2016
DOI: 10.1038/srep37909
|View full text |Cite|
|
Sign up to set email alerts
|

Relationship between nanotopographical alignment and stem cell fate with live imaging and shape analysis

Abstract: The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding of how topographical properties affect cell behavior will allow the rational design of material surfaces that elicit specified biological functions once placed in the body. To this end, we fabricate substrates with aligned or randomly organized fibrous nanostructured topographies. Culturing adipose-derived stem cells (ASCs), we explore the dynamic relationship between the alignment of topograp… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
44
0

Year Published

2017
2017
2021
2021

Publication Types

Select...
9

Relationship

1
8

Authors

Journals

citations
Cited by 54 publications
(45 citation statements)
references
References 52 publications
1
44
0
Order By: Relevance
“…On the other hand, the topography of a biomaterial regulates cellular interactions and determines stem cell fate . Other studies also showed cell growth and differentiation in materials with aligned fibers when compared with random fibers .…”
Section: Resultsmentioning
confidence: 99%
“…On the other hand, the topography of a biomaterial regulates cellular interactions and determines stem cell fate . Other studies also showed cell growth and differentiation in materials with aligned fibers when compared with random fibers .…”
Section: Resultsmentioning
confidence: 99%
“…[6,7] Such approach consists in seeding cells on synthetically fabricated substrates that present ridges or valleys of different size and shape that are able to influence the cell. [10][11][12][13] Therefore micro and nanofabrication of substrates is a powerful tool to explore the mechanisms of cell migration and to study the relation between mechano-sensation and that of the corresponding cell migratory activity from biochemical to macroscopic level. The presence of such patterns at both micro and nanoscale has been shown to influence cytoskeletal reorganization, focal adhesion dynamics, and cell contractility (see, e.g., refs.…”
Section: Doi: 101002/advs201801826mentioning
confidence: 99%
“…The different degrees of cellular modulation by nanochannelar and flat substrates underline that surface topography has an important effect on osteogenic differentiation. Indeed, a large number of studies using different cell types have shown that cellular features such as adhesion, morphology, as well as cytoskeleton organization and osteogenic differentiation, are highly affected by surface topography [25][26][27][28][29][30][31][32]. Thus, nano-or micro-rough Ti surfaces were found to reduce osteoblast cells' proliferation and to enhance differentiation and local factor production, supporting a mature secretory osteoblast-like phenotype [33][34][35][36].…”
Section: Pre-osteoblast Cell Differentiationmentioning
confidence: 99%