Relationship Among Antibiotic Resistance, Biofilm Formation and lasB Gene in Pseudomonas Aeruginosa Isolated from Burn Patients

Abstract

Background: Pseudomonas aeruginosa is a major cause of hospital-acquired infection due to its high antibiotic resistance and biofilm formation ability. P. aeruginosa produces elastase lasB during biofilm formation, which can influence properties of biofilm. This study was carried out to evaluate the antibiotic resistance and distribution of the lasB gene among biofilm-producing P. aeruginosa strains isolated from burn patients. Methods: A total of 128 clinical samples were collected from burn patients. The P. aeruginosa isolates were identified using standard bacteriological procedures. Antibiotic susceptibility test was performed by the disk diffusion method. Biofilm formation was measured by microtiter plate assay. The presence of lasB gene was detected by PCR. Results: A total of 75 samples were positive for P. aeruginosa. A high rate of resistance was seen against ceftriaxone, cefotaxime, and piperacillin/tazobactam. Biofilm formation was seen in 57.3% of the isolates and the prevalence of the lasB gene was 85.3%. Biofilm formation in isolates without lasB was lower and these isolates were more sensitive to imipenem and piperacillin/tazobactam. Conclusions: In the present study, we did not find a statistically significant relationship among elastase gene (lasB) presence, antibiotic resistance, and biofilm formation in P. aeruginosa strains isolated from burn patients.

Groundwater, soil and compost, as possible sources of virulent and antibiotic-resistant Pseudomonas aeruginosa

“…Regarding extracellular protease encoding lasB (elastase LasB) and aprA (alkaline protease AprA), lasB was detected in a higher rate (100.0%) than in clinical settings (Roshani-Asl et al 2018). Due to this exclusiveness, it was not possible to evaluate the previously reported relationship between the presence of lasB gene and biofilm-forming ability or Imipenem, Piperacillin/Tazobactam resistance (Roshani-Asl et al 2018).…”

“…Regarding extracellular protease encoding lasB (elastase LasB) and aprA (alkaline protease AprA), lasB was detected in a higher rate (100.0%) than in clinical settings (Roshani-Asl et al 2018). Due to this exclusiveness, it was not possible to evaluate the previously reported relationship between the presence of lasB gene and biofilm-forming ability or Imipenem, Piperacillin/Tazobactam resistance (Roshani-Asl et al 2018). Regarding aprA, the previously reported clinical frequencies were 27.2% among patients with otitis media (Hassuna 2016), 28.6% among burned patients (Fazeli and Momtaz 2014) and 100% in intensive care units (ICUs) (Bradbury et al 2010).…”

Effects of human β-defensin 3 fused with carbohydrate-binding domain on the function of type III secretion system in Pseudomonas aeruginosa PA14

“…It indicated that the destruction of pseudomonas biofilm by antimicrobial peptides is not dependent on the effect of lasB. The role of lasB in the biofilm formation process is still controversial [31][32][33]. Thirdly, only the hBD3-CBD-treated PA14 ΔexsA group showed a lower hcnA expression.…”

Insights into Antagonistic Interactions of Multidrug Resistant Bacteria in Mangrove Sediments from the South Indian State of Kerala

“…Such variation within species would make it unreliable to establish a concentrate relationship on the biofilm formation and genus. Moreover, significant correlation was found between antibiotic resistance and biofilm formation in Pseudomonas aeruginosa [45,46] while other studies have found otherwise [47]. A study between P. aeruginosa from environmental and human sources observed significantly weaker biofilm formation and susceptibility to most antibiotics from environmental isolates as compared to human sources [48].…”