Regulation of the amino acid permeases in nitrogen‐limited continuous cultures of the yeast <i>Saccharomyces cerevisiae</i>

Olivera, Hiram; González, Alicia; Peña, Antonio

doi:10.1002/yea.320091005

Cited by 13 publications

(3 citation statements)

References 26 publications

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“…From the screening, we also identified the histidine and methionine transporters Hip1 and Mup1. Unlike NCR-sensitive permeases that transport amino acids primarily for use as a source of nitrogen, Hip1 and Mup1 are though to transport specific amino acids for direct use in protein synthesis when the nitrogen conditions are favorable (39). HIP1 and MUP1 expression is induced by the SPS amino acid-sensing system and has been shown to be down-regulated in the presence of a poor nitrogen source, and this down-regulation requires the presence of Gap1 at the plasma membrane (5).…”

Section: Fig 4 Loss Of Bul1 and Bul2 Function Results In Vacuolar Dmentioning

confidence: 99%

Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast

Villers

Savocco

Szopinska

et al. 2017

Molecular & Cellular Proteomics

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Yeast cells, to be able to grow on a wide variety of nitrogen sources, regulate the set of nitrogen transporters present at their plasma membrane. Such regulation relies on both transcriptional and post-translational events. Although microarray studies have identified most nitrogen-sensitive genes, nitrogen-induced post-translational regulation has only been studied for very few proteins among which the general amino acid permease Gap1. Adding a preferred nitrogen source to proline-grown cells triggers Gap1 endocytosis and vacuolar degradation in an Rsp5-Bul1/2-dependent manner. Here, we used a proteomic approach to follow the dynamics of the plasma membrane proteome after addition of a preferred nitrogen source. We identified new targets of the nitrogen regulation and four transporters of poor nitrogen sources-Put4, Opt2, Dal5, and Ptr2-that rapidly decrease in abundance. Although the kinetics is different for each transporter, we found that three of them-Put4, Dal5, and Ptr2-are endocytosed, like Gap1, in an Rsp5-dependent manner and degraded in the vacuole. Finally, we showed that Gap1 stabilization at the plasma membrane, through deletion of Bul proteins, regulates the abundance of Put4, Dal5 and Ptr2.

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Section: Fig 4 Loss Of Bul1 and Bul2 Function Results In Vacuolar Dmentioning

confidence: 99%

Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast

Villers

Savocco

Szopinska

et al. 2017

Molecular & Cellular Proteomics

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“…The proton excess generated during amino acid synthesis must be pumped out of the cell to keep the internal pH constant, resulting in the strong acidification of the medium, which affects ammonium uptake and growth (WIAME et al 1985). It has been reported that the affinity of permeases for amino acids changes with the charge on the amino acid (OLIVEIRA et al 1993). It has been reported that the affinity of permeases for amino acids changes with the charge on the amino acid (OLIVEIRA et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Biomass production and secretion of hydrolytic enzymes are influenced by the structural complexity of the nitrogen source inFusarium oxysporumandAspergillus nidulans

Silva

Bertolini

Ernandes

2001

J. Basic Microbiol.

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The structural complexity of the nitrogen sources strongly affects biomass production and secretion of hydrolytic enzymes in filamentous fungi. Fusarium oxysporum and Aspergillus nidulans were grown in media containing glucose or starch, and supplemented with a nitrogen source varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids), peptides (peptone) and protein (gelatin). In glucose, when the initial pH was adjusted to 5.0, for both microorganisms, higher biomass production occurred upon supplementation with a nitrogen source in the peptide form (peptone and gelatin). With a close to neutrality pH, biomass accumulation was lower only in the presence of the ammonium salt. When grown in starch, biomass accumulation and secretion of hydrolytic enzymes (amylolytic and proteolytic) by Fusarium also depended on the nature of the nitrogen supplement and the pH. When the initial pH was adjusted to 5.0, higher growth and higher amylolytic activities were detected in the media supplemented with peptone, gelatin and casamino acids. However, at pH 7.0, higher biomass accumulation and higher amylolytic activities were observed upon supplementation with peptone or gelatin. Ammonium sulfate and casamino acids induced a lower production of biomass, and a different level of amylolytic enzyme secretion: high in ammonium sulfate and low in casamino acids. Secretion of proteolytic activity was always higher in the media supplemented with peptone and gelatin. Aspergillus, when grown in starch, was not as dependent as Fusarium on the nature of nitrogen source or the pH. The results described in this work indicate that the metabolism of fungi is regulated not only by pH, but also by the level of structural complexity of the nitrogen source in correlation to the carbon source.

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“…2). As the PMF is known to be involved in fungal amino acid transport (Kropf et al, 1984;Eddy & Hopkins, 1988;Olivera et al, 1993;Pokorný et al, 2004), the effect of uncoupler was tested. The degree of inhibition increased from about 10-30 % in dormant conidia to 80-90 % in germinated conidia, suggesting an increased requirement of PMF by amino acid permeases during germination.…”

Section: Discussionmentioning

confidence: 99%

Nutrient transport into germinating Trichoderma atroviride conidia and development of its driving force

et al. 2015

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The exit from dormancy and the start of growth should be preceded or at least accompanied by the uptake of nutrients. In this work we studied changes in the transport of several nutrients into Trichoderma atroviride conidia. Germination started with a short period of isodiametric growth (conidial swelling), followed by polarized growth (germ tube formation) after about 8 h at 26 8C. The onset of isodiametric growth required the presence of external both phosphate and nitrate. At the same time, an increased uptake of precursors of macromolecules and phospholipids ( 14 C-or 3 H-labelled valine, uracil, N-acetylglucosamine and choline) occurred. A low uptake of these precursors was observed also in non-germinating conidia. Concomitantly, this uptake developed an increased sensitivity to the uncoupler 3,39,49,5-tetrachlorosalicylanilide. Expression and activity of H + -ATPase started after completing isodiametric growth, suggesting that the proton-motive force (PMF) generated by H + -ATPase may be an accelerator of nutrient uptake and metabolism.

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Regulation of the amino acid permeases in nitrogen‐limited continuous cultures of the yeast Saccharomyces cerevisiae

Cited by 13 publications

References 26 publications

Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast

Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast

Biomass production and secretion of hydrolytic enzymes are influenced by the structural complexity of the nitrogen source inFusarium oxysporumandAspergillus nidulans

Nutrient transport into germinating Trichoderma atroviride conidia and development of its driving force

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