Regulation of cellulase biosynthesis and secretion in fungi

Merivuori, Hannele; Siegler, Katherine; Sands, Jeffrey A.; Montenecourt, Bland S.

doi:10.1042/bst0130411

Cited by 43 publications

(20 citation statements)

References 14 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The synthesis of various catabolic enzymes in micro-organisms is repressed when glucose or other readily metabolizable compounds are added to the culture. In Trichoderma viride (Nisizawa et al, 1972) and Trichoderma reesei (Merivouri et al, 1985) the formation of some hydrolases, including a-amylase, P-glucosidase, xylanase and cellulase, was affected by glucose. This phenomenon has been called the glucose effect (Epps & Gale, 1942), or catabolic repression (Neidhardt, 1960;Holzer, 1976).…”

Section: Discussionmentioning

confidence: 99%

Regulation of chitinase synthesis in Trichoderma harzianum

Ulhôa

Peberdy

1991

Journal of General Microbiology

102

View full text Add to dashboard Cite

The production of chitinase by Trichoderma species is of interest in relation to their use in biocontrol and as a source of mycolytic enzymes. Fourteen isolates of the genus were screened to identify the most effective producer of chitinase. The best strain for chitinase was Trichoderma hurziunum 39.1, and this was selected for study of the regulation of enzyme synthesis. Washed mycelium of T. hurziunum 39.1 was incubated with a range of carbon sources. Chitinase synthesis was induced on chitin-containing medium, but repressed by glucose and Nacetylglucosamine. Production of the enzyme was optimal at a chitin concentration of 0.5 %, at 28 "C, pH 6.0 and was independent of the age of the mycelium. The synthesis of chitinase was blocked by both 8-hydroxyquinoline and cycloheximide, inhibitors of RNA and protein synthesis, respectively. The mode of chitinase synthesis in this fungus is discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Regulation of chitinase synthesis in Trichoderma harzianum

Ulhôa

Peberdy

1991

Journal of General Microbiology

102

View full text Add to dashboard Cite

show abstract

“…al ., 1984), and 0-glycosylation appears to be obligatory for their secretion (Merivuori et al, 1984;Messner & Kubicek, 1988;Kubicek et al, 1987). We have recently shown that 0-glycosylation in T. reesei (Kruszewska et al, 1989) proceeds in a similar way to that in yeasts (Tanner & Lehle, 1987), and may be controlled at the level of Dol-P or GDP-mannose supply.…”

Section: Introductionmentioning

confidence: 98%

“…However, during growth on this carbon source, mycelia cannot be separated from the insoluble cellulose, and hence preparation of mycelial subcellular fractions is difficult. In the present study we have therefore made use of a soluble carbon source (lactose) that is metabolized only slowly by T. reesei; this relieves the fungus from carbon catabolite repression and allows secretion of cellulases (Merivuori et al, 1984). Controls using glycerol (which does cause catabolite repression) as carbon source were included.…”

Section: Stimulation Of T Reesei Cellulase and Protein Secretion Bymentioning

confidence: 99%

Stimulation of exoprotein secretion by choline and Tween 80 in Trichoderma reesei QM 9414 correlates with increased activities of dolichol phosphate mannose synthase

Kruszewska

Palamarczyk

Kubicek

1990

Journal of General Microbiology

View full text Add to dashboard Cite

~Addition of choline (20 mM) or Tween 80 (0.06 %) to the culture medium of Trichoderma reesei QM 9414 increased (a) the secretion of protein under both carbon-catabolite-repressed and -derepressed conditions, and (b) cellulase secretion under carbon-catabolite-derepressed conditions. In contrast, no stimulation by choline or Tween 80 was observed with the hypersecretory strain T. reesei RUT C-30. In view of the obligatory role of 0-glycosylation in protein secretion by this fungus, an investigation was made into the effects on this process of choline and Tween 80.A membrane preparation was isolated from both strains of T. reesei and used to assay enzymes involved in 0-glycosylation. Significant differences were observed with respect to the activity of dolichol phosphate mannose (Dol-P-Man) synthase only. Strain QM 9414, grown on media supplemented with choline or Tween 80 exhibited a two-to threefold higher activity of Dol-P-Man synthase compared to a control lacking these supplements. This stimulatory effect was observed during growth under both carbon-catabolite-repressed and -derepressed conditions. In contrast, strain RUT C-30 exhibited decreased activities of Dol-P-Man synthase when grown in media supplemented with choline. Choline had no effect on Dol-P-Man synthase in vifro, whereas Tween 80 decreased the activity. Thus the effect of Tween 80 or choline on protein secretion by T. reesei may be due to a stimulation of formation and/or activity of Dol-P-Man synthase, thereby elevating the level of 0-glycosylation and protein secretion.

show abstract

“…We have recently presented evidenice that low levels of a conidial endoglucanase and a plasma membrane-boundglucosidase are involved in cellulase induction in T. reesei QM9414 (15). The latter enzyme has been shown to form sophorose-the putative in vivo inducer (8,15,20,21,22,26,34,35)-in vitro (8). The mechanism of carbon catabolite repression has not been dealt-with; it has only been shown that it is not restricted to the presence of glucose but can be observed with several other sugars or glucose metabolites (25).…”

mentioning

confidence: 99%

Differential regulation of synthesis of multiple forms of specific endoglucanases by Trichoderma reesei QM9414

1988

View full text Add to dashboard Cite

A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucanase I by Trichoderma reesei. Five forms appeared upon growth on cellulose, whereas four and only two appeared upon growth on lactose (carbon catabolite derepression) and induction by sophorose in a resting cell system, respectively. All endoglucanases detected resembled endoglucanase I in their specificity, since they exhibited no activity toward xylan or paranitrophenyl-,-D-lactobioside. A small (25-kiodalton) endoglucanase only appeared during growth on cellulose. None of the multiple forms arose by postsecretional modification. The results indicate that sophorose may not be the only compound mediating cellulose induction of the specific endoglucanases in T. reesei.

show abstract

Regulation of cellulase biosynthesis and secretion in fungi

Abstract: press 23 1 61 -64 400 408 Microhiol. 34, 777 782 289-301 Vol. 13

Cited by 43 publications

References 14 publications

Regulation of chitinase synthesis in Trichoderma harzianum

Regulation of chitinase synthesis in Trichoderma harzianum

Stimulation of exoprotein secretion by choline and Tween 80 in Trichoderma reesei QM 9414 correlates with increased activities of dolichol phosphate mannose synthase

Differential regulation of synthesis of multiple forms of specific endoglucanases by Trichoderma reesei QM9414

Contact Info

Product

Resources

About