Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources

Içgen, Yasemin; İçgen, Bülent; Özcengiz, Gülay

doi:10.1016/s0923-2508(02)01366-9

Cited by 32 publications

(21 citation statements)

References 12 publications

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“…Several authors have used molasses as a carbon source in a culture medium for growth and sporulation of Bacillus spp. strains [40][41][42]. As it was decided to administer spores from this bacterium to experimental hives, an economical culture medium was assayed in order to obtain a high and profitable number of spores.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effects of Bacillus subtilis subsp. subtilis Mori2, a Honey-Associated Strain, on Honeybee Colony Performance

Sabaté

Cruz

Benítez-Ahrendts

et al. 2011

Probiotics & Antimicro. Prot.

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A Bacillus spp. strain isolated from a honey sample in Morillos (Salta, Argentina) was phylogenetically characterized as B. subtilis subsp. subtilis Mori2. The strain was administered to bee colonies as a monoculture in one litre of sugarcane syrup (125 g/L) at a final concentration of 10(5) spores/mL to evaluate the bee colony performance. The treated colony was monitored, and any changes were compared with the control hives. All conditions were identical (weather, nourishment and supervision), except for the Bacillus spore supplement. The new nourishment, which was administered monthly from May to December 2010, was accepted by the bees and consumed within ca. 24-48 h. Photograph records and statistic analyses revealed significant differences in the open and operculated brood areas between the treated and control groups. The status of the colony improved after the second administration of the Bacillus spores until the end of the experiment. A higher number of bees were counted in the treated groups (26% more than the control) with respect to the initial number. Furthermore, at the time of harvest, honey storage in the treated hives was 17% higher than in the control hives. In addition, spore counts of both Nosema sp. and Varroa sp. foretica in treated hives were lower than in the control hives. These results with experimental hives would indicate that B. subtilis subsp. subtilis Mori2 favoured the performance of bees; firstly, because the micro-organism stimulated the queen's egg laying, translating into a higher number of bees and consequently more honey. Secondly, because it reduced the prevalence of two important bee diseases worldwide: nosemosis and varroosis.

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Section: Discussionmentioning

confidence: 99%

Beneficial Effects of Bacillus subtilis subsp. subtilis Mori2, a Honey-Associated Strain, on Honeybee Colony Performance

Sabaté

Cruz

Benítez-Ahrendts

et al. 2011

Probiotics & Antimicro. Prot.

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“…Although other carbon sources, as sucrose and maltose, allowed the appropriate growth of some Bt varieties [10,19], glucose is considered by some authors as the most suitable carbohydrate for Bt biolarvicide production [19,24]. Substrate concentrations of 10 g/l have been used in most fermentation studies, although higher concentrations were tested in a few cases.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Bacillus thuringiensis var. israelensis growth on high glucose concentrations

Berbert-Molina

Prata

Pessanha

et al. 2008

J Ind Microbiol Biotechnol

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The kinetic and general growth features of Bacillus thuringiensis var. israelensis were evaluated. Initial glucose concentration (S0) in fermentation media varied from 10 to 152 g/l. The results afforded to characterize four morphologically and physiologically well-defined culture phases, independent of S0 values: Phase I, vegetative growth; Phase II, transition to sporulation; Phase III, sporulation; and Phase IV, spores maturation and cell lysis. Important process parameters were also determined. The maximum specific growth rates (microX,m) were not affected with S0 up to 75 g/l (1.0-1.1 per hour), but higher glucose concentrations resulted in growth inhibition by substrate, revealed by a reduction in microX,m values. These higher S0 values led to longer Phases III and IV and delayed sporulation. Similar biomass concentrations (Xm=15.2-15.9 g/l) were achieved with S0 over 30.8 g/l, with increasing residual substrate, suggesting a limitation in some other nutrients and the use of glucose to form other metabolites. In this case, with S0 from 30.8 to 152 g/l, cell yield (YX/S) decreased from 0.58 to 0.41 g/g. On the other hand, with S0=10 g/l growth was limited by substrate, and YX/S has shown its maximum value (0.83 g/g).

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“…So, this carbon source, used as low-cost and available substrate at an industrial scale, is readily used. This finding is promising since Içgen et al (2002) reported that the use of maltose, starch and dextrin did not improve B. thuringiensis crystal titers. On the other hand, they also demonstrated that soya bean was among the best nitrogenous substrates which supported an optimal toxin production (Içgen et al , 2002), which agree with our findings.…”

Section: Resultsmentioning

confidence: 68%

Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium

Khedher

Jaoua

Zouari

2013

Braz. J. Microbiol.

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In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L−1 starch, 30 g L−1 soya bean and 9 g L−1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.

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Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources

Cited by 32 publications

References 12 publications

Beneficial Effects of Bacillus subtilis subsp. subtilis Mori2, a Honey-Associated Strain, on Honeybee Colony Performance

Beneficial Effects of Bacillus subtilis subsp. subtilis Mori2, a Honey-Associated Strain, on Honeybee Colony Performance

Kinetics of Bacillus thuringiensis var. israelensis growth on high glucose concentrations

Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium

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