Relationship Between Pathogenicity and Phylogeny Based on Restriction Fragment Length Polymorphism inLeptosphaeria maculans

Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia

“…Based on the RFLP profile, these reported species can also be identified. Restriction enzyme EcoRI cuts DNA at 430 bp in agressive isolates (Leptosphaeria maculans), while in non-agressive isolates (Leptosphaeria biglobosa) this fragment was not observed (Koch et al 1991). The obtained polymorphism enabled identification of tested isolates from Serbia by the use of restriction enzymes.…”

“…It was determined, based on RFLP analysis and isoenzymatic analysis, that aggressive isolates form a compact group different from the slightly virulent isolates. The same methods showed that slightly virulent isolates can be divided into three subgroups: NA1, NA2, and NA3 (Koch et al 1991, Gall et al 1995. Gall et al (1995) state that NA1 subgroup is predominant in Europe, whereas NA2 is more common in Canada.…”

Genetics of Brassica rapa (syn. campestris). 2. Multiple disease resistance to three fungal pathogens: Peronospora parasitica, Albugo Candida and Leptosphaeria maculans

“…(Loculoascomycetes, Pleosporales), a facultatively saprophytic, necrotrophic pathogen attacking many crucifers, especially in the genus Brassica (Gabrielson, 1983). Isolate PHW-100, of pathogenicity group 4 (Koch et a!., 1991), was grown on V-8 agar plates under cool-white fluorescent lights at 23°C. All plant and fungal stocks were obtained from the Crucifer Genetics Cooperative, Madison,…”

“…In a world-wide collection of B. rapa genotypes, Mitchell-Olds and Christian (unpublished data) found continuously distributed variation in blackleg resistance, with a positive correlation of 0.55 among levels of blackleg disease caused by L. maculans isolates from pathogenicity groups 3 and 4 (Koch et a!., 1991). No plant genotypes were completely susceptible or completely resistant to either pathotype.…”

The first occurrence of stem canker on oilseed rape caused by Leptosphaeria biglobosa in Serbia

“…The strongest leaf infections were observed at temperatures 15-20 °C and leaf wetness duration for 48 hours (Toscano-Underwood et al, 2001). Based on RFLP analysis non-aggressive isolates can be divided into three subgroups: NA1, NA2, and NA3 (Koch et al, 1991;Gall et al, 1995). Gall et al (1995) state that NA1 subgroup is dominant in Europe, while NA2 is present in Canada.…”