Relationship Between Metal Transcription Factor-1 and Zinc in Resistance to Metals Producing Free Radicals

Formigari, Alessia; Alberton, Paolo; Cantale, Vera; Nadal, Viviana D.; Feltrin, Moira; Ferronato, Silvia; Santon, Alessandro; Schiavon, Luca; Irato, Paola

doi:10.2174/187231308785739765

Cited by 3 publications

(4 citation statements)

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“…The constitutive ZnT-1 mRNA level significantly increases after Zn treatment. Zn together with other metals such as Cd is able to regulate the ZnT-1 expression by the activation of MTF-1 transcription activity (Langmade et al, 2000;Formigari et al, 2008). It is possible the involvement of a posttranscriptional regulation of ZnT 1 that is not yet demonstrated neither in mammals nor in mollusks.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have reported the Zn ability of interacting with Cu decreasing metal content and retarding oxidative process within cells and tissues (Irato et al, 1996;Irato and Albergoni, 2005;Santon et al, 2006;Formigari et al, 2007). Zn antagonizes in various systems the catalytic properties of the redox-active transition metals Fe and Cu by reducing their ability to generate hydroxyl radical and superoxide (Formigari et al, 2008). Cu accumulation can be counteracted by Zn accumulation; in particular Zn can decrease the Cu content by competitive inhibition of Cu uptake.…”

Section: Introductionmentioning

confidence: 99%

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Metal and metallothionein distribution in different tissues of the Mediterranean clam Venerupis philippinarum during copper treatment and detoxification

Santovito

Boldrin

Irato

2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metal and metallothionein distribution in different tissues of the Mediterranean clam Venerupis philippinarum during copper treatment and detoxification

Santovito

Boldrin

Irato

2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Zn is an essential metal that has been shown to attenuate Cd-induced OS because Zn functions as a cofactor for the antioxidant enzyme copper/zinc superoxide dismutase (Cu/Zn SOD) [255]. Furthermore, Zn increases the expression of MT [256]. MTs are low-molecular-weight proteins with a high affinity for Cd and other heavy metals due to the -SH on their cysteine residues, which aid detoxification [257].…”

Section: Use Of Antioxidants To Mitigate Cd-induced Epigenetic Altera...mentioning

confidence: 99%

Oxidative Stress and Its Role in Cd-Induced Epigenetic Modifications: Use of Antioxidants as a Possible Preventive Strategy

Hernández-Cruz

Arancibia-Hernández

Loyola-Mondragón

et al. 2022

Oxygen

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Oxidative stress (OS) represents one of the main mechanisms of toxicity induced by environmental pollutants such as cadmium (Cd). OS is a natural physiological process where the presence of oxidants, such as reactive oxygen-derived species (ROS), outweighs the strategy of antioxidant defenses, culminating in the interruption of signaling and redox control. It has been suggested that Cd increases ROS mainly by inducing damage to the electron transport chain and by increasing the activity of nicotinamide adenine dinucleotide hydrogen phosphate (NADPH) oxidase (NOX) and the concentration of free iron (Fe), as well as causing a decrease in antioxidant defense. On the other hand, OS has been related to changes in the biology of the epigenome, causing adverse health effects. Recent studies show that Cd generates alterations in deoxyribonucleic acid (DNA) methylation, histone modifications, and noncoding RNA (ncRNA) expression. However, the role of OS in Cd-induced epigenetic modifications is still poorly explored. Therefore, this review provides an update on the basic concepts of OS and its relationship with Cd-induced epigenetic changes. Furthermore, the use of antioxidant compounds is proposed to mitigate Cd-induced epigenetic alterations.

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“…MTF-1 directly coordinates the regulation of genes involved in Zn homeostasis and protection against metal toxicity and regulates the well-coordinated expression of genes in response to metal ions, oxidative stress hypoxia, amino acid starvation and various stress conditions [ 13 ]. These include, but are not limited to, MT [ 14 ], zinc transporter-1 (ZnT-1) [ 15 ] and γ-glutamylcysteine synthetase heavy chain (ɣGCS hc ) [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Cadmium–Zinc Interaction in Mus musculus Fibroblasts

Priante

Pietropoli

Piva

et al. 2022

IJMS

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This work aimed to evaluate the effects of zinc (Zn) relating to cadmium (Cd)-induced toxicity and the role played by MTF-1. This transcription factor regulates the expression of genes encoding metallothioneins (MTs), some Zn transporters and the heavy chain of γ-glutamylcysteine synthetase. For this reason, two cell lines of mouse fibroblasts were used: a wild-type strain and a knockout strain to study the effects. Cells were exposed to complete medium containing: (1) 50 μM ZnSO4 (Zn), (2) 1 μM CdCl2 (Cd 1), (3) 2 μM CdCl2 (Cd 2), (4) 50 μM ZnSO4 + 1 μM CdCl2 (ZnCd 1) and (5) 50 μM ZnSO4 + 2 μM CdCl2 (ZnCd 2) for 4, 18 and 24 h. Following exposure, cell viability, the intracellular content of metals, glutathione (GSH) and MT and the gene expression of the two isoforms of MT was evaluated. The results obtained suggest that a lower Cd content in the co-treatments is responsible for the protection offered by Zn due to the probable competition for a common transporter. Furthermore, Zn determines an increase in GSH in co-treatments compared to treatments with Cd alone. Finally, the MTF-1 factor is essential for the expression of MT-1 but not of MT-2 nor probably for the heavy chain of γ-glutamylcysteine synthetase.

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Relationship Between Metal Transcription Factor-1 and Zinc in Resistance to Metals Producing Free Radicals

Cited by 3 publications

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Metal and metallothionein distribution in different tissues of the Mediterranean clam Venerupis philippinarum during copper treatment and detoxification

Metal and metallothionein distribution in different tissues of the Mediterranean clam Venerupis philippinarum during copper treatment and detoxification

Oxidative Stress and Its Role in Cd-Induced Epigenetic Modifications: Use of Antioxidants as a Possible Preventive Strategy

Cadmium–Zinc Interaction in Mus musculus Fibroblasts

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