Relationship between the Microbiome and Indoor Temperature/Humidity in a Traditional Japanese House with a Thatched Roof in Kyoto, Japan

Kokubo, Makoto; Fujiyoshi, So; Ogura, Daisuke; Nakajima, Makiko; Fujieda, Ayako; Noda, Jun; Maruyama, Fumito

doi:10.3390/d13100475

Cited by 10 publications

(3 citation statements)

References 51 publications

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“…This positive association highlights the potential impact of housing characteristics on land allocation. In contrast, the presence of thatch roofs showcases a negative relationship, echoing the sentiments of [97,98], who noted that traditional housing materials might correlate with smaller land portions.…”

Section: Discussionmentioning

confidence: 91%

Awareness and Use of Sustainable Land Management Practices in Smallholder Farming Systems

Bwalya,

Mutandwa,

Chiluba

2023

Sustainability

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Sustainable land management (SLM) practices are often touted as a vehicle for simultaneously increasing agricultural productivity and food security in rural areas. In Eastern Zambia, numerous initiatives such as the Zambia Integrated Forest Landscape Project (ZIFLP) have been implemented. Yet, empirical data suggest relatively low levels of SLM uptake in the smallholder farming sector. Therefore, the broad objective of this study was to estimate the relationship between smallholder farmer awareness of SLM technologies and land allocated to SLM at the farm level. We hypothesized the following: H1: Increased farmer awareness of SLM practices leads to more land allocated to SLM activities in Zambia’s Eastern Province; and H2: Adoption of specific SLM practices influences the extent of land allocated to SLM. Using an intra-household cross-sectional survey, data were collected from 761 randomly selected households from 11 chiefdoms of the Eastern Province. The Heckman selection procedure was used to analyze the study’s overarching hypothesis. Findings showed that farmers were generally conversant with SLM as a construct (>90%), with choices being influenced by gender. Conservation agriculture in the form of crop rotations, use of manure, mixed cropping, tree planting, and minimum tillage methods were the most commonly known SLM technologies among farmers. Findings also indicated that awareness is an important antecedent in the use of SLM practices (χ2 = 76.6, p = 0.00), with greater access to extension being positively associated with farmer awareness (p < 0.05). The land allotted to SLM hinged on crop diversity, ownership of different types of livestock, and access to agricultural extension. These findings suggest that long-term commitments to training farmers in SLM is critical. This will be achieved when there is coherence in the information on SLM being given to farmers by all the actors working in the region.

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Section: Discussionmentioning

confidence: 91%

Awareness and Use of Sustainable Land Management Practices in Smallholder Farming Systems

Bwalya,

Mutandwa,

Chiluba

2023

Sustainability

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“… Sampling method Sampler Air flowrate (L/min) Characteristics References Filtration-base High-volume air sampler (HV-500R) Sibata Scientific Technology, Tokyo, Japan 100–800 Size selectivity (PM2.5, PM 10); compatible with quartz fiber and polytetrafluoroethylene filters; collect particles both indoors and outdoors Kawai et al 37 SASS 3100 Dry Air Sampler Research International, Monroe, WA, USA 50–300 Operate with electret filter air samplers; support for sampler-specific semi-automated filter extraction (filter-to-liquid) to generate liquid samples for downstream analysis Bøifot et al 38 AirPort MD8 Air Sampler Sartorius, Gottingen, Germany 50 The gelatin membrane filters (3 μm pore size) can be used in combination with the Sartorius Airport MD8 sampler, which has high retention rate for microorganisms and viruses, and is easy to use and clean in the field Lewandowski et al 39 ; Hasanthi et al 40 Liquid-base BioSampler SKC Inc., Eighty Four, PA, USA 12.5 The classical impinger systems for liquid sample collection. It has been taken as a standard reference sampler for bioaerosol research because of lower microorganism stress Tseng et al 41 ; Dybwad et al 42 Coriolis µ Bertin Technologies, Montigny-le-Bretonneux, France 300 Take just 10 min to collect airborne particles using cyclonic technology paired with a high suction rate; compatible with rapid microbiological analysis methods Kokubo et al 43 ; Carvalho et al 44 SASS 2300 Wetted-wall Air Sampler Research International, Monroe, WA, USA 325 Extracts and transfers airborne pathogens, particulates, bacteria, and spores from sampled air to small water volume for analysis Guo et al 45 BioSpot-VIVA...…”

Section: Sampling Methods Of Low-biomass Bioaerosolsmentioning

confidence: 99%

Perspectives on Sampling and New Generation Sequencing Methods for Low-Biomass Bioaerosols in Atmospheric Environments

et al. 2023

Self Cite

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Bioaerosols play essential roles in the atmospheric environment and can affect human health. With a few exceptions (e.g., farm or rainforest environments), bioaerosol samples from wide-ranging environments typically have a low biomass, including bioaerosols from indoor environments (e.g., residential homes, offices, or hospitals), outdoor environments (e.g., urban or rural air). Some specialized environments (e.g., clean rooms, the Earth’s upper atmosphere, or the international space station) have an ultra-low-biomass. This review discusses the primary sources of bioaerosols and influencing factors, the recent advances in air sampling techniques and the new generation sequencing (NGS) methods used for the characterization of low-biomass bioaerosol communities, and challenges in terms of the bias introduced by different air samplers when samples are subjected to NGS analysis with a focus on ultra-low biomass. High-volume filter-based or liquid-based air samplers compatible with NGS analysis are required to improve the bioaerosol detection limits for microorganisms. A thorough understanding of the performance and outcomes of bioaerosol sampling using NGS methods and a robust protocol for aerosol sample treatment for NGS analysis are needed. Advances in NGS techniques and bioinformatic tools will contribute toward the precise high-throughput identification of the taxonomic profiles of bioaerosol communities and the determination of their functional and ecological attributes in the atmospheric environment. In particular, long-read amplicon sequencing, viability PCR, and meta-transcriptomics are promising techniques for discriminating and detecting pathogenic microorganisms that may be active and infectious in bioaerosols and, therefore, pose a threat to human health. Supplementary Information The online version contains supplementary material available at 10.1007/s41745-023-00380-x.

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“…Despite these advantages, the application of transcriptomics in indoor surface microbiome studies has been sparse 12 . Current indoor surface microbiome studies only focus on taxonomic profiling and functional potentials that recovered from (meta)genomics analysis where the importance of gene expression levels is overlooked or yet to be addressed 13, 14 . Transcriptomics analysis from this study can provide insights on the response of pathogens to tested cleaning scenarios on indoor surfaces.…”

Section: Introductionmentioning

confidence: 99%

Clinically Relevant Pathogens on Surfaces Display Differences in Survival and Transcriptomic Response in Relation to Probiotic and Traditional Cleaning Strategies

Shuai

Sumner

et al. 2022

Preprint

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Prolonged survival of clinically relevant pathogens on inanimate surfaces represents a major concern in healthcare facilities. Contaminated surfaces can serve as reservoirs of potential pathogens and greatly hinder the prevention of healthcare-associated infections. Probiotic cleaning using environmental microorganisms to promote inter-species competition has been proposed as an alternative to traditional chemical-based cleaning using antimicrobials. Probiotic cleaning seeks to take advantage of ecological principles such as competitive exclusion and utilize benign microorganisms to inhibit viable pathogens on indoor surfaces. However, limited mechanistic study has yielded direct evidence that enables the scientific community to understand the stress response, or microbe-microbe interactions between healthcare-associated pathogens and probiotic bacteria. Therefore, to bridge this knowledge gap, we combined transcriptomics and traditional microbiology techniques to investigate the differential impact of chemical-based and probiotic surface cleaners on the survival of Acinetobacter baumannii and Klebsiella pneumoniae, two clinically important pathogens. Although probiotic Bacillus included in a commercialized All-Purpose Probiotic Cleaner persisted on surfaces for an extended period of time, surfaces contaminated with A. baumannii cleaned using chemical-based detergent with and without probiotic Bacillus showed no statistical difference in the viable colony forming units (CFUs) of A. baumannii. Similarly, for Klebsiella pneumoniae, there were negligible statistical differences in CFUs between probiotic and detergent cleaning scenarios. The transcriptome of A. baumannii with and without probiotic addition shared a high degree of similarity in overall gene expression, while the transcriptome of K. pneumoniae with and without probiotic addition differed in overall gene expression. Together, these results highlight the need to fully understand the underlying biological and ecological mechanisms for different pathogens and practical implications of probiotic indoor cleaning.

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Relationship between the Microbiome and Indoor Temperature/Humidity in a Traditional Japanese House with a Thatched Roof in Kyoto, Japan

Cited by 10 publications

References 51 publications

Awareness and Use of Sustainable Land Management Practices in Smallholder Farming Systems

Awareness and Use of Sustainable Land Management Practices in Smallholder Farming Systems

Perspectives on Sampling and New Generation Sequencing Methods for Low-Biomass Bioaerosols in Atmospheric Environments

Clinically Relevant Pathogens on Surfaces Display Differences in Survival and Transcriptomic Response in Relation to Probiotic and Traditional Cleaning Strategies

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