Regional Distribution of Neuropeptide Y and Its Receptor in the Porcine Central Nervous System

Abstract

The regional distribution of neuropeptide Y (NPY) immunoreactivity and receptor binding was studied in the porcine CNS. The highest amounts of immunoreactive NPY were found in the hypothalamus, septum pellucidum, gyrus cinguli, cortex frontalis, parietalis, and piriformis, corpus amygdaloideum, and bulbus olfactorius (200-1,000 pmol/g wet weight). In the cortex temporalis and occipitalis, striatum, hippocampus, tractus olfactorius, corpus mamillare, thalamus, and globus pallidus, the NPY content was 50-200 pmol/g wet weight, whereas the striatum, colliculi, substantia nigra, cerebellum, pons, medulla oblongata, and medulla spinalis contained less than 50 pmol/g wet weight. The receptor binding of NPY was highest in the hippocampus, corpus fornicis, corpus amygdaloideum, nucleus accumbens, and neurohypophysis, with a range of 1.0-5.87 pmol/mg of protein. Intermediate binding (0.5-1.0 pmol/mg of protein) was found in the septum pellucidum, columna fornicis, corpus mamillare, cortex piriformis, gyrus cinguli, striatum, substantia grisea centralis, substantia nigra, and cerebellum. In the corpus callosum, basal ganglia, corpus pineale, colliculi, corpus geniculatum mediale, nucleus ruber, pons, medulla oblongata, and medulla spinalis, receptor binding of NPY was detectable but less than 0.5 pmol/mg of protein. No binding was observed in the bulbus and tractus olfactorius and adenohypophysis. In conclusion, immunoreactive NPY and its receptors are widespread in the porcine CNS, with predominant location in the limbic system, olfactory system, hypothalamoneurohypophysial tract, corpus striatum, and cerebral cortex.

First survey and functional annotation of prohormone and convertase genes in the pig

“…NPY was over-expressed ( P -value < 8.1 × 10–4, fold change = 7.94) in neuroblasts relative to non-neuronal cells in GSE16855. This result is consistent with reports that the olfactory bulb exhibit high levels of immunoreactive NPY in the brain of pigs [65] and that NPY may inhibit excitatory neurotransmission in the rat olfactory bulb [66]. VEGFC was over-expressed in neuroblasts relative to non-neuronal cells ( P -value < 1.5 × 10 -9 , fold change > 10) in experiment GSE16855.…”

The brain-pituitary-adipocyte axis: role of leptin in modulating neuroendocrine function.

“…Neuropeptide Y ( NPY) is a member of a family of pancreatic peptides, and it is widely distributed in the central nervous system ( CNS) and, in particular, the hypothalamus (Tatemoto, 1982;Busch-Sorensen et al, 1989). Immunocytochemical evidence demonstrated coexistence of NPY and norepinephrine in brain stem neurons that project to the hypothalamus.…”

Comparative characterization and autoradiographic distribution of neuropeptide Y receptor subtypes in the rat brain

“…Moreover, in situ hybridization studies have revealed that the distribution of NPY mRNA correlates rather well with that of NPY-likeimmunoreactive material (Terenghi et al, 1987;Chan-Palay et al, 1988). Many studies have demonstrated the existence of specific receptors for NPY and related peptides in mammalian brain (Chang et al, 1985(Chang et al, , 1986(Chang et al, , 1988Saria et al, 1985;Martel et al, 1986Martel et al, , 1987Martel et al, , 1988Martel et al, , 1990aInui et al, 1988Inui et al, , 1989Busch-Sorensen et al, 1989;Lynch et al, 1989;Sheikh et al, 1989a,b;Ohkubo et al, 1990;Rosier et al, 1990;Sheikh and Williams, 1990;Dumont et al, 1992;Quirion and Martel, 1992). Using anatomical methods, it was shown that NPY binding sites are concentrated mostly in the hippocampal formation, various cortical areas, the olfactory bulb, and the lateral septum.…”