Regulation of the Biological Functions of Osteoblasts and Bone Formation by Zn-Incorporated Coating on Microrough Titanium

Abstract

To improve the biological performance of titanium implant, a series of Zn-incorporated coatings were fabricated on the microrough titanium (Micro-Ti) via sol-gel method by spin-coating technique. The successful fabrication of the coating was verified by combined techniques of scanning electron microscopy, surface profiler, X-ray diffraction, X-ray photoelectron spectroscopy, and water contact angle measurements. The incorporated zinc existed as ZnO, which released Zn ions in a sustained manner. The Zn-incorporated samples (Ti-Zn0.08, Ti-Zn0.16, and Ti-Zn0.24) efficiently inhibited the adhesion of both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria. The in vitro evaluations including cell activity, alkaline phosphatase (ALP), mineralization, osteogenic genes expressions (Runx2, ALP, OPG, Col I, OPN, and OC), and tartrate-resistant acid phosphatase, confirmed that Ti-Zn0.16 sample was the optimal one to regulate the proliferation or differentiation for both osteoblasts and osteoclasts. More importantly, in vivo evaluations including Micro-CT analysis, push-out test, and histological observations verified that Ti-Zn0.16 implants could efficiently promote new bone formation after implantation for 4 and 12 weeks, respectively. The resulting material thus has potential application in orthopedic field.

Effects of the micro–nano surface topography of titanium alloy on the biological responses of osteoblast

“…More importantly, in vivo evaluations verified that the treatment could efficiently promote new bone formation in early times . Shen et al found that the micro‐structure contributed to the expression levels of type I collagen (COLIA 1), osteopontin (OPN), and osteocalcin (OCN) genes of MG63 cells …”

Effect of Octacalcium-Phosphate-Modified Micro/Nanostructured Titania Surfaces on Osteoblast Response

“…Previous studies have indicated that the surface characteristics of implants play a critical role in determining the cell and tissue responses. 1,2 Surface characteristics such as surface topography, wettability, roughness, and composition have been reported to markedly modulate protein adsorption and cell proliferation and differentiation in vitro and influence bone-to-implant contact as well as bone regeneration in vivo. 3,4 Hence, to achieve a desirable integration ability of titanium implants with bones, various surface modifications have been adopted to enable the substrates with specific properties to improve the bioactivity, including plasma immersion ion implantation, 5 deposition of calcium phosphate coatings, 6 and immobilization of biomolecules.…”

Cecropin B loaded TiO2 nanotubes coated with hyaluronidase sensitive multilayers for reducing bacterial adhesion

“…4A & C) and free bacteria in medium (Fig. 4B & D) after culture for 4 h, 24 h and 72 h [44,45], respectively. For the adhered S. epidermidis (Fig.…”