Relationship between muscle texture and the crosslinking degree of collagen fibers from octopus ( Octopus vulgaris ), guitarfish ( Rhinobatos productus ), and cazon ( Mustelus lunulatus )

Abstract

Chemical–structural properties and crosslinking degree (lysyl oxidase [Lox] enzymatic activity and pyridinoline [Pyr] content) of collagen isolated from the muscle of octopus (Octopus vulgaris), guitarfish (Rhinobatos productus), and cazon (Mustelus lunulatus) are reported and related with the muscle texture. Histological analysis revealed more collagen fibers stained in the tissues from octopus, which also showed greater shear force (SF) (191.9 ± 38.2 N) compared with the guitarfish (19.7 ± 5.3 N) and cazon (26.2 ± 14.3 N) muscle. The extent of cross‐linked collagen decreased in the order: octopus > guitarfish > cazon. An increase in the total enzymatic activity of the Lox, Pyr content, and insoluble collagen (IC) extraction yield matched with an increase in the SF (p < .01). The Lox activity and Pyr content affect the firmness of the octopus, guitarfish, and cazon muscles, showing the higher crosslinking degree in collagen fibers on octopus. Practical applications The collagen crosslinking degree has been related with the muscle texture of marine organisms. Therefore, the determination of Lox activity and Pyr content may be useful, in a near future, as an indicator of freshness and postcapture development.

Proteomic identification and physicochemical characterisation of paramyosin and collagen from octopus (Octopus vulgaris) and jumbo squid (Dosidicus gigas)

“…The muscle of octopus contains collagen fibres highly crosslinked (Tapia‐Vasquez et al . , 2019); this can be observed in the histological images since the fibres showed more contact in between them. On the other hand, jumbo squid collagen fibres showed an arrangement consistent with previous reports (Hurtado et al .…”

“…, 2018; Tapia‐Vasquez et al . , 2019). These observations can be attributed to the role that the muscle plays in the arms, which is to provide the necessary support and strength for some biological functions, such as hunting.…”

“…Masson’s trichrome stain was carried out following the methodology described by Tapia‐Vasquez et al . (2019). Tissues from octopus and jumbo squid muscle were placed in Bouin’s fixative solution, dehydrated through a graded series (70%–100%) of alcohol using a tissue processor, embedded in paraffin, sectioned into 8.0‐μm‐thick slices using a microtome and then stained using Masson’s trichrome stain.…”

“…The jumbo squid is a decapod that can grow up to 1.9 m in length and weigh up to 50 kg (Torres‐Arreola et al , 2008), while the octopus is an octopod distributed on temperate and tropical waters around the world (Tapia‐Vasquez et al . , 2019). The increasing popularity of Japanese cuisine worldwide has helped increase the demand for squid and octopus; cephalopods represent 11.0% of world commerce in fishery products (FAO, 2018).…”

“…, 2008; Tapia‐Vasquez et al . , 2019). Moreover, currently more than twenty different types of collagen have been identified.…”