Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies

Romaniuk, A; Malejczyk, Jacek; Kubicka, U; Hyc, Anna; Olszewski, Waldemar L.; Moskalewski, Stanisław

doi:10.1016/0966-3274(95)80032-8

Cited by 31 publications

(29 citation statements)

References 30 publications

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“…Early aggrecan loss was also observed after allogenic chondrocyte transplantation [8] . The pattern of matrix degradation resembled that of degenerative joint diseases.…”

Section: Discussionmentioning

confidence: 78%

“…Allogenic cartilage transplants may fail due to immunological rejection, resulting in the absorption of cartilage, which compromises the long-term viability of the tissue [8] . Cartilage is an avascular tissue and its ECM does not allow permeability of molecules greater than 60 kDa [8] .…”

Section: Discussionmentioning

confidence: 99%

“…Cartilage is an avascular tissue and its ECM does not allow permeability of molecules greater than 60 kDa [8] . Therefore, the long-term survival of allogenic transplants of whole cartilage fragments may be explained by sequestra tion of chondrocyte-associated histocompatibility antigens by the matrix, preventing immunization of the host.…”

Section: Discussionmentioning

confidence: 99%

“…Failure of transplanted cartilage or allogenic chondro-cytes is characterized by degradation of cartilage and immunological rejection. A major feature is the loss of aggrecan from the cartilage matrix [7,8] . Many studies have shown that degenerative joint diseases are characterized by cartilage ECM degradation, where loss of aggrecan, an aggregating proteoglycan, is an early event in the destruction of the articular cartilage [9,10] .…”

Section: Introductionmentioning

confidence: 99%

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Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro¹

Wang

Zhou

et al. 2008

Acta Pharmacologica Sinica

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Aim: Failure of transplanted cartilage or allogenic chondrocytes is attributed mainly to immunological rejection and cartilage degradation. A major feature is the loss of aggrecan from the cartilage matrix, primarily due to the action of the specific proteinases aggrecanase-1 and aggrecanase-2. The aim of this in vitro study was to determine whether the specific inhibition of aggrecanase-1 and aggrecanase-2 by RNAi would mitigate aggrecan loss from cultured chondrocytes. Methods: Expression plasmid vectors of shRNA targeting aggrecanase-1 and aggrecanase-2 were constructed and transfected into cultured rattus costochondral chondrocytes. The transfected cells were induced with interleukin-1β (IL-1β). Gene mRNA levels were analyzed by RT-PCR. Aggrecan and collagen II content were measured by immunohistochemistry and Western blotting. Results: As the chondrocytes underwent dedifferentiation, agggrecanase-1 increased significantly. The specific inhibition of aggrecanase-1 and aggrecanase-2 by RNAi had no negative effect on the morphology and growth velocity of the chondrocytes. The mRNA of aggrecanase-1 and aggrecanase-2 decreased significantly. The α-2-macroglobulin expression level was increased by the shRNA specific for aggrecanase-1. Other genes of the chondrocytic extracellular matrix were not affected. RNAi significantly increased the aggrecan and collagen II content of chondrocytes treated with IL-1β. Conclusion: The results suggest that inhibition of aggrecanase-1 and aggrecanase-2 by RNAi can mitigate aggrecan degradation, without interfering with chondrocytic gene phenotype recovery. RNAi technology can be a useful tool for studying degenerative processes in cartilage.

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“…Early aggrecan loss was also observed after allogenic chondrocyte transplantation [8] . The pattern of matrix degradation resembled that of degenerative joint diseases.…”

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro¹

Wang

Zhou

et al. 2008

Acta Pharmacologica Sinica

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“…Unfortunately, transplants of viable allogeneic cartilages or freshly isolated donor chondrocytes evoke a strong immune response in the recipients that practically eliminates their use in routine plastic surgery. 37,43,44 Because the factors regulating differentiation of mesenchymal precursor cells toward chondrogenesis are not well identified, differentiated autologous chondrocytes remain the only feasible starting material, despite the fact that the number of cells from biopsies of autologous elastic cartilage is usually insufficient.…”

Section: Discussionmentioning

confidence: 99%

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

Chalain

Phillips

Hinek

1999

J. Biomed. Mater. Res.

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Transplantation of isolated chondrocytes has long been acknowledged as a potential method for rebuilding small defects in damaged or deformed cartilages. Recent advances in tissue engineering permit us to focus on production of larger amounts of cartilaginous tissue, such as might be needed for reconstructive surgery of the entire auricle. In this report we describe modification of the basic techniques that lead to production of a large amount of elastic cartilage originated from porcine and human isolated chondrocytes. Small fragments of auricular cartilage were harvested from children undergoing ear reconstruction for microtia or extirpation of preauricular tags and from ears of juvenile pigs. Enzymatically isolated elastic chondrocytes were then agitated in suspension to form the chondronlike aggregates, which were further embedded in molded hydrogel constructs made of alginate and type I collagen augmented with kappa-elastin. The constructs were then implanted in nude mice and harvested 4 and 12 weeks after heterotransplantation. The resulting neocartilage closely resembled native auricular cartilage at the gross, microscopic, and ultrastructural levels. Immunohistochemistry and electron microscopy additionally confirmed that the newly produced cartilage contained the major components of the elastic cartilage-specific matrix, including collagen type II, proteoglycans, and well-assembled elastic fibers.

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Vascular cell adhesion molecule 1 ?CD106) on primary human articular chondrocytes: Functional regulation of expression by cytokines and comparison with intercellular adhesion molecule 1 (CD54) and very late activation antigen2

Kienzle

Kempis

1998

Arthritis & Rheumatism

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VCAM-1, ICAM-1, and VLA-2 are constitutively expressed by human articular chondrocytes. Expression is regulated by cytokines. As shown for other chondrocyte genes, IL-1beta/TNFalpha and TGFbeta antagonistically modulate the expression of adhesion molecules. VCAM-1 and ICAM-1 contribute to adhesion of T lymphocytes to chondrocytes, and may thus participate in host defense mechanisms during inflammatory joint conditions such as rheumatoid arthritis and after cartilage transplantation.

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Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies

Cited by 31 publications

References 30 publications

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro¹

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro¹

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

Vascular cell adhesion molecule 1 ?CD106) on primary human articular chondrocytes: Functional regulation of expression by cytokines and comparison with intercellular adhesion molecule 1 (CD54) and very late activation antigen2

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Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies

Cited by 31 publications

References 30 publications

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro1

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro1

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

Vascular cell adhesion molecule 1 ?CD106) on primary human articular chondrocytes: Functional regulation of expression by cytokines and comparison with intercellular adhesion molecule 1 (CD54) and very late activation antigen2

Contact Info

Product

Resources

About

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro¹

Effects of RNAi-mediated inhibition of aggrecanase-1 and aggrecanase-2 on rat costochondral chondrocytesin vitro¹