Relationship between the Phylogenetic Group of Escherichia coli Strains Isolated in Water and Fish in Fresco Lagoon (Côte d’Ivoire)

Kouadio-Ngbesso, Nirupa Nadège; Kouassi, Nazaire; N’guessan, Kouadio Florent; Adingra, A.A.; Yobouet, Bassa Antoine; Dadié, Adjehi; Djé, Koffi Marcellin

doi:10.20546/ijcmas.2016.510.047

Cited by 2 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…E. coli is a normal habitant of warm-blooded animals. Although not a normal host of fish flora, it has been isolated from stomach, gut, gills, muscle, and skin of fish (Janssen, 1970;Hejkel et al, 1983;Ogbondeminu, 1993;Kouadio-Ngbesso et al, 2016;Ribeiro et al, 2016). The presence of E. coli in viscera and gills in Layo's fish may be due to the quality of water in which they are cultured.…”

Section: Frequency Of E Coli Strains Isolatedmentioning

confidence: 99%

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

̈ge¹,

Mireille²,

RenÃ³

et al. 2019

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The objective of this study was to know the sanitary quality of fish coming from Layo farm. Twenty fishes (Oreochromis niloticus), were selected in four ponds and Escherichia coli were isolated in gills and viscera according to microbiological methods. One hundred and twenty strains of E. coli were isolated, and their virulence was performed by polymerase chain reaction (PCR) using specific primers. Eleven (11) strains (9.16%) including 7 strains of gills (11.66%) and 4 strains of viscera (6.66%) had virulence genes eae, Stx1, Stx2 or ial. Atypical Enteropathogenic E. coli (EPEC, eae+, lack of bfp) was isolated from gills (5%) and viscera (1.66%). Shiga toxigenic E. coli (STEC) with genes eae + Stx2, Stx1 and Stx2 were isolated in viscera (5%) and gills (3.33%). For Enteroinvasive E. coli (EIEC), ial gene was isolated in gills (3.33%) but no ipah gene. Enterotoxinogen E. coli (ETEC) with lt gene and Enteroaggregating E. coli (EAEC) with aggA gene were not detected in this study. This study revealed that some fish from Layo farm are carriers of virulent E. coli that can cause serious human diseases and can lead to consumer death if cooking is insufficient or by cross-contamination. This therefore poses a real public health problem.

show abstract

Section: Frequency Of E Coli Strains Isolatedmentioning

confidence: 99%

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

̈ge¹,

Mireille²,

RenÃ³

et al. 2019

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Pathogenic strains have been associated with several diseases, including diarrhea, urinary tract infections, and meningitis [12]. In a recent study, E. coli belonging to phylogenetics groups A, B1, and B2 were isolated from fishes in Fresco lagoon [13]. The presence of E. coli represents a risk of disease if strains are found to be pathogenic.…”

Section: Introductionmentioning

confidence: 99%

Comparative Biotypic and Phylogenetic Profiles ofEscherichia coliIsolated from Resident Stool and Lagoon in Fresco (Côte d’Ivoire)

Kouadio-Ngbesso

Koua

Attien

et al. 2019

International Journal of Microbiology

View full text Add to dashboard Cite

Anthropogenic activities could expose Fresco lagoon to microbial pollution. The objective of this study was to determine the level of pollution in Fresco lagoon related to fecal contaminations. Two hundred and seventy (270) samples including 216 water and 54 human stools samples from local residents were collected. Escherichia coli was isolated and identified according to classical bacteriology procedure. Strains were characterized by biotyping on API 20E gallery and phylogenetic typing by PCR triplex of Clermont. A set of 392 strains of E. coli was distributed into 18 biotypic profiles. Five biotypes were common to water and human. Classification of all biotypes revealed close relationship between water and human strains because of their repartition in the same groups. Phylogenetic groups A, B1, B2, and D were identified in all strains. Strains belonging to phylogenetic group A were most frequent in water (69.82%) and human stool (44.44%) followed by group B1 in water (24%) and human stool (40.7%). Strains of group B2 were scarce in water (4.4%) and humans (7.41%). The diversity of E. coli biotypes observed in this study revealed animal and human origins of contaminations. A close relationship was found between water and human strains, and the presence of commensal and extraintestinal pathogenic E. coli in all samples could represent a potential reservoir of extraintestinal infections for resident populations.

show abstract

Relationship between the Phylogenetic Group of Escherichia coli Strains Isolated in Water and Fish in Fresco Lagoon (Côte d’Ivoire)

Cited by 2 publications

References 16 publications

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

Comparative Biotypic and Phylogenetic Profiles ofEscherichia coliIsolated from Resident Stool and Lagoon in Fresco (Côte d’Ivoire)

Contact Info

Product

Resources

About