Relationship Between Intracellular Calcium, Energy Metabolism, and Motility of Ram Sperm

Breitbart, Haim; Nass-Arden, L

doi:10.3109/01485019508987858

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…Extracellular Ca 2+ strongly affects motility of intact hamster, mouse and rat sperm (Davis, 1978;Morton et al, 1978) and stimulates motility of hamster, bovine and human spermatozoa (Babcock et al, 1976;Lui & Meizel, 1979;Fakih et al, 1986). A relationship between intracellular calcium concentration, energy metabolism, and sperm motility was found in ram mitochondria (Breitbart & Nass-Arden, 1995). In this study, sperm motility dependent on mitochondrial activity was significantly inhibited by exogenous calcium, while glycolytic-dependent motility was unaffected under these conditions.…”

Section: Calcium and Sperm Mitochondriamentioning

confidence: 53%

“…, 1986). A relationship between intracellular calcium concentration, energy metabolism, and sperm motility was found in ram mitochondria (Breitbart & Nass‐Arden, 1995). In this study, sperm motility dependent on mitochondrial activity was significantly inhibited by exogenous calcium, while glycolytic‐dependent motility was unaffected under these conditions.…”

Section: Calcium and Sperm Mitochondriamentioning

confidence: 99%

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The role of mitochondria in energy production for human sperm motility

et al. 2011

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Mitochondria of spermatozoa are different from the corresponding organelles of somatic cells, in both their morphology and biochemistry. The biochemical differences are essentially related to the existence of specific enzyme isoforms, which are characterized by peculiar kinetic and regulatory properties. As mitochondrial energy metabolism is a key factor supporting several sperm functions, these organelles host critical metabolic pathways during germ cell development and fertilization. Furthermore, spermatozoa can use different substrates, and therefore activate different metabolic pathways, depending on the available substrates and the physico-chemical conditions in which they operate. This versatility is critical to ensure fertilization success. However, the most valuable aspect of mitochondria function in all types of cells is the production of chemical energy in the form of ATP which can be used, in the case of spermatozoa, for sustaining sperm motility. The latter, on the other hand, represents one of the major determinants of male fertility. Accordingly, the presence of structural and functional alterations in mitochondria from asthenozoospermic subjects confirms the important role played by these organelles in energy maintenance of sperm motility. The present study gives an overview of the current knowledge on the energy-producing metabolic pathways operating inside human sperm mitochondria and critically analyse the differences with respect to somatic mitochondria. Such a comparison has also been carried out between the functional characteristics of human sperm mitochondria and those of other mammalian species. A deeper understanding of mitochondrial energy metabolism could open up new avenues of investigation in bioenergetics of human sperm mitochondria, both in physiological and pathological conditions.

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Section: Calcium and Sperm Mitochondriamentioning

confidence: 53%

Section: Calcium and Sperm Mitochondriamentioning

confidence: 99%

The role of mitochondria in energy production for human sperm motility

et al. 2011

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“…Calcium has been implicated in sperm motion and function. 12,13 Nitric oxide is known to adversely affect sperm motion and viability, probably due to its capacity as a free radical inducing direct oxidative damage to the sperm membrane. 14 This effect of NO is concentration-dependent, however, as at low doses it is found to improve or maintain sperm motility, which might be connected to its role as a cGMP stimulant.…”

Section: Discussionmentioning

confidence: 99%

The effect of sildenafil on human sperm motion and function from normal and infertile men

et al. 2000

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The aim of this report was to study the effect of sildena®l, a speci®c type-5 phosphodiesterase inhibitor, on human sperm motility, viability, membrane integrity and sperm penetration assay.Spermatozoa were obtained from normal donors (n 6) and infertile men (n 6) were washed using a single Percoll (80%) gradient, suspended in Ham's F-10 medium, and incubated with various doses of sildena®l (125, 250 and 750 ngaml); pentoxifylline (3 mM) was used as a positive control, and Ham's F-10 was used as a reagent control. Sperm motility, grade, viability, membrane integrity (by hypo-osmotic swelling test), and motion evaluation were carried out at various time intervals. Hamster ova sperm penetration assay (SPA) was used to evaluate overall sperm function.Sildena®l did not affect sperm motility, viability or membrane integrity under these conditions as compared to our Ham's control (P b 0.05). Incubation with pentoxifylline signi®cantly enhanced sperm motility (P`0.05) and viability without affecting membrane integrity (P`0.05). Sperm incubated with sildena®l and pentoxifylline from both normal donors and infertile patients demonstrated no signi®cant change in sperm penetration assay from respective controls.In conclusion, sildena®l, at the doses evaluated, did not signi®cantly alter the motility, viability, membrane integrity or sperm penetration characteristics of human spermatozoa from normal donors and infertile patients.

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“…The importance of extracellular Ca 2+ in maintaining motility has been demonstrated in ram sperm [4] and human sperm [13]. An increase in intracellular Ca 2+ , measured by fluorescent Ca 2+ indicators, has been associated with an increase in sperm motility in human [13], hamster [14,15], mouse [1], and ram [5] sperm. A known stimulant of sperm motility, progesterone, increases intracellular Ca 2+ in human sperm [3].…”

Section: R Bains Et Almentioning

confidence: 99%