Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts

Cardoso, Laís Medeiros; Pansani, Taísa Nogueira; Costa, Carlos Alberto de Souza; Basso, Fernanda Gonçalves

doi:10.1007/s10103-022-03579-z

Cited by 8 publications

(7 citation statements)

References 44 publications

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“…However, cell viabilities were not statistically different among the control, PLA, and PLA-IC-Nf groups. Previous studies have demonstrated that the concentration of proanthocyanidins impacts cell viability . Correspondingly, a faster release of PA from PLA_PA-Nf samples compared to PLA-IC-Nf samples may result in decreased cell viability in this study.…”

Section: Results and Discussionmentioning

confidence: 62%

“…Previous studies have demonstrated that the concentration of proanthocyanidins impacts cell viability. 43 Correspondingly, a faster release of PA from PLA_PA-Nf samples compared to PLA-IC-Nf samples may result in decreased cell viability in this study. 75 μg/mL concentration was preferred based on our previous study evaluating the effect of PA on DPSC viability at different concentrations (data not shown).…”

Section: ■ Results and Discussionmentioning

confidence: 65%

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Proanthocyanidin/β-Cyclodextrin Inclusion Complex in Electrospun Polylactic Acid Nanofibers: Preparation, Characterization, and Effects on Dental Pulp Stem Cells

Ballikaya,

Babadag,

Tüzün

et al. 2024

ACS Appl. Polym. Mater.

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An innovative biomaterial is needed to improve the tissue response and treatment outcomes in vital pulp tissue. In this regard, proanthocyanidins (PAs) have the potential to be used as a bioactive content of a scaffold since they can regulate oxidative stress and induce mineralization. The aim of this study was to engineer biodegradable electrospun polymeric nanofibers (Nfs) to enable the sustained release of PAs through a β-cyclodextrin (β-CD) inclusion complex and to investigate the effects of Nf mats on viability and the odontogenic differentiation capability of dental pulp stem cells (DPSCs). Bare polylactic acid nanofibers (PLA-Nfs), as well as 75 μg/mL PA-incorporated PLA_PA-Nf, and PLA-IC-Nf mats, were fabricated through the electrospinning technique. The morphology, composition, and hydrophilicity of the Nfs were characterized by scanning electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy, and contact angle measurements, respectively. The release of PA from the fibers was monitored for 30 days at predetermined intervals. The inclusion complex enabled the electrospun PLA-IC-Nfs to demonstrate a more sustained release profile of PA compared to that of the PLA_PA-Nfs. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl assay, and the antibacterial effects against Staphylococcus aureus and Escherichia coli were investigated by counting the colony-forming units. To assess odontogenic differentiation; calcium deposition and alkaline phosphatase (ALP) activity, Alizarin red staining was measured on day 14 and 18, while the levels of expression of the osteocalcin, Runt-related transcription factor 2, dentin sialophosphoprotein (DSPP), and dentin matrix protein (DMP) genes were evaluated on day 18. As a result, the successfully developed PLA-IC-Nf exhibited effective antibacterial activity against S. aureus and E. coli, antioxidant activity, and increased hydrophilicity compared to Nfs without the inclusion complex. Although the PLA-IC-Nf showed initial cytotoxicity, it gradually decreased over time to levels comparable with those of DPSCs alone, allowing the cell culture to reestablish itself due to a more controlled release. PLA-IC-Nf samples promoted odontogenic differentiation of DPSCs by upregulating the DSPP, DMP-1, and ALP activity.

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Section: Results and Discussionmentioning

confidence: 62%

Section: ■ Results and Discussionmentioning

confidence: 65%

Proanthocyanidin/β-Cyclodextrin Inclusion Complex in Electrospun Polylactic Acid Nanofibers: Preparation, Characterization, and Effects on Dental Pulp Stem Cells

Ballikaya,

Babadag,

Tüzün

et al. 2024

ACS Appl. Polym. Mater.

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“…Tissue regeneration capacity of OPCs (as a crosslinking agent in Type I collagen membrane) and antibacterial properties have also been evaluated and it has been reported that 10% OPCs-Col membrane was non-toxic, and it stimulated the proliferation of L929 and MG-63 cells in the in vitro study. Cardoso et al ( 27 ) evaluated the cytotoxic and genotoxic effects of various concentrations (100, 50, 10, and 5 μg/mL) of grape seed extract (GSE) on human gingival fibroblasts (HGF). They reported that the highest GSE concentrations (100 and 50 μg/mL) were both genotoxic and cytotoxic on HGF and GSE, while GSE at concentrations of 10 and 5 μg/mL enhanced cell viability after a 24h period.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells

Ballikaya,

Babadag,

Oya San Keskin

et al. 2024

Acta Stomatol Croat

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Objective This study aimed to evaluate the biological effects of “proanthocyanidin” (PA), and “nisin” (Ni), on dental pulp stem cells (DPSCs) and LPS-induced DPSCs as well as their antimicrobial effects against S. aureus and E. coli . Materials and methods After characterization of DPSCs, cytotoxicity of PA and Ni on DPSCs were evaluated using a water-soluble tetrazolium salt (WST-1). The cytokines and chemokines released by DPSCs and the expression levels of IL-6, IL-8, and TNF alpha were detected with human Cytokine Array C5 and enzyme‐linked immunosorbent assay (ELİSA), respectively. The antibacterial activities of PA and Ni were tested using the drop plate method. Results PA at 75 μg/ml increased cell viability, decreased TNF-α expression of DPSCs, did not show any cytotoxic effects on LPS-induced DPSCs, and also showed a tendency to decrease TNF-α expression. PA at 75 μg/ml exhibited higher expressions of TIMP-2, OPG, IL-7, and IL-8 in LPS-induced DPSCs compared to DPSCs. Ni at 100 μg/ml decreased TNF-α expression in DPSCs with no cytotoxic effects. It provided increased cell viability and a downregulation trend of TNF-α expression in LPS-induced DPSCs. Both Ni and PA provided strong antibacterial effects against S. aureus . Ni at 200μg/ml had strong antibacterial effects against E. coli without affecting negatively the viability of both DPSCs and LPS-induced DPSCs and showed anti-inflammatory activity by decreasing TNF-α expression. PA provided strong antibacterial effects against E. coli at 200 μg/ml but affected DPSCs viability negatively. Conclusion PA and Ni at specific concentrations exhibited immunomodulatory activity on DPSCs and LPS-induced DPSCs without any cytotoxic effects and strong antibacterial effects on S. aureus .

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“…These plates with the cells were maintained in a CO 2 incubator at 37°C for 24 h. After this, the culture medium was replaced by a new medium without FBS (Gibco) containing (or not) 100 ng/mL of tumor necrosis factor alpha (TNF‐α, Sigma‐Aldrich), following the experimental groups described in Table 1. After cells were incubated for 24 h in contact with the inflammatory stimulus, the experimental protocols were performed 25–27 …”

Section: Methodsmentioning

confidence: 99%

“…After cells were incubated for 24 h in contact with the inflammatory stimulus, the experimental protocols were performed. [25][26][27]…”

Section: Cell Culturementioning

confidence: 99%

EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF‐α

Pansani

Basso

Cardoso

et al. 2023

J of Periodontal Research

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Objective This study assessed the metabolism of oral mucosal cells cultured on titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and exposed to tumor necrosis factor alpha (TNF‐α). Methods Fibroblasts or keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 100 ng/mL of TNF‐α for 24 h. Groups were established: G1: Ti (control); G2: Ti + TNF‐α; G3: Ti + EGF; and G4: Ti + EGF + TNF‐α. Both cell lines were evaluated for: viability (AlamarBlue®, n = 8); interleukin 6 and 8 (IL‐6, IL‐8) gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For keratinocytes cells, the matrix metalloproteinase type 3 (MMP‐3) was evaluated by qPCR (n = 5) and ELISA (n = 6). A 3‐D culture of fibroblasts was analyzed by confocal microscopy. The data were subjected to ANOVA analysis, α = 5%. Results Increased cell viability was observed in all groups compared with G1. Enhanced gene expression and synthesis of IL‐6 and IL‐8 by fibroblasts and keratinocytes in G2 and modulation of hIL‐6 gene expression in G4 was noted. Modulation of IL‐8 synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed enhanced gene expression of hMMP‐3. A 3‐D culture showed more cells in G3. Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed elongated morphology with intact cytoplasm. Conclusions EGF coating increases cell viability and modulates the response of oral cells exposed to an inflammatory stimulus.

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Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts

Cited by 8 publications

References 44 publications

Proanthocyanidin/β-Cyclodextrin Inclusion Complex in Electrospun Polylactic Acid Nanofibers: Preparation, Characterization, and Effects on Dental Pulp Stem Cells

Proanthocyanidin/β-Cyclodextrin Inclusion Complex in Electrospun Polylactic Acid Nanofibers: Preparation, Characterization, and Effects on Dental Pulp Stem Cells

The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells

EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF‐α

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