Regulation of T lymphocyte subpopulations in specific pathogen-free chickens following experimental fowl adenovirus-Ⅷinfection

Abstract

Two-day-old specific pathogen-free (SPF) chickens were divided into two groups. Group I was inoculated orally with fowl adenovirus Ⅷ (FAV-Ⅷ). Group II served as a negative control. Chickens were investigated at various days post-inoculation (dpi) by flow cytometric analysis for changes in T lymphocyte subpopulations in immune system and blood. In the thymus, CD3+T lymphocytes were increased at 25 dpi, with significant increases in the FAV infected noted at 1, 12, 20dpi (p<0.05). This was accompanied by a corresponding increase of CD4+ and CD8+ T lymphocytes. In the spleen, CD3+ and CD4+ T lymphocytes were increased significantly at 30 dpi (p<0.01) whereas CD8+ and TCR γ δ+ T lymphocytes were decreased at 1 (p<0.05), 30 dpi (p<0.01). An increase of CD3+, CD4+ and CD8+ T lymphocytes was noticed in peripheral blood, and accompanied by a decrease of TCR γ δ+ T lymphocytes. These results demonstrated that infection with FAV-Ⅷ causes significant fluctuations in T lymphocyte subpopulations in thymus, blood and spleen. It can be concluded that an infection with FAV-Ⅷ has profound effects on the immune system, especially on cell mediated immune competency.

Attenuation and molecular characterization of fowl adenovirus 8b propagated in a bioreactor and its immunogenicity, efficacy, and virus shedding in broiler chickens

“…Live-attenuated vaccines mimic natural infections and should induce cellular immunity [ 49 ]. High levels of CD3 T cells have been reported among chickens inoculated with FAdV 8 up to 25 dpi [ 50 ], but CD3+, CD4+, and CD8+ cells were induced up to 42 dpi in this trial. These findings were more remarkable in the liver, which was the primary organ of infection at day 35 pi, but also in the spleen and thymus at different time points.…”

“…The thymus, where T cells mature, plays an important role in the development of cellular immunity [ 50 ]. However, FAdV 4 reduced the percentage of CD4+ and CD8+ cells in the thymus [ 13 ].…”

Efficacy, humoral, and cell-mediated immune response of inactivated fowl adenovirus 8b propagated in chicken embryo liver cells using bioreactor in broiler chickens

“…The undulation in the CD3+ T-cell sub-population in the inoculated chickens is a sign of T-lymphocyte inducement, an essential requirement for overcoming viral infections [51]. Montanide 71VG induced high cellular immunity when coupled with inactivated avian influenza virus vaccine in chicken [52] and could have aided the inducement of CMI in this trial. The CD3+ protein complex was first identified by immunoprecipitation experiments using human T-cells [53] and is a defining feature of the T-cell lineage; therefore, anti-CD3 antibodies can be used effectively as T-cell markers [53].…”

A case of adenovirus infection complicated by gram-negative polymicrobial sepsis: A clinical and morphological observation

“…Китайские ученые в эксперименте (на цыплятах) показали, что заражение куриным АДВ VIII приводило к значительным колебаниям количества Т-лимфоцитов субпопуляции ВЖ, крови и селезенки, оказывая чрезвычайный эффект на состояние клеточного иммунитета [32]. Инволюция ВЖ показана на морфологи-ТЕРАПЕВТИЧЕСКИЙ АРХИВ 11, 2015 Аденовирусная инфекция ческом материале у пациентов с фатальной аденовирусной пневмонией [33].…”