Regulation of Granulocyte Responses in the Blood and Peritoneal Cavity of CBA and B10 Mice During an Acute Inflammation

Abstract

The regulatory mechanisms that determine the course of an inflammation induced by an intraperitoneal injection of kaolin were investigated in Listeria-susceptible CBA and Listeria-resistant B10 mice. The magnitude of the granulocyte inflammatory response in the peritoneal cavity was high in B10 mice (area under the curve; AUC0-48 h: 210.9 x 10(6) granulocytes/mouse x h) and lower in CBA mice AUC0-48 h: 136.8 x 10(6) granulocytes/mouse x h), whereas the reverse was seen for the granulocyte response in the peripheral blood (AUC0-48 h: 30.5 and 80.7 x 10(6) granulocytes/mouse x h, respectively). With respect to the presence of humoral factors that affect the number of granulocytes in the circulation, sera of both mouse strains sampled 24 h after the kaolin injection had granulocytosis-inducing effect in CBA recipient mice and did not induce a response in the B10 recipient mice. This divergent sensitivity to serum factors inducing granulocytosis is consistent with the difference in the blood granulocyte response of B10 and CBA mice but does not explain the divergent inflammatory responses in the peritoneal cavity. Computer simulation showed that at least two factors must be taken into consideration to explain the differences in the inflammatory response, i.e., a factor regulating the release of granulocytes from the bone marrow and a factor governing the rate of granulocyte efflux from the site of inflammation.

HLA-DR Alleles are Associated with IDDM, but not with Impaired Neutrophil Chemotaxis in IDDM

“…In murine studies, Listeria-susceptible A/J mice demonstrated impaired neutrophil and macrophage mobilization compared with Listeria-resistant C57/BL10 mice (Gervais et al, 1984). In a congenic mouse study, in which kaolin was injected into the peritoneal cavities of B10 and CBA mice, the B10 mice responded with higher numbers of neutrophils in peripheral blood, but fewer accumulated in the peritoneal cavity, in comparison with CBA mice (Sluiter et al, 1987). The present study did not find impaired neutrophil chemotaxis of IDDM to be associated with the HLA-DR alleles tested.…”

“…Several studies in mice indicate that neutrophil responses to immunologic challenge are under genetic control (Gervais et al, 1984;Sluiter et al, 1987). In B10 mice, major histocompatibility complex H-2d haplotype has been noted to augment the neutrophil response to bacterial lipopolysaccharides (Marley et al, 1994).…”

Optimized localization of bacterial infections with technetium-99m labelled human immunoglobulin after protein charge selection

“…Time-dependent accumulation of both 99mTc-labelled protein charge-purified human immunoglobulin and 99mTc-labelled unpurified human immunoglobulin at sites of infection suggests a correlation between the accumulation of the tracer agent and bacterial growth or increased infiltration of leucocytes at the site of infection [21,22]. No distinction between these two variables can be achieved in this model since a high number of bacteria is accompanied by a high influx of phagocytic cells from the circulation.…”

Influence of etoposide and cyclophosphamide on the efficacy of cloxacillin and erythromycin in an experimental staphylococcal infection

“…In the short-term infection model, the dominant role of the granulocytes compared with the role of monocytes was explained by the more rapid migration of the former to the site of inflammation (5,6,9). At relatively high doses of antibiotics there was less or no difference between the granulocytopenic mice and controls with respect to the numbers of bacteria.…”