbstractSeveral types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. This scaffold system may be useful in patients requiring hollow organ replacement. Title: COMPOSITE SCAFFOLDS FOR THE ENGINEERING OF HOLLOW ORGANS AND TISSUES Authors Abstract:Several types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. Thi...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Composite scaffolds for the engineering of hollow organs and tissues

Eberli¹,

Filho²,

Atala³

et al. 2009

Methods

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“…La constitution d'un épithélium kératinisé était complète 2 semaines après l'implantation. Il n'y avait pas de sténose ou de dilatation [85]. Badylak [94].…”

Section: Méthodesunclassified

Esophageal tissue engineering

Luc

Durand

Collet

et al. 2014

Expert Review of Medical Devices

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Decellularized matrixes (DM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical procedure of the esophagus is often complicated by stricture, but preclinical studies have shown that the use of a DM can mitigate stricture and promote a constructive outcome.Recognizing the potential benefits of DM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of DM from porcine esophagus.The developed protocol for esophageal DM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a good mechanical behavior. Stem cells remained viable when seeded upon the esophageal DM in vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.

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“…Not only reepithelization but also muscle formation was discovered in the cell-seeded SIS after implantation in animal body for 8 weeks [19]. Urita et al used the decellularized stomach tissue to evaluate the esophageal mucosa regeneration [13]. Isch et al implanted a commercial decellularized product, AlloDerm® (LifeCell TM ), for the esophagoplasty of canine cervical esophagus.…”

Section: Natural Biomaterialsmentioning

confidence: 99%

Tissue Engineering of Esophagus

Zhu¹,

Zhou²,

Hou³

2017

Esophageal Abnormalities

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The incidences of esophageal diseases like atresia, tracheoesophageal fistula, esophagitis, and even carcinoma rise rapidly worldwide. Traditional therapies such as surgery, chemotherapy, or/and radiotherapy, etc. always meet problems, leading to deterioration of the patients' life quality and sometimes the reduced survival rate. Tissue-engineered esophagus, a novel biologic substitute with tissue architecture and bio-functions, has been believed to be a promising replacement in the future. However, the research of esophageal tissue engineering is still at the early stage. Considerable research has been focused on the issues of developing ideal scaffolds with optimal materials and fabrication methods. The in vivo tests and clinic attempts are being progressed.

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Regeneration of the esophagus using gastric acellular matrix: an experimental study in a rat model

Cited by 64 publications

References 11 publications

Composite scaffolds for the engineering of hollow organs and tissues

Composite scaffolds for the engineering of hollow organs and tissues

Esophageal tissue engineering

Tissue Engineering of Esophagus

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