Relationship of Cultivar Resistance to Distribution of<i>Verticillium dahliae</i>in Inoculated Cotton Plants and to Growth of Single Conidia on Excised Stem Segments

Garas, N. A.

doi:10.1094/phyto-76-1005

Cited by 17 publications

(14 citation statements)

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“…After the entry of the root cortex through wounds or by penetrating epidermal cells, the fungus reaches the vascular tissue by crossing the endodermis (Schnathorst, 1981;Vallad and Subbarao, 2008). Systemic invasion occurs when a large number of conidia are produced and transported through the xylem to the aerial parts of the plants (Garas et al, 1986;Vallad and Subbarao, 2008). Resulting disease symptoms have been attributed to water stress caused by the occlusion of xylem vessels and the production of phytotoxins and ethylene (Buchner et al, 1982;Cronshaw and Pegg, 1976;Cooper and Durrands, 1989;DeVay, 1989;Pegg, 1981).…”

Section: Introductionmentioning

confidence: 99%

Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Tzima

Paplomatas

Rauyaree

et al. 2010

Fungal Genetics and Biology

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Section: Introductionmentioning

confidence: 99%

Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Tzima

Paplomatas

Rauyaree

et al. 2010

Fungal Genetics and Biology

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“…When the fungus ingress the cotton plant, the pathogens always first colonize on the root surface [4,5], then directly penetrate into the root or the stem through wounded areas. Early studies showed that the PR protein and phytoalexins were detected much more early in roots of resistant lines than susceptible lines.…”

Section: Discussionmentioning

confidence: 99%

“…When V. dahliae pathogens penetrated directly into cotton epidermal cells, parenchyma cells of the vascular tissues of resistant variety were reinforced by the callose, cellulose and polysaccharides [1][2][3][4][5]. The high concentration of terpenoids and phenolic substances were accumulated in these cells, which then coated pathogens within the vessels to stop fungal ingression [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton

Chen¹,

Sun²,

Wang³

et al. 2013

AJPS

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Early stage expression of PR10 combined with phytoalexins contributed to Verticillium wilt resistance in cotton. In order to analysis the activities of PR10 proteins during pathogens' infection, we cloned a Verticillium-induced PR10 (GbPR10-1) gene from cotton (Gossypium barbadense) and compared its expression patterns and domains with other PR10 proteins. Bioinformatics indicated that GbPR10-1 showed the lowest similarity with other 12 different PR10 genes in cotton (Upland and sea-island cotton). Expression profiles showed that GbPR10-1 gene instantly up-regulated after infection by V. dahliae in the sea-island cotton plants. GbPR10-1 was also induced by environmental stimulus including heat, submergence and salt, and ethylene but not by ABA and salicylic acid. The GbPR10-1 protein expressed in E. coli BL21 demonstrated that it had a low ribonuclease-like activity in vitro, and could inhibit V. dahliae hyphae growth but not its spores. Comparison analysis of GbPR10-1 (from resistant species) and GhPR10-1 (from susceptible species) responding to V. dahliae infection, only GbPR10-1 gene was strongly induced in the sea-island cotton plants (incompatible response), indicating that PR10-1 genes was linked to resistance signal. In summary, the earlier activation of GbPR10-1 gene, as the index of resistance response, would be aid to block the V. dahliae attack in cotton.

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“…Verticillium wilt caused by the soil-borne fungal pathogen V. dahliae Kleb is among the most prevalent and lethal diseases in cotton ( Daayf et al, 1995 ; Fradin and Thomma, 2006 ; Klosterman et al, 2009 ). V. dahliae colonizes the plant through young, uninjured roots or puncture wounds to the xylem and causes browning of the vasculature, leaf discoloration, wilting, and defoliation ( Garas et al, 1986 ). Verticillium wilt is difficult to control due to the viability and persistence of V. dahliae microsclerotia in soil ( Fradin and Thomma, 2006 ) and shortage of resistance cotton germplasms ( Yang et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

GhSNAP33, a t-SNARE Protein From Gossypium hirsutum, Mediates Resistance to Verticillium dahliae Infection and Tolerance to Drought Stress

et al. 2018

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Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins mediate membrane fusion and deliver cargo to specific cellular locations through vesicle trafficking. Synaptosome-associated protein of 25 kDa (SNAP25) is a target membrane SNARE that drives exocytosis by fusing plasma and vesicular membranes. In this study, we isolated GhSNAP33, a gene from cotton (Gossypium hirsutum), encoding a SNAP25-type protein containing glutamine (Q)b- and Qc-SNARE motifs connected by a linker. GhSNAP33 expression was induced by H2O2, salicylic acid, abscisic acid, and polyethylene glycol 6000 treatment and Verticillium dahliae inoculation. Ectopic expression of GhSNAP33 enhanced the tolerance of yeast cells to oxidative and osmotic stresses. Virus-induced gene silencing of GhSNAP33 induced spontaneous cell death and reactive oxygen species accumulation in true leaves at a later stage of cotton development. GhSNAP33-deficient cotton was susceptible to V. dahliae infection, which resulted in severe wilt on leaves, an elevated disease index, enhanced vascular browning and thylose accumulation. Conversely, Arabidopsis plants overexpressing GhSNAP33 showed significant resistance to V. dahliae, with reduced disease index and fungal biomass and elevated expression of PR1 and PR5. Leaves from GhSNAP33-transgenic plants showed increased callose deposition and reduced mycelia growth. Moreover, GhSNAP33 overexpression enhanced drought tolerance in Arabidopsis, accompanied with reduced water loss rate and enhanced expression of DERB2A and RD29A during dehydration. Thus, GhSNAP33 positively mediates plant defense against stress conditions and V. dahliae infection, rendering it a candidate for the generation of stress-resistant engineered cotton.

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Relationship of Cultivar Resistance to Distribution ofVerticillium dahliaein Inoculated Cotton Plants and to Growth of Single Conidia on Excised Stem Segments

Cited by 17 publications

References 0 publications

Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton

GhSNAP33, a t-SNARE Protein From Gossypium hirsutum, Mediates Resistance to Verticillium dahliae Infection and Tolerance to Drought Stress

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Relationship of Cultivar Resistance to Distribution ofVerticillium dahliaein Inoculated Cotton Plants and to Growth of Single Conidia on Excised Stem Segments

Cited by 17 publications

References 0 publications

Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae

Functional Analysis of a Wilt Fungus Inducible &lt;i&gt;PR&lt;/i&gt;10-1 Gene from Cotton

GhSNAP33, a t-SNARE Protein From Gossypium hirsutum, Mediates Resistance to Verticillium dahliae Infection and Tolerance to Drought Stress

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Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton